Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5022 and Pf6N2E2_5023 are separated by 28 nucleotides Pf6N2E2_5023 and Pf6N2E2_5024 are separated by 17 nucleotides Pf6N2E2_5024 and Pf6N2E2_5025 are separated by 439 nucleotides
Pf6N2E2_5022 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 5,787,438 to 5,788,721
_5022
Pf6N2E2_5023 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 5,788,750 to 5,789,376
_5023
Pf6N2E2_5024 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 5,789,394 to 5,790,122
_5024
Pf6N2E2_5025 - Sensor histidine kinase, at 5,790,562 to 5,792,946
_5025
Position (kb) 5789
5790
5791 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5788.752 kb on - strand at 5788.752 kb on - strand at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.358 kb on - strand at 5789.369 kb on - strand at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.573 kb on - strand, within Pf6N2E2_5024 at 5789.598 kb on - strand, within Pf6N2E2_5024 at 5790.111 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.205 kb on - strand at 5790.237 kb on + strand at 5790.237 kb on + strand at 5790.241 kb on - strand at 5790.241 kb on - strand at 5790.241 kb on - strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.255 kb on + strand at 5790.255 kb on + strand at 5790.255 kb on + strand at 5790.256 kb on - strand at 5790.256 kb on - strand at 5790.256 kb on - strand at 5790.266 kb on + strand at 5790.266 kb on + strand at 5790.267 kb on - strand at 5790.267 kb on - strand at 5790.283 kb on + strand at 5790.284 kb on - strand at 5790.284 kb on - strand at 5790.346 kb on + strand at 5790.375 kb on - strand at 5790.457 kb on + strand at 5790.457 kb on + strand at 5790.462 kb on + strand at 5790.462 kb on + strand at 5790.462 kb on + strand at 5790.463 kb on - strand at 5790.463 kb on - strand at 5790.463 kb on - strand at 5790.481 kb on + strand at 5790.488 kb on + strand at 5790.489 kb on - strand at 5790.500 kb on + strand at 5790.501 kb on - strand at 5790.506 kb on + strand at 5790.559 kb on + strand at 5790.559 kb on + strand at 5790.560 kb on - strand at 5790.560 kb on - strand at 5790.560 kb on - strand at 5790.568 kb on + strand at 5790.569 kb on - strand at 5790.569 kb on - strand at 5790.693 kb on + strand at 5790.693 kb on + strand at 5790.693 kb on + strand at 5790.693 kb on + strand at 5790.694 kb on - strand at 5790.834 kb on + strand, within Pf6N2E2_5025 at 5790.835 kb on - strand, within Pf6N2E2_5025 at 5790.883 kb on + strand, within Pf6N2E2_5025 at 5790.883 kb on + strand, within Pf6N2E2_5025 at 5790.883 kb on + strand, within Pf6N2E2_5025 at 5790.883 kb on + strand, within Pf6N2E2_5025 at 5790.884 kb on - strand, within Pf6N2E2_5025 at 5790.884 kb on - strand, within Pf6N2E2_5025 at 5790.884 kb on - strand, within Pf6N2E2_5025 at 5790.973 kb on + strand, within Pf6N2E2_5025 at 5790.973 kb on + strand, within Pf6N2E2_5025 at 5790.974 kb on - strand, within Pf6N2E2_5025 at 5790.974 kb on - strand, within Pf6N2E2_5025 at 5790.974 kb on - strand, within Pf6N2E2_5025 at 5790.974 kb on - strand, within Pf6N2E2_5025 at 5790.974 kb on - strand, within Pf6N2E2_5025 at 5791.018 kb on + strand, within Pf6N2E2_5025 at 5791.018 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.108 kb on + strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.109 kb on - strand, within Pf6N2E2_5025 at 5791.112 kb on + strand, within Pf6N2E2_5025 at 5791.112 kb on + strand, within Pf6N2E2_5025 at 5791.112 kb on + strand, within Pf6N2E2_5025 at 5791.113 kb on - strand, within Pf6N2E2_5025 at 5791.113 kb on - strand, within Pf6N2E2_5025
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,788,752 - -0.8 5,788,752 - -0.2 5,789,010 - Pf6N2E2_5023 0.41 -0.8 5,789,010 - Pf6N2E2_5023 0.41 -0.7 5,789,010 - Pf6N2E2_5023 0.41 +0.5 5,789,309 - Pf6N2E2_5023 0.89 -1.3 5,789,309 - Pf6N2E2_5023 0.89 -0.8 5,789,309 - Pf6N2E2_5023 0.89 +0.2 5,789,309 - Pf6N2E2_5023 0.89 +0.1 5,789,313 - Pf6N2E2_5023 0.90 +0.3 5,789,313 - Pf6N2E2_5023 0.90 +0.9 5,789,313 - Pf6N2E2_5023 0.90 -0.4 5,789,313 - Pf6N2E2_5023 0.90 -0.6 5,789,358 - +0.7 5,789,369 - -0.8 5,789,480 - Pf6N2E2_5024 0.12 -1.1 5,789,480 - Pf6N2E2_5024 0.12 +0.1 5,789,573 - Pf6N2E2_5024 0.25 -0.6 5,789,598 - Pf6N2E2_5024 0.28 -0.9 5,790,111 - -0.1 5,790,182 - +0.1 5,790,182 - +0.8 5,790,182 - -0.3 5,790,182 - +0.9 5,790,182 - +0.3 5,790,182 - +0.6 5,790,186 - -1.6 5,790,186 - -1.1 5,790,186 - +0.6 5,790,186 - -0.1 5,790,205 - +1.2 5,790,237 + +0.2 5,790,237 + +0.3 5,790,241 - +0.4 5,790,241 - +0.2 5,790,241 - -0.6 5,790,253 + +0.2 5,790,253 + -0.7 5,790,253 + +1.6 5,790,253 + +0.0 5,790,253 + +0.7 5,790,253 + -0.6 5,790,254 - -1.0 5,790,254 - +1.0 5,790,254 - -2.1 5,790,254 - +0.0 5,790,254 - +0.5 5,790,254 - -0.1 5,790,254 - +0.7 5,790,254 - -0.0 5,790,254 - +0.1 5,790,255 + -0.1 5,790,255 + +0.5 5,790,255 + -0.8 5,790,256 - -0.3 5,790,256 - +0.2 5,790,256 - +0.1 5,790,266 + +1.7 5,790,266 + +0.8 5,790,267 - -0.3 5,790,267 - -0.6 5,790,283 + -0.8 5,790,284 - -0.5 5,790,284 - -0.1 5,790,346 + +0.9 5,790,375 - -0.3 5,790,457 + +0.0 5,790,457 + +0.4 5,790,462 + -1.4 5,790,462 + +1.6 5,790,462 + +0.5 5,790,463 - +0.8 5,790,463 - +0.8 5,790,463 - -0.7 5,790,481 + +0.5 5,790,488 + +0.4 5,790,489 - +0.3 5,790,500 + +0.4 5,790,501 - -0.3 5,790,506 + +0.5 5,790,559 + -0.0 5,790,559 + -0.3 5,790,560 - -0.5 5,790,560 - -0.3 5,790,560 - +0.6 5,790,568 + +0.6 5,790,569 - +0.9 5,790,569 - +0.3 5,790,693 + -0.3 5,790,693 + +0.1 5,790,693 + +0.4 5,790,693 + -0.0 5,790,694 - +0.4 5,790,834 + Pf6N2E2_5025 0.11 +0.5 5,790,835 - Pf6N2E2_5025 0.11 +1.2 5,790,883 + Pf6N2E2_5025 0.13 -0.2 5,790,883 + Pf6N2E2_5025 0.13 -1.8 5,790,883 + Pf6N2E2_5025 0.13 -0.3 5,790,883 + Pf6N2E2_5025 0.13 -0.3 5,790,884 - Pf6N2E2_5025 0.14 -1.1 5,790,884 - Pf6N2E2_5025 0.14 +0.2 5,790,884 - Pf6N2E2_5025 0.14 +1.1 5,790,973 + Pf6N2E2_5025 0.17 -0.2 5,790,973 + Pf6N2E2_5025 0.17 +0.4 5,790,974 - Pf6N2E2_5025 0.17 +0.2 5,790,974 - Pf6N2E2_5025 0.17 -0.4 5,790,974 - Pf6N2E2_5025 0.17 -0.4 5,790,974 - Pf6N2E2_5025 0.17 -0.1 5,790,974 - Pf6N2E2_5025 0.17 -0.0 5,791,018 + Pf6N2E2_5025 0.19 +0.8 5,791,018 + Pf6N2E2_5025 0.19 +1.5 5,791,108 + Pf6N2E2_5025 0.23 +1.0 5,791,108 + Pf6N2E2_5025 0.23 +0.0 5,791,108 + Pf6N2E2_5025 0.23 -0.3 5,791,108 + Pf6N2E2_5025 0.23 +0.1 5,791,108 + Pf6N2E2_5025 0.23 +0.6 5,791,108 + Pf6N2E2_5025 0.23 +0.6 5,791,108 + Pf6N2E2_5025 0.23 -0.5 5,791,108 + Pf6N2E2_5025 0.23 -0.1 5,791,108 + Pf6N2E2_5025 0.23 +0.1 5,791,108 + Pf6N2E2_5025 0.23 +0.6 5,791,108 + Pf6N2E2_5025 0.23 +0.3 5,791,108 + Pf6N2E2_5025 0.23 -2.1 5,791,108 + Pf6N2E2_5025 0.23 +0.4 5,791,108 + Pf6N2E2_5025 0.23 -1.8 5,791,109 - Pf6N2E2_5025 0.23 -2.5 5,791,109 - Pf6N2E2_5025 0.23 -0.7 5,791,109 - Pf6N2E2_5025 0.23 +0.0 5,791,109 - Pf6N2E2_5025 0.23 +0.6 5,791,109 - Pf6N2E2_5025 0.23 -0.7 5,791,109 - Pf6N2E2_5025 0.23 -0.3 5,791,109 - Pf6N2E2_5025 0.23 +0.1 5,791,109 - Pf6N2E2_5025 0.23 -1.4 5,791,109 - Pf6N2E2_5025 0.23 -0.1 5,791,109 - Pf6N2E2_5025 0.23 -0.5 5,791,109 - Pf6N2E2_5025 0.23 -0.3 5,791,109 - Pf6N2E2_5025 0.23 +0.1 5,791,109 - Pf6N2E2_5025 0.23 +0.1 5,791,109 - Pf6N2E2_5025 0.23 -0.4 5,791,109 - Pf6N2E2_5025 0.23 -0.9 5,791,109 - Pf6N2E2_5025 0.23 -0.2 5,791,109 - Pf6N2E2_5025 0.23 +0.6 5,791,109 - Pf6N2E2_5025 0.23 -0.3 5,791,109 - Pf6N2E2_5025 0.23 -0.6 5,791,109 - Pf6N2E2_5025 0.23 -1.2 5,791,109 - Pf6N2E2_5025 0.23 +1.5 5,791,112 + Pf6N2E2_5025 0.23 -0.0 5,791,112 + Pf6N2E2_5025 0.23 -0.5 5,791,112 + Pf6N2E2_5025 0.23 +2.2 5,791,113 - Pf6N2E2_5025 0.23 +0.4 5,791,113 - Pf6N2E2_5025 0.23 +0.1
Or see this region's nucleotide sequence