Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18145 and BWI76_RS18150 are separated by 8 nucleotides BWI76_RS18150 and BWI76_RS18155 are separated by 81 nucleotides BWI76_RS18155 and BWI76_RS18160 are separated by 37 nucleotides BWI76_RS18160 and BWI76_RS18165 are separated by 28 nucleotides
BWI76_RS18145: BWI76_RS18145 - Holliday junction branch migration DNA helicase RuvB, at 3,771,410 to 3,772,420
_RS18145
BWI76_RS18150: BWI76_RS18150 - Holliday junction branch migration protein RuvA, at 3,772,429 to 3,773,040
_RS18150
BWI76_RS18155: BWI76_RS18155 - crossover junction endodeoxyribonuclease RuvC, at 3,773,122 to 3,773,643
_RS18155
BWI76_RS18160: BWI76_RS18160 - YebC/PmpR family DNA-binding transcriptional regulator, at 3,773,681 to 3,774,421
_RS18160
BWI76_RS18165: BWI76_RS18165 - NUDIX pyrophosphatase, at 3,774,450 to 3,774,893
_RS18165
Position (kb) 3773
3774 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3774.045 kb on + strand, within BWI76_RS18160 at 3774.158 kb on - strand, within BWI76_RS18160 at 3774.396 kb on - strand at 3774.396 kb on - strand at 3774.501 kb on - strand, within BWI76_RS18165
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 3,774,045 + BWI76_RS18160 0.49 -1.2 3,774,158 - BWI76_RS18160 0.64 +0.9 3,774,396 - -2.2 3,774,396 - -3.8 3,774,501 - BWI76_RS18165 0.11 +0.1
Or see this region's nucleotide sequence