Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04440 and BWI76_RS04445 overlap by 4 nucleotides BWI76_RS04445 and BWI76_RS04450 are separated by 10 nucleotides BWI76_RS04450 and BWI76_RS04455 are separated by 19 nucleotides
BWI76_RS04440: BWI76_RS04440 - citrate lyase ACP, at 917,395 to 917,688
_RS04440
BWI76_RS04445: BWI76_RS04445 - citrate (pro-3S)-lyase subunit beta, at 917,685 to 918,554
_RS04445
BWI76_RS04450: BWI76_RS04450 - citrate lyase subunit alpha, at 918,565 to 920,091
_RS04450
BWI76_RS04455: BWI76_RS04455 - triphosphoribosyl-dephospho-CoA synthase CitG, at 920,111 to 921,019
_RS04455
Position (kb) 918
919
920
921 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 917.615 kb on - strand, within BWI76_RS04440 at 917.653 kb on + strand, within BWI76_RS04440 at 917.759 kb on - strand at 917.935 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.031 kb on + strand, within BWI76_RS04445 at 918.046 kb on + strand, within BWI76_RS04445 at 918.379 kb on - strand, within BWI76_RS04445 at 918.454 kb on + strand, within BWI76_RS04445 at 918.527 kb on - strand at 918.741 kb on + strand, within BWI76_RS04450 at 918.749 kb on - strand, within BWI76_RS04450 at 918.914 kb on + strand, within BWI76_RS04450 at 918.914 kb on + strand, within BWI76_RS04450 at 918.938 kb on + strand, within BWI76_RS04450 at 919.454 kb on + strand, within BWI76_RS04450 at 919.509 kb on + strand, within BWI76_RS04450 at 919.529 kb on - strand, within BWI76_RS04450 at 919.639 kb on + strand, within BWI76_RS04450 at 920.000 kb on - strand at 920.081 kb on - strand at 920.159 kb on - strand at 920.251 kb on + strand, within BWI76_RS04455 at 920.281 kb on - strand, within BWI76_RS04455 at 920.283 kb on + strand, within BWI76_RS04455 at 920.350 kb on + strand, within BWI76_RS04455 at 920.372 kb on - strand, within BWI76_RS04455 at 920.479 kb on - strand, within BWI76_RS04455 at 920.497 kb on - strand, within BWI76_RS04455 at 920.689 kb on + strand, within BWI76_RS04455 at 920.728 kb on + strand, within BWI76_RS04455 at 920.873 kb on + strand, within BWI76_RS04455 at 920.952 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 917,615 - BWI76_RS04440 0.75 -0.4 917,653 + BWI76_RS04440 0.88 +1.5 917,759 - -0.6 917,935 + BWI76_RS04445 0.29 -0.3 918,031 + BWI76_RS04445 0.40 -2.2 918,031 + BWI76_RS04445 0.40 +0.1 918,046 + BWI76_RS04445 0.41 -0.4 918,379 - BWI76_RS04445 0.80 +0.1 918,454 + BWI76_RS04445 0.88 +0.2 918,527 - -2.7 918,741 + BWI76_RS04450 0.12 -1.4 918,749 - BWI76_RS04450 0.12 -0.2 918,914 + BWI76_RS04450 0.23 +1.9 918,914 + BWI76_RS04450 0.23 +0.0 918,938 + BWI76_RS04450 0.24 -3.1 919,454 + BWI76_RS04450 0.58 -1.5 919,509 + BWI76_RS04450 0.62 +0.2 919,529 - BWI76_RS04450 0.63 -0.8 919,639 + BWI76_RS04450 0.70 -1.2 920,000 - -0.1 920,081 - +0.4 920,159 - -3.1 920,251 + BWI76_RS04455 0.15 -0.4 920,281 - BWI76_RS04455 0.19 -0.0 920,283 + BWI76_RS04455 0.19 -0.0 920,350 + BWI76_RS04455 0.26 -1.9 920,372 - BWI76_RS04455 0.29 -2.5 920,479 - BWI76_RS04455 0.40 -0.4 920,497 - BWI76_RS04455 0.42 +0.5 920,689 + BWI76_RS04455 0.64 -3.0 920,728 + BWI76_RS04455 0.68 -2.0 920,873 + BWI76_RS04455 0.84 -0.4 920,952 + -1.5
Or see this region's nucleotide sequence