Experiment: Succinic Acid; Light intensity 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0200 and Rru_A0201 are separated by 179 nucleotides Rru_A0201 and Rru_A0202 overlap by 4 nucleotides Rru_A0202 and Rru_A0203 are separated by 239 nucleotides Rru_A0203 and Rru_A0204 are separated by 249 nucleotides
Rru_A0200: Rru_A0200 - Restriction endonuclease (NCBI), at 248,466 to 249,677
_A0200
Rru_A0201: Rru_A0201 - Endonuclease/exonuclease/phosphatase (NCBI), at 249,857 to 250,651
_A0201
Rru_A0202: Rru_A0202 - Phospholipase D/Transphosphatidylase (NCBI), at 250,648 to 253,059
_A0202
Rru_A0203: Rru_A0203 - hypothetical protein (NCBI), at 253,299 to 253,607
_A0203
Rru_A0204: Rru_A0204 - BolA-like protein (NCBI), at 253,857 to 254,201
_A0204
Position (kb) 250
251
252
253
254 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 249.717 kb on - strand at 249.773 kb on - strand at 250.292 kb on + strand, within Rru_A0201 at 250.292 kb on + strand, within Rru_A0201 at 250.293 kb on - strand, within Rru_A0201 at 250.293 kb on - strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.472 kb on + strand, within Rru_A0201 at 250.473 kb on - strand, within Rru_A0201 at 250.473 kb on - strand, within Rru_A0201 at 250.925 kb on + strand, within Rru_A0202 at 250.926 kb on - strand, within Rru_A0202 at 250.942 kb on - strand, within Rru_A0202 at 251.751 kb on + strand, within Rru_A0202 at 251.852 kb on + strand, within Rru_A0202 at 251.966 kb on + strand, within Rru_A0202 at 251.967 kb on - strand, within Rru_A0202 at 252.152 kb on + strand, within Rru_A0202 at 252.152 kb on + strand, within Rru_A0202 at 252.153 kb on - strand, within Rru_A0202 at 252.153 kb on - strand, within Rru_A0202 at 252.153 kb on - strand, within Rru_A0202 at 252.242 kb on + strand, within Rru_A0202 at 252.243 kb on - strand, within Rru_A0202 at 252.729 kb on + strand, within Rru_A0202 at 252.729 kb on + strand, within Rru_A0202 at 252.729 kb on + strand, within Rru_A0202 at 252.729 kb on + strand, within Rru_A0202 at 252.730 kb on - strand, within Rru_A0202 at 252.730 kb on - strand, within Rru_A0202 at 252.920 kb on + strand at 252.920 kb on + strand at 252.921 kb on - strand at 252.921 kb on - strand at 252.921 kb on - strand at 253.118 kb on + strand at 253.118 kb on + strand at 253.518 kb on + strand, within Rru_A0203 at 253.598 kb on + strand at 253.598 kb on + strand at 253.669 kb on - strand at 253.704 kb on - strand at 253.726 kb on + strand at 253.726 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 150 uM remove 249,717 - -2.1 249,773 - +0.2 250,292 + Rru_A0201 0.55 -1.0 250,292 + Rru_A0201 0.55 -2.1 250,293 - Rru_A0201 0.55 -1.1 250,293 - Rru_A0201 0.55 +0.2 250,472 + Rru_A0201 0.77 +0.2 250,472 + Rru_A0201 0.77 +0.2 250,472 + Rru_A0201 0.77 -0.9 250,473 - Rru_A0201 0.77 -1.0 250,473 - Rru_A0201 0.77 -1.4 250,925 + Rru_A0202 0.11 -2.1 250,926 - Rru_A0202 0.12 -3.5 250,942 - Rru_A0202 0.12 -1.7 251,751 + Rru_A0202 0.46 -1.7 251,852 + Rru_A0202 0.50 +0.2 251,966 + Rru_A0202 0.55 -0.8 251,967 - Rru_A0202 0.55 +0.2 252,152 + Rru_A0202 0.62 -1.7 252,152 + Rru_A0202 0.62 -2.0 252,153 - Rru_A0202 0.62 -0.7 252,153 - Rru_A0202 0.62 +0.2 252,153 - Rru_A0202 0.62 -0.8 252,242 + Rru_A0202 0.66 +0.2 252,243 - Rru_A0202 0.66 +0.2 252,729 + Rru_A0202 0.86 -1.9 252,729 + Rru_A0202 0.86 +0.2 252,729 + Rru_A0202 0.86 -0.9 252,729 + Rru_A0202 0.86 +2.2 252,730 - Rru_A0202 0.86 -1.3 252,730 - Rru_A0202 0.86 -2.5 252,920 + -1.5 252,920 + +1.8 252,921 - -1.6 252,921 - -1.4 252,921 - -0.2 253,118 + -0.4 253,118 + +0.2 253,518 + Rru_A0203 0.71 -2.8 253,598 + -2.1 253,598 + +0.2 253,669 - -1.4 253,704 - -2.2 253,726 + +0.2 253,726 + -2.0
Or see this region's nucleotide sequence