Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10730

Experiment: Glucuronamide (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10720 and BWI76_RS10725 are separated by 516 nucleotidesBWI76_RS10725 and BWI76_RS10730 are separated by 139 nucleotidesBWI76_RS10730 and BWI76_RS10735 are separated by 147 nucleotidesBWI76_RS10735 and BWI76_RS10740 are separated by 19 nucleotides BWI76_RS10720: BWI76_RS10720 - putrescine importer, at 2,265,191 to 2,266,582 _RS10720 BWI76_RS10725: BWI76_RS10725 - hypothetical protein, at 2,267,099 to 2,267,323 _RS10725 BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710 _RS10730 BWI76_RS10735: BWI76_RS10735 - hypothetical protein, at 2,268,858 to 2,269,085 _RS10735 BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701 _RS10740 Position (kb) 2267 2268 2269Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2266.492 kb on - strandat 2266.506 kb on - strandat 2266.551 kb on - strandat 2266.567 kb on + strandat 2266.567 kb on + strandat 2266.570 kb on - strandat 2266.598 kb on + strandat 2266.739 kb on - strandat 2266.776 kb on + strandat 2266.776 kb on + strandat 2267.058 kb on + strandat 2267.064 kb on + strandat 2267.064 kb on + strandat 2267.066 kb on - strandat 2267.148 kb on - strand, within BWI76_RS10725at 2267.264 kb on - strand, within BWI76_RS10725at 2267.502 kb on + strandat 2267.510 kb on - strandat 2267.535 kb on - strandat 2267.592 kb on - strandat 2267.728 kb on - strand, within BWI76_RS10730at 2267.861 kb on - strand, within BWI76_RS10730at 2268.911 kb on - strand, within BWI76_RS10735at 2269.145 kb on + strandat 2269.145 kb on + strandat 2269.610 kb on - strand, within BWI76_RS10740at 2269.634 kb on - strand, within BWI76_RS10740at 2269.652 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Glucuronamide (N)
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2,266,492 - -0.7
2,266,506 - -0.7
2,266,551 - +0.7
2,266,567 + +0.1
2,266,567 + -0.1
2,266,570 - -0.7
2,266,598 + +0.3
2,266,739 - -1.1
2,266,776 + -3.3
2,266,776 + +4.4
2,267,058 + -0.2
2,267,064 + -0.3
2,267,064 + +0.4
2,267,066 - -2.1
2,267,148 - BWI76_RS10725 0.22 +0.4
2,267,264 - BWI76_RS10725 0.73 -0.7
2,267,502 + +0.7
2,267,510 - +0.2
2,267,535 - -1.5
2,267,592 - -0.5
2,267,728 - BWI76_RS10730 0.21 +2.8
2,267,861 - BWI76_RS10730 0.32 +0.5
2,268,911 - BWI76_RS10735 0.23 +0.3
2,269,145 + +0.1
2,269,145 + -1.2
2,269,610 - BWI76_RS10740 0.85 -0.2
2,269,634 - BWI76_RS10740 0.89 -0.9
2,269,652 + +0.4

Or see this region's nucleotide sequence