Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_468 and Pf6N2E2_469 are separated by 251 nucleotides Pf6N2E2_469 and Pf6N2E2_470 overlap by 4 nucleotides Pf6N2E2_470 and Pf6N2E2_471 overlap by 1 nucleotides Pf6N2E2_471 and Pf6N2E2_472 are separated by 288 nucleotides Pf6N2E2_472 and Pf6N2E2_473 are separated by 13 nucleotides
Pf6N2E2_468 - DNA alkylation repair enzyme, at 534,448 to 535,551
_468
Pf6N2E2_469 - lipoprotein, putative, at 535,803 to 536,111
_469
Pf6N2E2_470 - hypothetical protein, at 536,108 to 536,431
_470
Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492
_471
Pf6N2E2_472 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 537,781 to 538,122
_472
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Position (kb) 536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 535.460 kb on + strand at 535.581 kb on + strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.582 kb on - strand at 535.584 kb on - strand at 535.584 kb on - strand at 535.740 kb on - strand at 536.052 kb on + strand, within Pf6N2E2_469 at 536.052 kb on + strand, within Pf6N2E2_469 at 536.053 kb on - strand, within Pf6N2E2_469 at 536.110 kb on - strand at 536.110 kb on - strand at 536.591 kb on + strand at 536.648 kb on + strand, within Pf6N2E2_471 at 536.648 kb on + strand, within Pf6N2E2_471 at 536.648 kb on + strand, within Pf6N2E2_471 at 536.649 kb on - strand, within Pf6N2E2_471 at 536.649 kb on - strand, within Pf6N2E2_471 at 536.873 kb on - strand, within Pf6N2E2_471 at 536.942 kb on + strand, within Pf6N2E2_471 at 536.943 kb on - strand, within Pf6N2E2_471 at 537.095 kb on + strand, within Pf6N2E2_471 at 537.096 kb on - strand, within Pf6N2E2_471 at 537.127 kb on - strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.452 kb on + strand at 537.452 kb on + strand at 537.452 kb on + strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.505 kb on - strand at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 538.031 kb on - strand, within Pf6N2E2_472 at 538.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 535,460 + -1.4 535,581 + +0.3 535,582 - +1.4 535,582 - +1.8 535,582 - +0.4 535,582 - +0.2 535,584 - +0.8 535,584 - +0.5 535,740 - -2.8 536,052 + Pf6N2E2_469 0.81 +1.8 536,052 + Pf6N2E2_469 0.81 -1.1 536,053 - Pf6N2E2_469 0.81 +0.9 536,110 - -1.5 536,110 - +0.5 536,591 + +1.5 536,648 + Pf6N2E2_471 0.20 -0.6 536,648 + Pf6N2E2_471 0.20 -1.2 536,648 + Pf6N2E2_471 0.20 -2.8 536,649 - Pf6N2E2_471 0.21 -1.6 536,649 - Pf6N2E2_471 0.21 -0.2 536,873 - Pf6N2E2_471 0.42 -0.9 536,942 + Pf6N2E2_471 0.48 -1.0 536,943 - Pf6N2E2_471 0.48 -0.4 537,095 + Pf6N2E2_471 0.63 -1.3 537,096 - Pf6N2E2_471 0.63 +0.7 537,127 - Pf6N2E2_471 0.66 +0.7 537,170 + Pf6N2E2_471 0.70 -0.9 537,170 + Pf6N2E2_471 0.70 -1.8 537,170 + Pf6N2E2_471 0.70 -1.2 537,170 + Pf6N2E2_471 0.70 -1.6 537,171 - Pf6N2E2_471 0.70 +0.4 537,171 - Pf6N2E2_471 0.70 -0.7 537,452 + -0.6 537,452 + -0.7 537,452 + -0.8 537,453 - -0.5 537,453 - -0.5 537,453 - -1.7 537,453 - -1.5 537,505 - +1.5 537,833 + Pf6N2E2_472 0.15 +1.7 537,833 + Pf6N2E2_472 0.15 -1.1 537,833 + Pf6N2E2_472 0.15 +0.9 537,904 + Pf6N2E2_472 0.36 +0.5 537,904 + Pf6N2E2_472 0.36 -0.1 537,905 - Pf6N2E2_472 0.36 -1.7 537,905 - Pf6N2E2_472 0.36 +0.0 538,031 - Pf6N2E2_472 0.73 +1.0 538,212 - -0.1
Or see this region's nucleotide sequence