Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04605

Experiment: Thymine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04595 and BWI76_RS04600 are separated by 128 nucleotidesBWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotidesBWI76_RS04605 and BWI76_RS04610 overlap by 17 nucleotides BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623 _RS04595 BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519 _RS04600 BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207 _RS04605 BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801 _RS04610 Position (kb) 958 959 960Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 957.710 kb on - strandat 957.711 kb on + strandat 957.882 kb on + strand, within BWI76_RS04600at 957.910 kb on + strand, within BWI76_RS04600at 958.400 kb on + strand, within BWI76_RS04600at 958.852 kb on + strand, within BWI76_RS04605at 959.409 kb on - strand, within BWI76_RS04610at 959.490 kb on + strand, within BWI76_RS04610at 959.622 kb on - strand, within BWI76_RS04610at 959.760 kb on + strand, within BWI76_RS04610at 959.799 kb on + strand, within BWI76_RS04610at 959.944 kb on + strand, within BWI76_RS04610at 959.944 kb on + strand, within BWI76_RS04610at 960.041 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymine (N)
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957,710 - -0.2
957,711 + +1.6
957,882 + BWI76_RS04600 0.17 -0.9
957,910 + BWI76_RS04600 0.21 -0.1
958,400 + BWI76_RS04600 0.84 +0.3
958,852 + BWI76_RS04605 0.49 +1.8
959,409 - BWI76_RS04610 0.14 -3.2
959,490 + BWI76_RS04610 0.19 +0.5
959,622 - BWI76_RS04610 0.27 +0.3
959,760 + BWI76_RS04610 0.35 -2.1
959,799 + BWI76_RS04610 0.38 +0.6
959,944 + BWI76_RS04610 0.47 -0.1
959,944 + BWI76_RS04610 0.47 +1.1
960,041 - +0.1

Or see this region's nucleotide sequence