Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21490 and BWI76_RS21495 are separated by 11 nucleotides BWI76_RS21495 and BWI76_RS21500 are separated by 5 nucleotides BWI76_RS21500 and BWI76_RS21505 are separated by 54 nucleotides BWI76_RS21505 and BWI76_RS21510 are separated by 120 nucleotides BWI76_RS21510 and BWI76_RS21515 are separated by 39 nucleotides
BWI76_RS21490: BWI76_RS21490 - GGDEF domain-containing protein, at 4,501,737 to 4,502,960
_RS21490
BWI76_RS21495: BWI76_RS21495 - outer membrane lipoprotein, at 4,502,972 to 4,503,454
_RS21495
BWI76_RS21500: BWI76_RS21500 - membrane protein, at 4,503,460 to 4,504,830
_RS21500
BWI76_RS21505: BWI76_RS21505 - hypothetical protein, at 4,504,885 to 4,505,322
_RS21505
BWI76_RS21510: BWI76_RS21510 - 50S ribosomal protein L19, at 4,505,443 to 4,505,790
_RS21510
BWI76_RS21515: BWI76_RS21515 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 4,505,830 to 4,506,597
_RS21515
Position (kb) 4503
4504
4505 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4502.466 kb on + strand, within BWI76_RS21490 at 4502.490 kb on + strand, within BWI76_RS21490 at 4502.504 kb on + strand, within BWI76_RS21490 at 4502.512 kb on - strand, within BWI76_RS21490 at 4502.543 kb on + strand, within BWI76_RS21490 at 4502.567 kb on - strand, within BWI76_RS21490 at 4502.569 kb on + strand, within BWI76_RS21490 at 4502.599 kb on + strand, within BWI76_RS21490 at 4502.600 kb on + strand, within BWI76_RS21490 at 4502.608 kb on - strand, within BWI76_RS21490 at 4502.701 kb on - strand, within BWI76_RS21490 at 4502.758 kb on - strand, within BWI76_RS21490 at 4502.763 kb on + strand, within BWI76_RS21490 at 4502.856 kb on - strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.859 kb on + strand at 4502.867 kb on - strand at 4502.867 kb on - strand at 4502.867 kb on - strand at 4502.871 kb on + strand at 4502.879 kb on - strand at 4502.879 kb on - strand at 4502.884 kb on + strand at 4503.004 kb on - strand at 4503.089 kb on + strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.092 kb on - strand, within BWI76_RS21495 at 4503.097 kb on - strand at 4503.195 kb on + strand, within BWI76_RS21495 at 4503.203 kb on - strand, within BWI76_RS21495 at 4503.208 kb on - strand at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.224 kb on - strand, within BWI76_RS21495 at 4503.226 kb on + strand, within BWI76_RS21495 at 4503.240 kb on - strand, within BWI76_RS21495 at 4503.613 kb on - strand, within BWI76_RS21500 at 4503.647 kb on + strand, within BWI76_RS21500 at 4503.891 kb on + strand, within BWI76_RS21500 at 4504.376 kb on + strand, within BWI76_RS21500 at 4504.688 kb on - strand, within BWI76_RS21500 at 4504.802 kb on - strand at 4504.808 kb on - strand at 4504.940 kb on - strand, within BWI76_RS21505
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 4,502,466 + BWI76_RS21490 0.60 +1.6 4,502,490 + BWI76_RS21490 0.62 +0.1 4,502,504 + BWI76_RS21490 0.63 -1.0 4,502,512 - BWI76_RS21490 0.63 +0.4 4,502,543 + BWI76_RS21490 0.66 -0.4 4,502,567 - BWI76_RS21490 0.68 +0.6 4,502,569 + BWI76_RS21490 0.68 +2.6 4,502,599 + BWI76_RS21490 0.70 +1.7 4,502,600 + BWI76_RS21490 0.71 +0.3 4,502,608 - BWI76_RS21490 0.71 +0.7 4,502,701 - BWI76_RS21490 0.79 -1.2 4,502,758 - BWI76_RS21490 0.83 -2.3 4,502,763 + BWI76_RS21490 0.84 +0.0 4,502,856 - -0.2 4,502,859 + -0.6 4,502,859 + +0.0 4,502,859 + -0.4 4,502,859 + +0.4 4,502,867 - -0.0 4,502,867 - -0.5 4,502,867 - +0.1 4,502,871 + +1.4 4,502,879 - +0.3 4,502,879 - +1.3 4,502,884 + +0.5 4,503,004 - +1.5 4,503,089 + BWI76_RS21495 0.24 -4.0 4,503,092 - BWI76_RS21495 0.25 +2.5 4,503,092 - BWI76_RS21495 0.25 -1.4 4,503,092 - BWI76_RS21495 0.25 -1.9 4,503,092 - BWI76_RS21495 0.25 +1.1 4,503,097 - +0.8 4,503,195 + BWI76_RS21495 0.46 -0.7 4,503,203 - BWI76_RS21495 0.48 +0.2 4,503,208 - -1.2 4,503,224 - BWI76_RS21495 0.52 +0.2 4,503,224 - BWI76_RS21495 0.52 +0.4 4,503,224 - BWI76_RS21495 0.52 -3.5 4,503,226 + BWI76_RS21495 0.53 -0.2 4,503,240 - BWI76_RS21495 0.55 -0.8 4,503,613 - BWI76_RS21500 0.11 -2.2 4,503,647 + BWI76_RS21500 0.14 -1.7 4,503,891 + BWI76_RS21500 0.31 -3.9 4,504,376 + BWI76_RS21500 0.67 -0.7 4,504,688 - BWI76_RS21500 0.90 -2.1 4,504,802 - +2.2 4,504,808 - -0.2 4,504,940 - BWI76_RS21505 0.13 -0.4
Or see this region's nucleotide sequence