Experiment: Succinic Acid; Light intensity 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2474 and Rru_A2475 are separated by 344 nucleotides Rru_A2475 and Rru_A2476 overlap by 8 nucleotides
Rru_A2474: Rru_A2474 - extracellular solute-binding protein, family 5 (NCBI), at 2,873,323 to 2,874,900
_A2474
Rru_A2475: Rru_A2475 - Glycine betaine/L-proline transport ATP-binding subunit (NCBI), at 2,875,245 to 2,876,444
_A2475
Rru_A2476: Rru_A2476 - Binding-protein-dependent transport systems inner membrane component (NCBI), at 2,876,437 to 2,877,510
_A2476
Position (kb)
2875
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2874.475 kb on - strand, within Rru_A2474 at 2874.475 kb on - strand, within Rru_A2474 at 2874.522 kb on - strand, within Rru_A2474 at 2874.522 kb on - strand, within Rru_A2474 at 2874.574 kb on - strand, within Rru_A2474 at 2874.662 kb on - strand, within Rru_A2474 at 2874.775 kb on + strand at 2874.775 kb on + strand at 2874.776 kb on - strand at 2874.776 kb on - strand at 2874.963 kb on + strand at 2874.963 kb on + strand at 2874.964 kb on - strand at 2875.276 kb on - strand at 2875.276 kb on - strand at 2876.022 kb on + strand, within Rru_A2475 at 2876.022 kb on + strand, within Rru_A2475 at 2876.022 kb on + strand, within Rru_A2475 at 2876.023 kb on - strand, within Rru_A2475 at 2876.023 kb on - strand, within Rru_A2475 at 2876.023 kb on - strand, within Rru_A2475 at 2876.023 kb on - strand, within Rru_A2475 at 2876.166 kb on + strand, within Rru_A2475 at 2876.167 kb on - strand, within Rru_A2475 at 2876.173 kb on - strand, within Rru_A2475 at 2876.173 kb on - strand, within Rru_A2475 at 2876.376 kb on + strand at 2876.972 kb on - strand, within Rru_A2476 at 2877.406 kb on + strand at 2877.407 kb on - strand at 2877.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 150 uM remove 2,874,475 - Rru_A2474 0.73 +0.1 2,874,475 - Rru_A2474 0.73 -3.7 2,874,522 - Rru_A2474 0.76 -1.2 2,874,522 - Rru_A2474 0.76 -0.7 2,874,574 - Rru_A2474 0.79 -1.2 2,874,662 - Rru_A2474 0.85 +0.1 2,874,775 + -0.4 2,874,775 + +0.1 2,874,776 - -0.3 2,874,776 - +0.1 2,874,963 + -0.0 2,874,963 + -0.7 2,874,964 - +0.1 2,875,276 - -1.1 2,875,276 - -1.3 2,876,022 + Rru_A2475 0.65 +0.1 2,876,022 + Rru_A2475 0.65 -0.7 2,876,022 + Rru_A2475 0.65 +0.1 2,876,023 - Rru_A2475 0.65 -0.6 2,876,023 - Rru_A2475 0.65 -0.7 2,876,023 - Rru_A2475 0.65 -0.7 2,876,023 - Rru_A2475 0.65 -0.5 2,876,166 + Rru_A2475 0.77 -0.8 2,876,167 - Rru_A2475 0.77 +0.1 2,876,173 - Rru_A2475 0.77 -0.8 2,876,173 - Rru_A2475 0.77 -1.1 2,876,376 + -0.7 2,876,972 - Rru_A2476 0.50 +0.1 2,877,406 + -1.1 2,877,407 - -1.1 2,877,407 - +0.1
Or see this region's nucleotide sequence