Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotides BWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides BWI76_RS05460 and BWI76_RS05465 are separated by 245 nucleotides
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429
_RS05450
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435
_RS05460
BWI76_RS05465: BWI76_RS05465 - amidohydrolase, at 1,145,681 to 1,146,451
_RS05465
Position (kb) 1144
1145
1146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1143.622 kb on + strand, within BWI76_RS05445 at 1143.641 kb on + strand, within BWI76_RS05445 at 1144.210 kb on + strand, within BWI76_RS05450 at 1144.317 kb on - strand, within BWI76_RS05450 at 1144.492 kb on + strand at 1145.308 kb on + strand at 1145.520 kb on + strand at 1145.628 kb on - strand at 1145.997 kb on - strand, within BWI76_RS05465
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 1,143,622 + BWI76_RS05445 0.52 +1.6 1,143,641 + BWI76_RS05445 0.55 -1.7 1,144,210 + BWI76_RS05450 0.53 +1.8 1,144,317 - BWI76_RS05450 0.76 -3.4 1,144,492 + +0.5 1,145,308 + -0.2 1,145,520 + +0.5 1,145,628 - -1.8 1,145,997 - BWI76_RS05465 0.41 -0.5
Or see this region's nucleotide sequence