Experiment: Succinic Acid; Light intensity 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1941 and Rru_A1942 overlap by 19 nucleotides Rru_A1942 and Rru_A1943 are separated by 13 nucleotides Rru_A1943 and Rru_A1944 are separated by 111 nucleotides Rru_A1944 and Rru_A1945 are separated by 156 nucleotides
Rru_A1941: Rru_A1941 - acyl-CoA carboxylase alpha chain (NCBI), at 2,248,388 to 2,250,448
_A1941
Rru_A1942: Rru_A1942 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,250,430 to 2,251,257
_A1942
Rru_A1943: Rru_A1943 - Propionyl-CoA carboxylase (NCBI), at 2,251,271 to 2,252,878
_A1943
Rru_A1944: Rru_A1944 - DSBA oxidoreductase (NCBI), at 2,252,990 to 2,253,592
_A1944
Rru_A1945: Rru_A1945 - Short-chain dehydrogenase/reductase SDR (NCBI), at 2,253,749 to 2,254,432
_A1945
Position (kb)
2251
2252
2253 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2250.815 kb on + strand, within Rru_A1942 at 2250.815 kb on + strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.402 kb on - strand at 2251.404 kb on + strand at 2251.405 kb on - strand at 2251.405 kb on - strand at 2251.425 kb on + strand at 2251.426 kb on - strand at 2251.593 kb on + strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.137 kb on - strand, within Rru_A1943 at 2252.298 kb on + strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.377 kb on - strand, within Rru_A1943 at 2252.457 kb on + strand, within Rru_A1943 at 2252.527 kb on - strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.907 kb on - strand at 2252.991 kb on + strand at 2253.005 kb on - strand at 2253.017 kb on - strand at 2253.243 kb on + strand, within Rru_A1944 at 2253.243 kb on + strand, within Rru_A1944 at 2253.243 kb on + strand, within Rru_A1944 at 2253.267 kb on + strand, within Rru_A1944 at 2253.353 kb on + strand, within Rru_A1944 at 2253.353 kb on + strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.408 kb on + strand, within Rru_A1944 at 2253.408 kb on + strand, within Rru_A1944 at 2253.409 kb on - strand, within Rru_A1944 at 2253.409 kb on - strand, within Rru_A1944 at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.549 kb on + strand at 2253.550 kb on - strand at 2253.570 kb on + strand at 2253.645 kb on - strand at 2253.645 kb on - strand at 2253.645 kb on - strand at 2253.735 kb on - strand at 2253.750 kb on + strand at 2253.774 kb on + strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 150 uM remove 2,250,815 + Rru_A1942 0.46 -1.8 2,250,815 + Rru_A1942 0.46 -1.6 2,250,816 - Rru_A1942 0.47 -0.7 2,250,816 - Rru_A1942 0.47 -2.7 2,250,816 - Rru_A1942 0.47 -2.5 2,250,816 - Rru_A1942 0.47 -1.0 2,250,816 - Rru_A1942 0.47 +0.0 2,250,816 - Rru_A1942 0.47 -3.4 2,250,929 + Rru_A1942 0.60 +0.0 2,250,929 + Rru_A1942 0.60 -1.5 2,251,401 + +0.0 2,251,401 + -2.5 2,251,401 + +0.0 2,251,401 + -1.7 2,251,402 - -2.0 2,251,404 + -1.9 2,251,405 - +1.0 2,251,405 - -2.8 2,251,425 + +0.8 2,251,426 - +0.0 2,251,593 + Rru_A1943 0.20 -0.5 2,251,615 - Rru_A1943 0.21 -1.0 2,251,615 - Rru_A1943 0.21 +0.0 2,251,911 + Rru_A1943 0.40 -3.8 2,251,911 + Rru_A1943 0.40 -2.3 2,251,977 + Rru_A1943 0.44 +0.0 2,251,977 + Rru_A1943 0.44 +0.0 2,251,978 - Rru_A1943 0.44 +1.0 2,251,978 - Rru_A1943 0.44 -1.9 2,252,028 + Rru_A1943 0.47 -1.1 2,252,028 + Rru_A1943 0.47 +0.0 2,252,028 + Rru_A1943 0.47 -4.1 2,252,029 - Rru_A1943 0.47 -2.4 2,252,029 - Rru_A1943 0.47 -0.4 2,252,029 - Rru_A1943 0.47 +0.0 2,252,029 - Rru_A1943 0.47 +0.0 2,252,136 + Rru_A1943 0.54 +0.0 2,252,136 + Rru_A1943 0.54 +0.0 2,252,137 - Rru_A1943 0.54 +0.0 2,252,298 + Rru_A1943 0.64 -1.3 2,252,299 - Rru_A1943 0.64 -3.1 2,252,299 - Rru_A1943 0.64 -4.0 2,252,299 - Rru_A1943 0.64 -1.0 2,252,299 - Rru_A1943 0.64 +0.0 2,252,377 - Rru_A1943 0.69 -1.0 2,252,457 + Rru_A1943 0.74 -2.0 2,252,527 - Rru_A1943 0.78 -1.3 2,252,529 + Rru_A1943 0.78 +0.0 2,252,529 + Rru_A1943 0.78 +0.0 2,252,529 + Rru_A1943 0.78 +0.0 2,252,529 + Rru_A1943 0.78 -2.9 2,252,529 + Rru_A1943 0.78 -2.1 2,252,529 + Rru_A1943 0.78 -1.4 2,252,529 + Rru_A1943 0.78 -2.5 2,252,529 + Rru_A1943 0.78 +0.0 2,252,529 + Rru_A1943 0.78 -1.5 2,252,529 + Rru_A1943 0.78 +0.0 2,252,530 - Rru_A1943 0.78 -1.1 2,252,530 - Rru_A1943 0.78 -1.5 2,252,530 - Rru_A1943 0.78 +0.0 2,252,530 - Rru_A1943 0.78 -1.5 2,252,530 - Rru_A1943 0.78 +0.0 2,252,907 - -3.0 2,252,991 + -1.3 2,253,005 - -0.6 2,253,017 - -1.5 2,253,243 + Rru_A1944 0.42 -1.3 2,253,243 + Rru_A1944 0.42 +1.0 2,253,243 + Rru_A1944 0.42 -1.0 2,253,267 + Rru_A1944 0.46 -1.0 2,253,353 + Rru_A1944 0.60 -1.8 2,253,353 + Rru_A1944 0.60 -2.2 2,253,354 - Rru_A1944 0.60 -0.5 2,253,354 - Rru_A1944 0.60 -1.1 2,253,354 - Rru_A1944 0.60 -2.2 2,253,408 + Rru_A1944 0.69 -2.0 2,253,408 + Rru_A1944 0.69 -1.4 2,253,409 - Rru_A1944 0.69 -1.3 2,253,409 - Rru_A1944 0.69 -2.5 2,253,547 + -2.0 2,253,547 + -0.5 2,253,547 + +0.0 2,253,547 + +0.0 2,253,547 + +0.0 2,253,547 + -1.3 2,253,548 - -1.5 2,253,548 - +0.0 2,253,548 - +0.0 2,253,548 - -0.4 2,253,548 - +0.0 2,253,548 - -1.4 2,253,548 - -1.4 2,253,548 - -2.0 2,253,548 - -0.8 2,253,549 + +0.0 2,253,550 - +0.4 2,253,570 + -2.4 2,253,645 - -0.4 2,253,645 - -2.0 2,253,645 - -3.9 2,253,735 - +0.0 2,253,750 + -0.5 2,253,774 + +0.0 2,253,775 - +0.0 2,253,775 - -0.5 2,253,775 - -2.3 2,253,775 - -1.5 2,253,775 - +0.0
Or see this region's nucleotide sequence