Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24550

Experiment: Thymine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24535 and BWI76_RS24540 are separated by 8 nucleotidesBWI76_RS24540 and BWI76_RS24545 are separated by 13 nucleotidesBWI76_RS24545 and BWI76_RS24550 are separated by 233 nucleotidesBWI76_RS24550 and BWI76_RS24555 are separated by 273 nucleotides BWI76_RS24535: BWI76_RS24535 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 5,114,929 to 5,115,942 _RS24535 BWI76_RS24540: BWI76_RS24540 - hypothetical protein, at 5,115,951 to 5,116,166 _RS24540 BWI76_RS24545: BWI76_RS24545 - 30S ribosomal protein S21, at 5,116,180 to 5,116,395 _RS24545 BWI76_RS24550: BWI76_RS24550 - DNA primase, at 5,116,629 to 5,118,374 _RS24550 BWI76_RS24555: BWI76_RS24555 - RNA polymerase sigma factor RpoD, at 5,118,648 to 5,120,492 _RS24555 Position (kb) 5116 5117 5118 5119Strain fitness (log2 ratio) -3 -2 -1 0 1at 5115.956 kb on - strandat 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5116.003 kb on - strand, within BWI76_RS24540at 5116.012 kb on + strand, within BWI76_RS24540at 5116.017 kb on + strand, within BWI76_RS24540at 5116.526 kb on + strandat 5116.531 kb on + strandat 5118.338 kb on + strandat 5118.453 kb on + strandat 5118.453 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymine (N)
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5,115,956 - +0.6
5,115,982 - BWI76_RS24540 0.14 -0.6
5,115,982 - BWI76_RS24540 0.14 +0.5
5,115,982 - BWI76_RS24540 0.14 -0.6
5,115,982 - BWI76_RS24540 0.14 -1.0
5,115,982 - BWI76_RS24540 0.14 -0.1
5,115,982 - BWI76_RS24540 0.14 +0.8
5,115,982 - BWI76_RS24540 0.14 +0.8
5,116,003 - BWI76_RS24540 0.24 -0.8
5,116,012 + BWI76_RS24540 0.28 +1.0
5,116,017 + BWI76_RS24540 0.31 -0.6
5,116,526 + -0.8
5,116,531 + +0.3
5,118,338 + -2.9
5,118,453 + -0.4
5,118,453 + -3.1

Or see this region's nucleotide sequence