Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05530 and BWI76_RS05535 are separated by 2 nucleotides BWI76_RS05535 and BWI76_RS05540 are separated by 111 nucleotides BWI76_RS05540 and BWI76_RS05545 are separated by 258 nucleotides BWI76_RS05545 and BWI76_RS05550 are separated by 106 nucleotides
BWI76_RS05530: BWI76_RS05530 - DNA polymerase IV, at 1,158,943 to 1,159,998
_RS05530
BWI76_RS05535: BWI76_RS05535 - acyltransferase, at 1,160,001 to 1,160,492
_RS05535
BWI76_RS05540: BWI76_RS05540 - cytosol nonspecific dipeptidase, at 1,160,604 to 1,162,061
_RS05540
BWI76_RS05545: BWI76_RS05545 - xanthine phosphoribosyltransferase, at 1,162,320 to 1,162,778
_RS05545
BWI76_RS05550: BWI76_RS05550 - fermentation/respiration switch protein, at 1,162,885 to 1,164,129
_RS05550
Position (kb)
1160
1161
1162
1163 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1159.886 kb on - strand, within BWI76_RS05530 at 1159.944 kb on + strand at 1159.969 kb on - strand at 1159.984 kb on + strand at 1159.991 kb on + strand at 1160.492 kb on + strand at 1160.586 kb on + strand at 1160.594 kb on - strand at 1160.644 kb on + strand at 1160.649 kb on + strand at 1160.649 kb on + strand at 1160.652 kb on - strand at 1160.715 kb on + strand at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.810 kb on - strand, within BWI76_RS05540 at 1161.053 kb on + strand, within BWI76_RS05540 at 1161.387 kb on + strand, within BWI76_RS05540 at 1161.387 kb on + strand, within BWI76_RS05540 at 1161.387 kb on + strand, within BWI76_RS05540 at 1161.395 kb on - strand, within BWI76_RS05540 at 1161.395 kb on - strand, within BWI76_RS05540 at 1161.395 kb on - strand, within BWI76_RS05540 at 1161.535 kb on + strand, within BWI76_RS05540 at 1161.537 kb on - strand, within BWI76_RS05540 at 1161.826 kb on + strand, within BWI76_RS05540 at 1161.948 kb on + strand at 1161.995 kb on + strand at 1162.151 kb on + strand at 1162.185 kb on + strand at 1162.193 kb on - strand at 1162.256 kb on - strand at 1162.302 kb on - strand at 1162.304 kb on + strand at 1162.312 kb on - strand at 1162.312 kb on - strand at 1162.314 kb on - strand at 1162.728 kb on - strand, within BWI76_RS05545 at 1162.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 1,159,886 - BWI76_RS05530 0.89 +0.2 1,159,944 + +0.1 1,159,969 - +3.5 1,159,984 + -0.6 1,159,991 + -0.2 1,160,492 + -1.7 1,160,586 + +0.5 1,160,594 - -0.8 1,160,644 + -0.8 1,160,649 + -3.4 1,160,649 + +0.1 1,160,652 - +0.7 1,160,715 + -1.0 1,160,763 + BWI76_RS05540 0.11 -1.9 1,160,763 + BWI76_RS05540 0.11 +0.9 1,160,763 + BWI76_RS05540 0.11 -1.7 1,160,810 - BWI76_RS05540 0.14 -3.4 1,161,053 + BWI76_RS05540 0.31 +0.1 1,161,387 + BWI76_RS05540 0.54 +1.3 1,161,387 + BWI76_RS05540 0.54 -0.5 1,161,387 + BWI76_RS05540 0.54 +0.4 1,161,395 - BWI76_RS05540 0.54 -0.1 1,161,395 - BWI76_RS05540 0.54 -0.1 1,161,395 - BWI76_RS05540 0.54 -1.3 1,161,535 + BWI76_RS05540 0.64 -0.7 1,161,537 - BWI76_RS05540 0.64 -0.4 1,161,826 + BWI76_RS05540 0.84 -0.6 1,161,948 + +0.5 1,161,995 + -4.0 1,162,151 + -0.2 1,162,185 + -0.2 1,162,193 - +1.9 1,162,256 - +0.3 1,162,302 - -0.3 1,162,304 + +1.0 1,162,312 - +0.0 1,162,312 - +0.3 1,162,314 - -1.0 1,162,728 - BWI76_RS05545 0.89 -0.4 1,162,989 - -1.7
Or see this region's nucleotide sequence