Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1382 and Pf6N2E2_1383 are separated by 408 nucleotides Pf6N2E2_1383 and Pf6N2E2_1384 are separated by 386 nucleotides
Pf6N2E2_1382 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,622,393 to 1,624,168
_1382
Pf6N2E2_1383 - YD repeat protein, at 1,624,577 to 1,625,677
_1383
Pf6N2E2_1384 - SoxH protein, homolog, at 1,626,064 to 1,626,993
_1384
Position (kb)
1624
1625
1626 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1623.811 kb on - strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.862 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1624.002 kb on + strand at 1624.003 kb on - strand at 1624.195 kb on + strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.359 kb on - strand at 1624.441 kb on - strand at 1624.441 kb on - strand at 1624.467 kb on - strand at 1624.472 kb on - strand at 1624.505 kb on + strand at 1624.506 kb on - strand at 1624.506 kb on - strand at 1624.660 kb on - strand at 1624.673 kb on - strand at 1624.692 kb on - strand, within Pf6N2E2_1383 at 1624.696 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.833 kb on - strand, within Pf6N2E2_1383 at 1624.900 kb on - strand, within Pf6N2E2_1383 at 1625.015 kb on - strand, within Pf6N2E2_1383 at 1625.170 kb on + strand, within Pf6N2E2_1383 at 1625.194 kb on - strand, within Pf6N2E2_1383 at 1625.298 kb on + strand, within Pf6N2E2_1383 at 1625.477 kb on - strand, within Pf6N2E2_1383 at 1625.501 kb on + strand, within Pf6N2E2_1383 at 1625.515 kb on - strand, within Pf6N2E2_1383 at 1625.608 kb on + strand at 1625.608 kb on + strand at 1625.609 kb on - strand at 1625.677 kb on + strand at 1625.677 kb on + strand at 1625.678 kb on - strand at 1625.689 kb on + strand at 1625.689 kb on + strand at 1625.690 kb on - strand at 1625.690 kb on - strand at 1625.690 kb on - strand at 1625.693 kb on - strand at 1625.693 kb on - strand at 1625.745 kb on - strand at 1625.899 kb on + strand at 1625.997 kb on + strand at 1625.998 kb on - strand at 1625.998 kb on - strand at 1626.141 kb on - strand at 1626.254 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.405 kb on - strand, within Pf6N2E2_1384 at 1626.405 kb on - strand, within Pf6N2E2_1384 at 1626.590 kb on + strand, within Pf6N2E2_1384 at 1626.591 kb on - strand, within Pf6N2E2_1384 at 1626.591 kb on - strand, within Pf6N2E2_1384
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,623,811 - Pf6N2E2_1382 0.80 -1.2 1,623,861 + Pf6N2E2_1382 0.83 -0.5 1,623,861 + Pf6N2E2_1382 0.83 +0.7 1,623,862 - Pf6N2E2_1382 0.83 -0.5 1,623,877 - Pf6N2E2_1382 0.84 +0.0 1,623,877 - Pf6N2E2_1382 0.84 +1.3 1,623,877 - Pf6N2E2_1382 0.84 -0.1 1,623,877 - Pf6N2E2_1382 0.84 +1.0 1,624,002 + -1.1 1,624,003 - -0.3 1,624,195 + +0.3 1,624,288 - -2.1 1,624,288 - -1.3 1,624,288 - -0.7 1,624,288 - -2.8 1,624,359 - +0.3 1,624,441 - -0.0 1,624,441 - +1.3 1,624,467 - +0.3 1,624,472 - +0.6 1,624,505 + +0.1 1,624,506 - +1.9 1,624,506 - -0.3 1,624,660 - -0.6 1,624,673 - -0.5 1,624,692 - Pf6N2E2_1383 0.10 -0.0 1,624,696 - Pf6N2E2_1383 0.11 +0.7 1,624,796 - Pf6N2E2_1383 0.20 -1.9 1,624,796 - Pf6N2E2_1383 0.20 +0.1 1,624,833 - Pf6N2E2_1383 0.23 -1.3 1,624,900 - Pf6N2E2_1383 0.29 -0.4 1,625,015 - Pf6N2E2_1383 0.40 +1.7 1,625,170 + Pf6N2E2_1383 0.54 -0.8 1,625,194 - Pf6N2E2_1383 0.56 +1.1 1,625,298 + Pf6N2E2_1383 0.65 -1.4 1,625,477 - Pf6N2E2_1383 0.82 -1.7 1,625,501 + Pf6N2E2_1383 0.84 -1.4 1,625,515 - Pf6N2E2_1383 0.85 -0.6 1,625,608 + +0.7 1,625,608 + +0.0 1,625,609 - +1.3 1,625,677 + +0.4 1,625,677 + +0.5 1,625,678 - -0.8 1,625,689 + +1.5 1,625,689 + +1.0 1,625,690 - +0.4 1,625,690 - +1.0 1,625,690 - -1.3 1,625,693 - -1.5 1,625,693 - +0.0 1,625,745 - +0.4 1,625,899 + -0.3 1,625,997 + +0.4 1,625,998 - +2.3 1,625,998 - +0.0 1,626,141 - -1.1 1,626,254 + Pf6N2E2_1384 0.20 -0.1 1,626,404 + Pf6N2E2_1384 0.37 +0.2 1,626,404 + Pf6N2E2_1384 0.37 -1.3 1,626,404 + Pf6N2E2_1384 0.37 +1.6 1,626,405 - Pf6N2E2_1384 0.37 -0.3 1,626,405 - Pf6N2E2_1384 0.37 +1.9 1,626,590 + Pf6N2E2_1384 0.57 +0.5 1,626,591 - Pf6N2E2_1384 0.57 -0.1 1,626,591 - Pf6N2E2_1384 0.57 +0.5
Or see this region's nucleotide sequence