Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_330 and Pf6N2E2_331 are separated by 265 nucleotides Pf6N2E2_331 and Pf6N2E2_332 are separated by 23 nucleotides Pf6N2E2_332 and Pf6N2E2_333 are separated by 17 nucleotides Pf6N2E2_333 and Pf6N2E2_334 are separated by 167 nucleotides
Pf6N2E2_330 - Mesenchymal stem cell protein DSCD75, at 398,698 to 399,249
_330
Pf6N2E2_331 - Putative translation initiation inhibitor, yjgF family, at 399,515 to 399,946
_331
Pf6N2E2_332 - putative nucleoside-diphosphate-sugar epimerase, at 399,970 to 400,848
_332
Pf6N2E2_333 - hypothetical protein, at 400,866 to 401,549
_333
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Position (kb) 399
400
401 Strain fitness (log2 ratio) -2
-1
0
1 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.075 kb on - strand, within Pf6N2E2_330 at 399.107 kb on + strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.283 kb on + strand at 399.284 kb on - strand at 399.307 kb on - strand at 399.392 kb on + strand at 399.494 kb on - strand at 399.624 kb on + strand, within Pf6N2E2_331 at 399.624 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.938 kb on - strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.075 kb on + strand, within Pf6N2E2_332 at 400.232 kb on + strand, within Pf6N2E2_332 at 400.415 kb on + strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.419 kb on - strand, within Pf6N2E2_332 at 400.501 kb on - strand, within Pf6N2E2_332 at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.854 kb on + strand at 400.854 kb on + strand at 401.047 kb on + strand, within Pf6N2E2_333 at 401.047 kb on + strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.143 kb on + strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.192 kb on - strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.275 kb on - strand, within Pf6N2E2_333 at 401.438 kb on - strand, within Pf6N2E2_333 at 401.501 kb on - strand at 401.501 kb on - strand at 401.549 kb on + strand at 401.649 kb on + strand at 401.649 kb on + strand at 401.650 kb on - strand at 401.838 kb on + strand, within Pf6N2E2_334 at 401.841 kb on + strand, within Pf6N2E2_334
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 399,074 + Pf6N2E2_330 0.68 +0.0 399,074 + Pf6N2E2_330 0.68 -1.0 399,075 - Pf6N2E2_330 0.68 +0.6 399,107 + Pf6N2E2_330 0.74 -0.2 399,108 - Pf6N2E2_330 0.74 -0.6 399,108 - Pf6N2E2_330 0.74 +0.2 399,283 + +0.1 399,284 - +0.5 399,307 - -0.3 399,392 + -0.3 399,494 - -1.0 399,624 + Pf6N2E2_331 0.25 -1.6 399,624 + Pf6N2E2_331 0.25 +0.6 399,666 + Pf6N2E2_331 0.35 +0.4 399,666 + Pf6N2E2_331 0.35 -0.8 399,666 + Pf6N2E2_331 0.35 +0.3 399,938 - +1.6 400,016 + +1.4 400,016 + -0.9 400,016 + +0.3 400,016 + +0.8 400,016 + -0.1 400,017 - +1.4 400,017 - -1.3 400,017 - -2.5 400,017 - +0.2 400,017 - +0.8 400,075 + Pf6N2E2_332 0.12 -1.0 400,232 + Pf6N2E2_332 0.30 -1.3 400,415 + Pf6N2E2_332 0.51 -0.9 400,416 - Pf6N2E2_332 0.51 -1.9 400,416 - Pf6N2E2_332 0.51 -1.2 400,416 - Pf6N2E2_332 0.51 -1.9 400,416 - Pf6N2E2_332 0.51 +0.7 400,416 - Pf6N2E2_332 0.51 -0.0 400,416 - Pf6N2E2_332 0.51 -1.1 400,419 - Pf6N2E2_332 0.51 -0.7 400,501 - Pf6N2E2_332 0.60 -0.0 400,844 + +1.4 400,844 + +0.3 400,844 + +0.9 400,844 + +0.2 400,844 + -0.7 400,845 - -0.6 400,845 - -1.3 400,845 - -0.0 400,845 - +0.7 400,845 - -1.0 400,854 + -0.0 400,854 + -1.7 401,047 + Pf6N2E2_333 0.26 -1.1 401,047 + Pf6N2E2_333 0.26 -0.4 401,048 - Pf6N2E2_333 0.27 +0.3 401,048 - Pf6N2E2_333 0.27 +0.6 401,048 - Pf6N2E2_333 0.27 +0.3 401,048 - Pf6N2E2_333 0.27 -1.2 401,143 + Pf6N2E2_333 0.40 -0.6 401,144 - Pf6N2E2_333 0.41 -1.1 401,144 - Pf6N2E2_333 0.41 +0.6 401,144 - Pf6N2E2_333 0.41 -0.1 401,191 + Pf6N2E2_333 0.48 -0.6 401,191 + Pf6N2E2_333 0.48 +1.0 401,192 - Pf6N2E2_333 0.48 -0.1 401,274 + Pf6N2E2_333 0.60 +1.3 401,274 + Pf6N2E2_333 0.60 +1.0 401,274 + Pf6N2E2_333 0.60 +0.1 401,275 - Pf6N2E2_333 0.60 -1.7 401,438 - Pf6N2E2_333 0.84 -0.8 401,501 - -0.0 401,501 - -0.1 401,549 + +1.4 401,649 + +0.5 401,649 + -0.0 401,650 - +0.4 401,838 + Pf6N2E2_334 0.13 -0.0 401,841 + Pf6N2E2_334 0.13 +0.5
Or see this region's nucleotide sequence