Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19515 and BWI76_RS19520 are separated by 97 nucleotides BWI76_RS19520 and BWI76_RS19525 are separated by 138 nucleotides BWI76_RS19525 and BWI76_RS19530 are separated by 174 nucleotides BWI76_RS19530 and BWI76_RS19535 are separated by 155 nucleotides
BWI76_RS19515: BWI76_RS19515 - D-lactate dehydrogenase (cytochrome c-553) (EC 1.1.2.5) (from data) , at 4,078,765 to 4,080,507
_RS19515
BWI76_RS19520: BWI76_RS19520 - acetyltransferase GCN5, at 4,080,605 to 4,081,159
_RS19520
BWI76_RS19525: BWI76_RS19525 - D-alanyl-D-alanine endopeptidase, at 4,081,298 to 4,082,245
_RS19525
BWI76_RS19530: BWI76_RS19530 - YIP1 family protein, at 4,082,420 to 4,083,007
_RS19530
BWI76_RS19535: BWI76_RS19535 - putative integral membrane protein, at 4,083,163 to 4,083,732
_RS19535
Position (kb)
4081
4082
4083 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4080.333 kb on + strand at 4080.371 kb on - strand at 4080.419 kb on + strand at 4080.523 kb on + strand at 4080.542 kb on + strand at 4080.550 kb on - strand at 4080.699 kb on - strand, within BWI76_RS19520 at 4080.728 kb on - strand, within BWI76_RS19520 at 4080.884 kb on - strand, within BWI76_RS19520 at 4081.612 kb on + strand, within BWI76_RS19525 at 4082.036 kb on - strand, within BWI76_RS19525 at 4082.209 kb on + strand at 4082.217 kb on - strand at 4082.217 kb on - strand at 4082.307 kb on - strand at 4082.309 kb on - strand at 4082.309 kb on - strand at 4082.426 kb on + strand at 4082.455 kb on - strand at 4082.510 kb on + strand, within BWI76_RS19530 at 4082.580 kb on + strand, within BWI76_RS19530 at 4082.580 kb on + strand, within BWI76_RS19530 at 4082.801 kb on - strand, within BWI76_RS19530 at 4082.925 kb on - strand, within BWI76_RS19530 at 4083.031 kb on + strand at 4083.039 kb on - strand at 4083.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 4,080,333 + -0.8 4,080,371 - +1.1 4,080,419 + +0.6 4,080,523 + +0.1 4,080,542 + -2.1 4,080,550 - -0.8 4,080,699 - BWI76_RS19520 0.17 +0.0 4,080,728 - BWI76_RS19520 0.22 +1.0 4,080,884 - BWI76_RS19520 0.50 -0.1 4,081,612 + BWI76_RS19525 0.33 +0.3 4,082,036 - BWI76_RS19525 0.78 -1.8 4,082,209 + -2.3 4,082,217 - +1.2 4,082,217 - -3.4 4,082,307 - -0.1 4,082,309 - -0.7 4,082,309 - +0.2 4,082,426 + -0.3 4,082,455 - +0.3 4,082,510 + BWI76_RS19530 0.15 -0.6 4,082,580 + BWI76_RS19530 0.27 +0.6 4,082,580 + BWI76_RS19530 0.27 -0.2 4,082,801 - BWI76_RS19530 0.65 +0.6 4,082,925 - BWI76_RS19530 0.86 -1.9 4,083,031 + +0.7 4,083,039 - +0.8 4,083,120 + -0.0
Or see this region's nucleotide sequence