Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11765 and BWI76_RS11770 are separated by 134 nucleotides BWI76_RS11770 and BWI76_RS11775 are separated by 91 nucleotides BWI76_RS11775 and BWI76_RS11780 are separated by 148 nucleotides BWI76_RS11780 and BWI76_RS11785 are separated by 130 nucleotides
BWI76_RS11765: BWI76_RS11765 - bifunctional transporter/kinase, at 2,475,327 to 2,476,718
_RS11765
BWI76_RS11770: BWI76_RS11770 - hypothetical protein, at 2,476,853 to 2,477,443
_RS11770
BWI76_RS11775: BWI76_RS11775 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,477,535 to 2,478,296
_RS11775
BWI76_RS11780: BWI76_RS11780 - 2-deoxyglucose-6-phosphatase, at 2,478,445 to 2,479,113
_RS11780
BWI76_RS11785: BWI76_RS11785 - hypothetical protein, at 2,479,244 to 2,479,780
_RS11785
Position (kb)
2477
2478
2479 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2476.638 kb on + strand at 2476.643 kb on - strand at 2476.937 kb on + strand, within BWI76_RS11770 at 2476.976 kb on - strand, within BWI76_RS11770 at 2477.275 kb on - strand, within BWI76_RS11770 at 2477.451 kb on + strand at 2477.575 kb on - strand at 2477.628 kb on - strand, within BWI76_RS11775 at 2477.829 kb on - strand, within BWI76_RS11775 at 2478.275 kb on + strand at 2478.283 kb on - strand at 2478.340 kb on + strand at 2478.613 kb on + strand, within BWI76_RS11780 at 2478.926 kb on + strand, within BWI76_RS11780 at 2479.249 kb on - strand at 2479.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 2,476,638 + -0.4 2,476,643 - -1.8 2,476,937 + BWI76_RS11770 0.14 -0.7 2,476,976 - BWI76_RS11770 0.21 -2.3 2,477,275 - BWI76_RS11770 0.71 -3.2 2,477,451 + +0.2 2,477,575 - +0.3 2,477,628 - BWI76_RS11775 0.12 +1.2 2,477,829 - BWI76_RS11775 0.39 +0.7 2,478,275 + +0.0 2,478,283 - -1.8 2,478,340 + +0.6 2,478,613 + BWI76_RS11780 0.25 -1.3 2,478,926 + BWI76_RS11780 0.72 -0.4 2,479,249 - +0.2 2,479,249 - +0.1
Or see this region's nucleotide sequence