Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20545 and BWI76_RS20550 are separated by 202 nucleotides BWI76_RS20550 and BWI76_RS20555 are separated by 41 nucleotides BWI76_RS20555 and BWI76_RS20560 are separated by 219 nucleotides BWI76_RS20560 and BWI76_RS20565 are separated by 4 nucleotides BWI76_RS20565 and BWI76_RS20570 are separated by 57 nucleotides
BWI76_RS20545: BWI76_RS20545 - sensor domain-containing phosphodiesterase, at 4,303,715 to 4,305,895
_RS20545
BWI76_RS20550: BWI76_RS20550 - tRNA-Ala, at 4,306,098 to 4,306,173
_RS20550
BWI76_RS20555: BWI76_RS20555 - tRNA-Ala, at 4,306,215 to 4,306,290
_RS20555
BWI76_RS20560: BWI76_RS20560 - hypothetical protein, at 4,306,510 to 4,306,863
_RS20560
BWI76_RS20565: BWI76_RS20565 - hypothetical protein, at 4,306,868 to 4,307,263
_RS20565
BWI76_RS20570: BWI76_RS20570 - glutamate--tRNA ligase, at 4,307,321 to 4,308,739
_RS20570
Position (kb)
4306
4307
4308 Strain fitness (log2 ratio)
-1
0
1 at 4305.878 kb on - strand at 4305.956 kb on + strand at 4306.006 kb on + strand at 4306.019 kb on - strand at 4306.021 kb on - strand at 4306.344 kb on + strand at 4306.509 kb on - strand at 4306.531 kb on - strand at 4306.563 kb on - strand, within BWI76_RS20560 at 4306.637 kb on - strand, within BWI76_RS20560 at 4306.639 kb on - strand, within BWI76_RS20560 at 4307.110 kb on - strand, within BWI76_RS20565 at 4307.155 kb on - strand, within BWI76_RS20565 at 4307.185 kb on - strand, within BWI76_RS20565 at 4307.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 4,305,878 - +0.7 4,305,956 + -0.9 4,306,006 + +0.3 4,306,019 - +0.4 4,306,021 - -0.9 4,306,344 + +0.2 4,306,509 - +0.1 4,306,531 - +0.2 4,306,563 - BWI76_RS20560 0.15 +0.2 4,306,637 - BWI76_RS20560 0.36 +0.2 4,306,639 - BWI76_RS20560 0.36 +0.2 4,307,110 - BWI76_RS20565 0.61 -1.1 4,307,155 - BWI76_RS20565 0.72 -1.0 4,307,185 - BWI76_RS20565 0.80 +0.5 4,307,247 - +0.0
Or see this region's nucleotide sequence