Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14955

Experiment: Thymine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14945 and BWI76_RS14950 are separated by 4 nucleotidesBWI76_RS14950 and BWI76_RS14955 are separated by 3 nucleotidesBWI76_RS14955 and BWI76_RS14960 are separated by 217 nucleotides BWI76_RS14945: BWI76_RS14945 - PTS system, cellobiose-specific IIC component, at 3,131,958 to 3,133,277 _RS14945 BWI76_RS14950: BWI76_RS14950 - glycosylasparaginase, at 3,133,282 to 3,134,235 _RS14950 BWI76_RS14955: BWI76_RS14955 - peptidase M17 leucyl aminopeptidase domain-containing protein, at 3,134,239 to 3,135,594 _RS14955 BWI76_RS14960: BWI76_RS14960 - MFS transporter, at 3,135,812 to 3,137,011 _RS14960 Position (kb) 3134 3135 3136Strain fitness (log2 ratio) -3 -2 -1 0 1at 3133.370 kb on + strandat 3133.421 kb on - strandat 3133.637 kb on + strand, within BWI76_RS14950at 3133.851 kb on - strand, within BWI76_RS14950at 3133.851 kb on - strand, within BWI76_RS14950at 3134.204 kb on - strandat 3134.232 kb on - strandat 3134.529 kb on - strand, within BWI76_RS14955at 3134.561 kb on + strand, within BWI76_RS14955at 3135.083 kb on + strand, within BWI76_RS14955at 3135.123 kb on + strand, within BWI76_RS14955at 3135.314 kb on + strand, within BWI76_RS14955at 3135.319 kb on + strand, within BWI76_RS14955at 3135.346 kb on + strand, within BWI76_RS14955at 3135.393 kb on + strand, within BWI76_RS14955at 3135.632 kb on - strandat 3135.733 kb on - strandat 3136.243 kb on + strand, within BWI76_RS14960at 3136.251 kb on - strand, within BWI76_RS14960at 3136.251 kb on - strand, within BWI76_RS14960at 3136.289 kb on - strand, within BWI76_RS14960at 3136.381 kb on - strand, within BWI76_RS14960at 3136.427 kb on + strand, within BWI76_RS14960at 3136.434 kb on + strand, within BWI76_RS14960

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymine (N)
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3,133,370 + +0.7
3,133,421 - +1.1
3,133,637 + BWI76_RS14950 0.37 +0.6
3,133,851 - BWI76_RS14950 0.60 -2.2
3,133,851 - BWI76_RS14950 0.60 +0.0
3,134,204 - +0.8
3,134,232 - -2.8
3,134,529 - BWI76_RS14955 0.21 -2.9
3,134,561 + BWI76_RS14955 0.24 -0.4
3,135,083 + BWI76_RS14955 0.62 -0.3
3,135,123 + BWI76_RS14955 0.65 +0.5
3,135,314 + BWI76_RS14955 0.79 +0.6
3,135,319 + BWI76_RS14955 0.80 -1.4
3,135,346 + BWI76_RS14955 0.82 -3.3
3,135,393 + BWI76_RS14955 0.85 -0.6
3,135,632 - +0.8
3,135,733 - -0.1
3,136,243 + BWI76_RS14960 0.36 -0.2
3,136,251 - BWI76_RS14960 0.37 +0.4
3,136,251 - BWI76_RS14960 0.37 -1.3
3,136,289 - BWI76_RS14960 0.40 +0.2
3,136,381 - BWI76_RS14960 0.47 -0.8
3,136,427 + BWI76_RS14960 0.51 -0.9
3,136,434 + BWI76_RS14960 0.52 -0.8

Or see this region's nucleotide sequence