Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5262 and Pf6N2E2_5263 are separated by 121 nucleotides Pf6N2E2_5263 and Pf6N2E2_5264 are separated by 215 nucleotides Pf6N2E2_5264 and Pf6N2E2_5265 overlap by 33 nucleotides Pf6N2E2_5265 and Pf6N2E2_5266 are separated by 137 nucleotides
Pf6N2E2_5262 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 6,057,501 to 6,057,986
_5262
Pf6N2E2_5263 - Glutathione peroxidase family protein, at 6,058,108 to 6,058,590
_5263
Pf6N2E2_5264 - hypothetical protein, at 6,058,806 to 6,058,940
_5264
Pf6N2E2_5265 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 6,058,908 to 6,060,011
_5265
Pf6N2E2_5266 - Glycosyltransferase, at 6,060,149 to 6,061,348
_5266
Position (kb)
6058
6059
6060
6061 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6057.997 kb on - strand at 6058.044 kb on + strand at 6058.058 kb on + strand at 6058.058 kb on + strand at 6058.059 kb on - strand at 6058.059 kb on - strand at 6058.128 kb on + strand at 6058.128 kb on + strand at 6058.129 kb on - strand at 6058.232 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.470 kb on + strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.672 kb on + strand at 6058.690 kb on + strand at 6058.807 kb on + strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.815 kb on - strand at 6058.830 kb on + strand, within Pf6N2E2_5264 at 6058.837 kb on - strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.863 kb on - strand, within Pf6N2E2_5264 at 6058.915 kb on + strand at 6058.916 kb on - strand at 6058.916 kb on - strand at 6059.034 kb on + strand, within Pf6N2E2_5265 at 6059.034 kb on + strand, within Pf6N2E2_5265 at 6059.035 kb on - strand, within Pf6N2E2_5265 at 6059.035 kb on - strand, within Pf6N2E2_5265 at 6059.038 kb on - strand, within Pf6N2E2_5265 at 6059.136 kb on + strand, within Pf6N2E2_5265 at 6059.136 kb on + strand, within Pf6N2E2_5265 at 6059.137 kb on - strand, within Pf6N2E2_5265 at 6059.183 kb on - strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.598 kb on + strand, within Pf6N2E2_5265 at 6059.673 kb on - strand, within Pf6N2E2_5265 at 6060.009 kb on + strand at 6060.010 kb on - strand at 6060.031 kb on + strand at 6060.032 kb on - strand at 6060.032 kb on - strand at 6060.058 kb on + strand at 6060.058 kb on + strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.059 kb on - strand at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.366 kb on + strand, within Pf6N2E2_5266 at 6060.367 kb on - strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.982 kb on + strand, within Pf6N2E2_5266
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 6,057,997 - +0.3 6,058,044 + -0.3 6,058,058 + -1.7 6,058,058 + -0.6 6,058,059 - +0.6 6,058,059 - -0.7 6,058,128 + -0.5 6,058,128 + -0.7 6,058,129 - -0.5 6,058,232 - Pf6N2E2_5263 0.26 -1.3 6,058,253 - Pf6N2E2_5263 0.30 -0.6 6,058,253 - Pf6N2E2_5263 0.30 +0.1 6,058,253 - Pf6N2E2_5263 0.30 +0.4 6,058,261 + Pf6N2E2_5263 0.32 -0.6 6,058,261 + Pf6N2E2_5263 0.32 +0.4 6,058,261 + Pf6N2E2_5263 0.32 -1.7 6,058,262 - Pf6N2E2_5263 0.32 -0.5 6,058,262 - Pf6N2E2_5263 0.32 +0.2 6,058,262 - Pf6N2E2_5263 0.32 -0.3 6,058,262 - Pf6N2E2_5263 0.32 +1.3 6,058,262 - Pf6N2E2_5263 0.32 +0.2 6,058,262 - Pf6N2E2_5263 0.32 -0.4 6,058,262 - Pf6N2E2_5263 0.32 -2.9 6,058,262 - Pf6N2E2_5263 0.32 -0.9 6,058,470 + Pf6N2E2_5263 0.75 -1.3 6,058,471 - Pf6N2E2_5263 0.75 +2.1 6,058,471 - Pf6N2E2_5263 0.75 -1.3 6,058,672 + +0.6 6,058,690 + -1.2 6,058,807 + +0.5 6,058,808 - +0.4 6,058,808 - +0.9 6,058,808 - -0.2 6,058,815 - +0.3 6,058,830 + Pf6N2E2_5264 0.18 -0.9 6,058,837 - Pf6N2E2_5264 0.23 -0.5 6,058,859 + Pf6N2E2_5264 0.39 -0.6 6,058,859 + Pf6N2E2_5264 0.39 -0.8 6,058,859 + Pf6N2E2_5264 0.39 -0.0 6,058,859 + Pf6N2E2_5264 0.39 +0.3 6,058,860 - Pf6N2E2_5264 0.40 -0.2 6,058,860 - Pf6N2E2_5264 0.40 -1.3 6,058,860 - Pf6N2E2_5264 0.40 -0.6 6,058,863 - Pf6N2E2_5264 0.42 -0.6 6,058,915 + -0.7 6,058,916 - +0.3 6,058,916 - +1.1 6,059,034 + Pf6N2E2_5265 0.11 +0.1 6,059,034 + Pf6N2E2_5265 0.11 -0.8 6,059,035 - Pf6N2E2_5265 0.12 -0.7 6,059,035 - Pf6N2E2_5265 0.12 -0.3 6,059,038 - Pf6N2E2_5265 0.12 -0.3 6,059,136 + Pf6N2E2_5265 0.21 +0.0 6,059,136 + Pf6N2E2_5265 0.21 -0.8 6,059,137 - Pf6N2E2_5265 0.21 -1.1 6,059,183 - Pf6N2E2_5265 0.25 -0.7 6,059,598 + Pf6N2E2_5265 0.62 -0.3 6,059,598 + Pf6N2E2_5265 0.62 -0.6 6,059,673 - Pf6N2E2_5265 0.69 +0.4 6,060,009 + -0.3 6,060,010 - +1.4 6,060,031 + +0.6 6,060,032 - -0.4 6,060,032 - +0.0 6,060,058 + -0.7 6,060,058 + -1.5 6,060,059 - -0.5 6,060,059 - -0.6 6,060,059 - -0.1 6,060,059 - -0.4 6,060,059 - -1.0 6,060,366 + Pf6N2E2_5266 0.18 +0.7 6,060,366 + Pf6N2E2_5266 0.18 +0.2 6,060,366 + Pf6N2E2_5266 0.18 +2.1 6,060,367 - Pf6N2E2_5266 0.18 -0.2 6,060,405 + Pf6N2E2_5266 0.21 +0.1 6,060,405 + Pf6N2E2_5266 0.21 +0.3 6,060,406 - Pf6N2E2_5266 0.21 -1.6 6,060,406 - Pf6N2E2_5266 0.21 +0.1 6,060,501 + Pf6N2E2_5266 0.29 +0.5 6,060,501 + Pf6N2E2_5266 0.29 +0.1 6,060,894 + Pf6N2E2_5266 0.62 -1.4 6,060,894 + Pf6N2E2_5266 0.62 +0.6 6,060,895 - Pf6N2E2_5266 0.62 +0.5 6,060,895 - Pf6N2E2_5266 0.62 -0.8 6,060,895 - Pf6N2E2_5266 0.62 +0.2 6,060,906 - Pf6N2E2_5266 0.63 -1.0 6,060,906 - Pf6N2E2_5266 0.63 +0.6 6,060,942 + Pf6N2E2_5266 0.66 -0.6 6,060,942 + Pf6N2E2_5266 0.66 -0.1 6,060,943 - Pf6N2E2_5266 0.66 +0.1 6,060,943 - Pf6N2E2_5266 0.66 +0.0 6,060,943 - Pf6N2E2_5266 0.66 +0.1 6,060,982 + Pf6N2E2_5266 0.69 -0.6
Or see this region's nucleotide sequence