Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05290

Experiment: Thymine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05280 and BWI76_RS05285 overlap by 4 nucleotidesBWI76_RS05285 and BWI76_RS05290 are separated by 38 nucleotidesBWI76_RS05290 and BWI76_RS05295 are separated by 12 nucleotides BWI76_RS05280: BWI76_RS05280 - lipid-A-disaccharide synthase, at 1,111,149 to 1,112,297 _RS05280 BWI76_RS05285: BWI76_RS05285 - ribonuclease HII, at 1,112,294 to 1,112,890 _RS05285 BWI76_RS05290: BWI76_RS05290 - DNA polymerase III subunit alpha, at 1,112,929 to 1,116,411 _RS05290 BWI76_RS05295: BWI76_RS05295 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 1,116,424 to 1,117,383 _RS05295 Position (kb) 1112 1113 1114 1115 1116 1117Strain fitness (log2 ratio) -1 0 1at 1112.362 kb on + strand, within BWI76_RS05285at 1112.850 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymine (N)
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1,112,362 + BWI76_RS05285 0.11 -0.8
1,112,850 + +0.7

Or see this region's nucleotide sequence