Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03625

Experiment: Ammonium chloride (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03615 and BWI76_RS03620 are separated by 17 nucleotidesBWI76_RS03620 and BWI76_RS03625 are separated by 147 nucleotidesBWI76_RS03625 and BWI76_RS03630 are separated by 87 nucleotidesBWI76_RS03630 and BWI76_RS03635 overlap by 7 nucleotides BWI76_RS03615: BWI76_RS03615 - putative transcriptional regulatory protein, at 747,759 to 748,559 _RS03615 BWI76_RS03620: BWI76_RS03620 - TetR family transcriptional regulator, at 748,577 to 749,161 _RS03620 BWI76_RS03625: BWI76_RS03625 - NAD(P)H quinone oxidoreductase, at 749,309 to 750,091 _RS03625 BWI76_RS03630: BWI76_RS03630 - LysR family transcriptional regulator, at 750,179 to 751,087 _RS03630 BWI76_RS03635: BWI76_RS03635 - FosA family fosfomycin resistance glutathione transferase, at 751,081 to 751,524 _RS03635 Position (kb) 749 750 751Strain fitness (log2 ratio) -1 0 1 2 3at 748.369 kb on + strand, within BWI76_RS03615at 748.574 kb on + strandat 748.728 kb on + strand, within BWI76_RS03620at 748.814 kb on - strand, within BWI76_RS03620at 748.823 kb on + strand, within BWI76_RS03620at 748.823 kb on + strand, within BWI76_RS03620at 748.859 kb on + strand, within BWI76_RS03620at 748.867 kb on - strand, within BWI76_RS03620at 749.145 kb on - strandat 749.219 kb on - strandat 749.320 kb on - strandat 749.376 kb on - strandat 749.381 kb on + strandat 749.483 kb on - strand, within BWI76_RS03625at 749.530 kb on + strand, within BWI76_RS03625at 749.538 kb on - strand, within BWI76_RS03625at 749.568 kb on + strand, within BWI76_RS03625at 749.610 kb on + strandat 749.739 kb on + strand, within BWI76_RS03625at 749.739 kb on + strand, within BWI76_RS03625at 749.754 kb on + strand, within BWI76_RS03625at 749.757 kb on - strand, within BWI76_RS03625at 749.970 kb on + strand, within BWI76_RS03625at 749.978 kb on - strand, within BWI76_RS03625at 749.984 kb on + strand, within BWI76_RS03625at 750.123 kb on + strandat 750.263 kb on - strandat 750.294 kb on + strand, within BWI76_RS03630at 750.475 kb on - strand, within BWI76_RS03630at 750.534 kb on - strand, within BWI76_RS03630at 750.548 kb on + strand, within BWI76_RS03630at 750.734 kb on + strand, within BWI76_RS03630at 750.734 kb on + strand, within BWI76_RS03630at 750.751 kb on + strand, within BWI76_RS03630at 750.880 kb on + strand, within BWI76_RS03630at 750.948 kb on + strand, within BWI76_RS03630at 751.005 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N)
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748,369 + BWI76_RS03615 0.76 +0.1
748,574 + -0.1
748,728 + BWI76_RS03620 0.26 +0.7
748,814 - BWI76_RS03620 0.41 -0.4
748,823 + BWI76_RS03620 0.42 -0.4
748,823 + BWI76_RS03620 0.42 +0.1
748,859 + BWI76_RS03620 0.48 -0.6
748,867 - BWI76_RS03620 0.50 +0.2
749,145 - +0.5
749,219 - -0.9
749,320 - -0.2
749,376 - -0.8
749,381 + +2.9
749,483 - BWI76_RS03625 0.22 +1.4
749,530 + BWI76_RS03625 0.28 +0.2
749,538 - BWI76_RS03625 0.29 +0.1
749,568 + BWI76_RS03625 0.33 -0.2
749,610 + -0.9
749,739 + BWI76_RS03625 0.55 +0.5
749,739 + BWI76_RS03625 0.55 -0.9
749,754 + BWI76_RS03625 0.57 +0.9
749,757 - BWI76_RS03625 0.57 +1.6
749,970 + BWI76_RS03625 0.84 +1.2
749,978 - BWI76_RS03625 0.85 +0.4
749,984 + BWI76_RS03625 0.86 +1.4
750,123 + +0.3
750,263 - -0.1
750,294 + BWI76_RS03630 0.13 -0.0
750,475 - BWI76_RS03630 0.33 +0.0
750,534 - BWI76_RS03630 0.39 -0.2
750,548 + BWI76_RS03630 0.41 +0.2
750,734 + BWI76_RS03630 0.61 +0.7
750,734 + BWI76_RS03630 0.61 -1.2
750,751 + BWI76_RS03630 0.63 +0.7
750,880 + BWI76_RS03630 0.77 +2.3
750,948 + BWI76_RS03630 0.85 +0.3
751,005 + -0.4

Or see this region's nucleotide sequence