Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotides BWI76_RS00890 and BWI76_RS00895 overlap by 56 nucleotides BWI76_RS00895 and BWI76_RS00900 overlap by 20 nucleotides
BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084
_RS00885
BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317
_RS00890
BWI76_RS00895: BWI76_RS00895 - glutamate racemase, at 185,262 to 186,116
_RS00895
BWI76_RS00900: BWI76_RS00900 - hypothetical protein, at 186,097 to 186,364
_RS00900
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 182.575 kb on - strand, within BWI76_RS00885 at 182.637 kb on + strand, within BWI76_RS00885 at 182.713 kb on + strand, within BWI76_RS00885 at 182.772 kb on + strand, within BWI76_RS00885 at 182.812 kb on - strand, within BWI76_RS00885 at 183.052 kb on + strand at 183.062 kb on + strand at 183.078 kb on - strand at 183.079 kb on + strand at 183.121 kb on - strand at 183.129 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.145 kb on + strand at 183.231 kb on + strand at 183.236 kb on + strand at 183.290 kb on - strand at 183.328 kb on + strand at 183.354 kb on - strand at 183.360 kb on - strand at 183.413 kb on - strand at 183.419 kb on - strand at 183.428 kb on + strand at 183.527 kb on - strand at 183.535 kb on + strand at 183.536 kb on - strand at 183.567 kb on + strand at 183.613 kb on - strand at 183.753 kb on - strand, within BWI76_RS00890 at 183.758 kb on + strand, within BWI76_RS00890 at 183.786 kb on + strand, within BWI76_RS00890 at 183.830 kb on + strand, within BWI76_RS00890 at 183.872 kb on + strand, within BWI76_RS00890 at 183.880 kb on - strand, within BWI76_RS00890 at 183.894 kb on - strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.945 kb on - strand, within BWI76_RS00890 at 183.951 kb on - strand, within BWI76_RS00890 at 184.005 kb on - strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.066 kb on + strand, within BWI76_RS00890 at 184.121 kb on - strand, within BWI76_RS00890 at 184.170 kb on - strand, within BWI76_RS00890 at 184.177 kb on - strand, within BWI76_RS00890 at 184.189 kb on - strand, within BWI76_RS00890 at 184.282 kb on + strand, within BWI76_RS00890 at 184.290 kb on + strand, within BWI76_RS00890 at 184.297 kb on + strand, within BWI76_RS00890 at 184.334 kb on - strand, within BWI76_RS00890 at 184.390 kb on + strand, within BWI76_RS00890 at 184.445 kb on + strand, within BWI76_RS00890 at 184.448 kb on - strand, within BWI76_RS00890 at 184.448 kb on - strand, within BWI76_RS00890 at 184.625 kb on + strand, within BWI76_RS00890 at 184.680 kb on + strand, within BWI76_RS00890 at 184.708 kb on - strand, within BWI76_RS00890 at 184.779 kb on - strand, within BWI76_RS00890 at 184.855 kb on + strand, within BWI76_RS00890 at 184.855 kb on + strand, within BWI76_RS00890 at 184.860 kb on + strand, within BWI76_RS00890 at 184.938 kb on - strand, within BWI76_RS00890 at 184.990 kb on + strand, within BWI76_RS00890 at 185.061 kb on - strand, within BWI76_RS00890 at 185.095 kb on + strand, within BWI76_RS00890 at 185.156 kb on - strand at 185.169 kb on + strand at 185.258 kb on + strand at 186.174 kb on + strand, within BWI76_RS00900
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 182,575 - BWI76_RS00885 0.54 +0.8 182,637 + BWI76_RS00885 0.59 -0.6 182,713 + BWI76_RS00885 0.66 -1.7 182,772 + BWI76_RS00885 0.72 +1.2 182,812 - BWI76_RS00885 0.75 -1.6 183,052 + -0.9 183,062 + -2.3 183,078 - -1.7 183,079 + -0.2 183,121 - -0.6 183,129 + -1.0 183,140 + +0.0 183,140 + +0.4 183,140 + -0.6 183,145 + +1.2 183,231 + +0.9 183,236 + +0.3 183,290 - -1.6 183,328 + +1.5 183,354 - +4.5 183,360 - +1.4 183,413 - +0.7 183,419 - +1.4 183,428 + -1.1 183,527 - -0.4 183,535 + +0.7 183,536 - +0.7 183,567 + +1.1 183,613 - -1.9 183,753 - BWI76_RS00890 0.16 +0.7 183,758 + BWI76_RS00890 0.17 +2.7 183,786 + BWI76_RS00890 0.18 +2.6 183,830 + BWI76_RS00890 0.20 +2.0 183,872 + BWI76_RS00890 0.23 +1.6 183,880 - BWI76_RS00890 0.23 +0.7 183,894 - BWI76_RS00890 0.24 +0.0 183,942 + BWI76_RS00890 0.26 +0.2 183,942 + BWI76_RS00890 0.26 +0.6 183,942 + BWI76_RS00890 0.26 +0.0 183,945 - BWI76_RS00890 0.27 +1.5 183,951 - BWI76_RS00890 0.27 +1.5 184,005 - BWI76_RS00890 0.30 -0.2 184,017 + BWI76_RS00890 0.30 -0.0 184,017 + BWI76_RS00890 0.30 +0.6 184,066 + BWI76_RS00890 0.33 +2.3 184,121 - BWI76_RS00890 0.36 -0.6 184,170 - BWI76_RS00890 0.39 +0.8 184,177 - BWI76_RS00890 0.39 +2.6 184,189 - BWI76_RS00890 0.40 +1.0 184,282 + BWI76_RS00890 0.45 +0.3 184,290 + BWI76_RS00890 0.45 +0.1 184,297 + BWI76_RS00890 0.45 -2.3 184,334 - BWI76_RS00890 0.47 +0.2 184,390 + BWI76_RS00890 0.50 +0.8 184,445 + BWI76_RS00890 0.53 +0.2 184,448 - BWI76_RS00890 0.53 -1.7 184,448 - BWI76_RS00890 0.53 +1.6 184,625 + BWI76_RS00890 0.63 -1.2 184,680 + BWI76_RS00890 0.66 +1.3 184,708 - BWI76_RS00890 0.67 +0.7 184,779 - BWI76_RS00890 0.71 -0.5 184,855 + BWI76_RS00890 0.75 -0.3 184,855 + BWI76_RS00890 0.75 +0.6 184,860 + BWI76_RS00890 0.75 +0.2 184,938 - BWI76_RS00890 0.80 -1.6 184,990 + BWI76_RS00890 0.82 -2.8 185,061 - BWI76_RS00890 0.86 +1.2 185,095 + BWI76_RS00890 0.88 +0.8 185,156 - -0.6 185,169 + -2.9 185,258 + +0.4 186,174 + BWI76_RS00900 0.29 -1.3
Or see this region's nucleotide sequence