Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1668 and Pf6N2E2_1669 are separated by 134 nucleotides Pf6N2E2_1669 and Pf6N2E2_1670 are separated by 62 nucleotides Pf6N2E2_1670 and Pf6N2E2_1671 are separated by 24 nucleotides Pf6N2E2_1671 and Pf6N2E2_1672 are separated by 148 nucleotides
Pf6N2E2_1668 - Xylonate dehydratase (EC 4.2.1.82) (from data) , at 1,974,601 to 1,976,385
_1668
Pf6N2E2_1669 - Probable tautomerase, at 1,976,520 to 1,976,738
_1669
Pf6N2E2_1670 - conserved membrane protein, at 1,976,801 to 1,977,310
_1670
Pf6N2E2_1671 - Transcriptional regulator, TetR family, at 1,977,335 to 1,978,012
_1671
Pf6N2E2_1672 - hypothetical protein, at 1,978,161 to 1,979,042
_1672
Position (kb)
1977
1978
1979 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.442 kb on - strand at 1976.501 kb on + strand at 1976.535 kb on + strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.613 kb on + strand, within Pf6N2E2_1669 at 1976.744 kb on - strand at 1976.750 kb on + strand at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1977.182 kb on + strand, within Pf6N2E2_1670 at 1977.183 kb on - strand, within Pf6N2E2_1670 at 1977.211 kb on - strand, within Pf6N2E2_1670 at 1977.258 kb on - strand, within Pf6N2E2_1670 at 1977.284 kb on + strand at 1977.284 kb on + strand at 1977.390 kb on + strand at 1977.390 kb on + strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.471 kb on - strand at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.472 kb on + strand, within Pf6N2E2_1671 at 1977.605 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.717 kb on - strand, within Pf6N2E2_1671 at 1977.828 kb on - strand, within Pf6N2E2_1671 at 1977.837 kb on + strand, within Pf6N2E2_1671 at 1978.027 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.035 kb on - strand at 1978.063 kb on + strand at 1978.063 kb on + strand at 1978.067 kb on + strand at 1978.067 kb on + strand at 1978.068 kb on - strand at 1978.068 kb on - strand at 1978.092 kb on + strand at 1978.093 kb on - strand at 1978.143 kb on + strand at 1978.143 kb on + strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.154 kb on + strand at 1978.155 kb on - strand at 1978.155 kb on - strand at 1978.174 kb on + strand at 1978.191 kb on + strand at 1978.254 kb on + strand, within Pf6N2E2_1672 at 1978.408 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.521 kb on + strand, within Pf6N2E2_1672 at 1978.540 kb on - strand, within Pf6N2E2_1672 at 1978.614 kb on + strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.622 kb on + strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.732 kb on + strand, within Pf6N2E2_1672 at 1978.733 kb on - strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.830 kb on + strand, within Pf6N2E2_1672 at 1978.831 kb on - strand, within Pf6N2E2_1672 at 1978.906 kb on + strand, within Pf6N2E2_1672 at 1978.907 kb on - strand, within Pf6N2E2_1672
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,976,441 + +2.1 1,976,441 + +0.4 1,976,441 + +0.5 1,976,442 - +0.6 1,976,501 + -0.8 1,976,535 + +1.1 1,976,536 - +0.2 1,976,536 - +0.8 1,976,536 - -0.0 1,976,613 + Pf6N2E2_1669 0.42 +1.3 1,976,744 - -0.8 1,976,750 + +1.4 1,976,922 - Pf6N2E2_1670 0.24 -1.0 1,976,922 - Pf6N2E2_1670 0.24 +0.4 1,977,182 + Pf6N2E2_1670 0.75 +1.2 1,977,183 - Pf6N2E2_1670 0.75 +0.2 1,977,211 - Pf6N2E2_1670 0.80 -0.2 1,977,258 - Pf6N2E2_1670 0.90 +0.5 1,977,284 + +0.7 1,977,284 + -0.6 1,977,390 + -1.0 1,977,390 + +0.1 1,977,391 - +1.3 1,977,391 - +1.4 1,977,391 - -0.9 1,977,391 - -0.3 1,977,471 - +0.4 1,977,471 - Pf6N2E2_1671 0.20 -0.7 1,977,471 - Pf6N2E2_1671 0.20 -2.0 1,977,472 + Pf6N2E2_1671 0.20 -2.2 1,977,605 + Pf6N2E2_1671 0.40 -1.1 1,977,716 + Pf6N2E2_1671 0.56 +0.4 1,977,716 + Pf6N2E2_1671 0.56 -0.9 1,977,717 - Pf6N2E2_1671 0.56 +0.6 1,977,828 - Pf6N2E2_1671 0.73 -1.7 1,977,837 + Pf6N2E2_1671 0.74 -0.2 1,978,027 - +0.4 1,978,029 - -0.9 1,978,029 - +0.4 1,978,029 - +2.0 1,978,035 - -0.3 1,978,063 + +0.2 1,978,063 + -0.2 1,978,067 + -0.9 1,978,067 + -1.7 1,978,068 - -0.3 1,978,068 - -1.7 1,978,092 + +0.8 1,978,093 - -0.7 1,978,143 + +0.6 1,978,143 + -0.7 1,978,144 - -0.2 1,978,144 - +1.1 1,978,144 - +1.4 1,978,154 + +0.8 1,978,155 - +1.4 1,978,155 - -0.7 1,978,174 + -0.2 1,978,191 + -0.2 1,978,254 + Pf6N2E2_1672 0.11 -0.6 1,978,408 - Pf6N2E2_1672 0.28 -0.6 1,978,435 - Pf6N2E2_1672 0.31 -0.6 1,978,435 - Pf6N2E2_1672 0.31 -0.0 1,978,435 - Pf6N2E2_1672 0.31 +0.6 1,978,435 - Pf6N2E2_1672 0.31 -0.3 1,978,521 + Pf6N2E2_1672 0.41 -1.6 1,978,540 - Pf6N2E2_1672 0.43 -0.4 1,978,614 + Pf6N2E2_1672 0.51 +0.8 1,978,615 - Pf6N2E2_1672 0.51 +0.4 1,978,615 - Pf6N2E2_1672 0.51 +0.7 1,978,615 - Pf6N2E2_1672 0.51 +0.1 1,978,615 - Pf6N2E2_1672 0.51 -2.0 1,978,622 + Pf6N2E2_1672 0.52 -0.3 1,978,623 - Pf6N2E2_1672 0.52 +0.6 1,978,623 - Pf6N2E2_1672 0.52 -0.1 1,978,623 - Pf6N2E2_1672 0.52 +0.8 1,978,623 - Pf6N2E2_1672 0.52 -0.2 1,978,623 - Pf6N2E2_1672 0.52 +0.5 1,978,623 - Pf6N2E2_1672 0.52 +0.3 1,978,642 - Pf6N2E2_1672 0.55 -0.7 1,978,642 - Pf6N2E2_1672 0.55 -0.2 1,978,732 + Pf6N2E2_1672 0.65 +0.5 1,978,733 - Pf6N2E2_1672 0.65 -0.3 1,978,780 + Pf6N2E2_1672 0.70 +0.6 1,978,780 + Pf6N2E2_1672 0.70 -1.1 1,978,780 + Pf6N2E2_1672 0.70 -0.2 1,978,830 + Pf6N2E2_1672 0.76 +0.9 1,978,831 - Pf6N2E2_1672 0.76 -1.0 1,978,906 + Pf6N2E2_1672 0.84 -0.9 1,978,907 - Pf6N2E2_1672 0.85 +1.3
Or see this region's nucleotide sequence