Experiment: Succinic Acid; Light intensity 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0306 and Rru_A0307 overlap by 4 nucleotides Rru_A0307 and Rru_A0308 are separated by 0 nucleotides Rru_A0308 and Rru_A0309 are separated by 492 nucleotides
Rru_A0306: Rru_A0306 - Hydrogenase formation HypD protein (NCBI), at 373,037 to 374,197
_A0306
Rru_A0307: Rru_A0307 - Hydrogenase expression/formation protein (HUPF/HYPC) (NCBI), at 374,194 to 374,424
_A0307
Rru_A0308: Rru_A0308 - Hydrogenase maturation protein HypF (NCBI), at 374,425 to 376,731
_A0308
Rru_A0309: Rru_A0309 - 4Fe-4S ferredoxin, iron-sulfur binding (NCBI), at 377,224 to 377,862
_A0309
Position (kb) 374
375
376
377 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 373.917 kb on + strand, within Rru_A0306 at 373.917 kb on + strand, within Rru_A0306 at 373.917 kb on + strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 374.326 kb on + strand, within Rru_A0307 at 374.326 kb on + strand, within Rru_A0307 at 374.326 kb on + strand, within Rru_A0307 at 374.327 kb on - strand, within Rru_A0307 at 374.327 kb on - strand, within Rru_A0307 at 374.426 kb on + strand at 374.426 kb on + strand at 374.426 kb on + strand at 374.426 kb on + strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.456 kb on - strand at 374.468 kb on + strand at 374.469 kb on - strand at 374.556 kb on + strand at 374.557 kb on - strand at 374.829 kb on - strand, within Rru_A0308 at 374.924 kb on - strand, within Rru_A0308 at 375.186 kb on + strand, within Rru_A0308 at 375.397 kb on - strand, within Rru_A0308 at 375.919 kb on + strand, within Rru_A0308 at 375.919 kb on + strand, within Rru_A0308 at 375.919 kb on + strand, within Rru_A0308 at 375.919 kb on + strand, within Rru_A0308 at 375.920 kb on - strand, within Rru_A0308 at 376.101 kb on + strand, within Rru_A0308 at 376.188 kb on - strand, within Rru_A0308 at 376.708 kb on + strand at 376.821 kb on + strand at 376.821 kb on + strand at 376.822 kb on - strand at 376.822 kb on - strand at 376.863 kb on - strand at 377.084 kb on - strand at 377.202 kb on + strand at 377.203 kb on - strand at 377.203 kb on - strand at 377.644 kb on + strand, within Rru_A0309 at 377.644 kb on + strand, within Rru_A0309 at 377.645 kb on - strand, within Rru_A0309 at 377.645 kb on - strand, within Rru_A0309 at 377.645 kb on - strand, within Rru_A0309 at 377.647 kb on - strand, within Rru_A0309 at 377.647 kb on - strand, within Rru_A0309 at 377.693 kb on - strand, within Rru_A0309 at 377.693 kb on - strand, within Rru_A0309
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 150 uM remove 373,917 + Rru_A0306 0.76 +0.1 373,917 + Rru_A0306 0.76 -3.1 373,917 + Rru_A0306 0.76 +0.5 373,918 - Rru_A0306 0.76 -2.2 373,918 - Rru_A0306 0.76 -1.4 373,918 - Rru_A0306 0.76 +0.1 373,918 - Rru_A0306 0.76 -0.8 374,326 + Rru_A0307 0.57 -0.4 374,326 + Rru_A0307 0.57 -0.5 374,326 + Rru_A0307 0.57 -0.6 374,327 - Rru_A0307 0.58 -0.9 374,327 - Rru_A0307 0.58 -1.8 374,426 + +0.1 374,426 + +0.1 374,426 + -0.5 374,426 + -0.5 374,427 - +0.1 374,427 - -0.4 374,427 - +0.1 374,427 - +0.1 374,456 - -0.6 374,468 + +0.1 374,469 - +0.1 374,556 + -2.0 374,557 - +0.1 374,829 - Rru_A0308 0.18 -1.6 374,924 - Rru_A0308 0.22 -1.8 375,186 + Rru_A0308 0.33 -1.5 375,397 - Rru_A0308 0.42 -1.4 375,919 + Rru_A0308 0.65 +0.1 375,919 + Rru_A0308 0.65 +0.1 375,919 + Rru_A0308 0.65 +1.1 375,919 + Rru_A0308 0.65 -1.2 375,920 - Rru_A0308 0.65 +0.4 376,101 + Rru_A0308 0.73 +0.1 376,188 - Rru_A0308 0.76 +0.1 376,708 + -1.5 376,821 + +0.1 376,821 + -0.9 376,822 - +0.1 376,822 - +0.1 376,863 - -1.2 377,084 - +0.1 377,202 + +0.1 377,203 - +0.1 377,203 - -0.9 377,644 + Rru_A0309 0.66 +0.1 377,644 + Rru_A0309 0.66 +0.1 377,645 - Rru_A0309 0.66 +0.1 377,645 - Rru_A0309 0.66 +0.1 377,645 - Rru_A0309 0.66 -2.0 377,647 - Rru_A0309 0.66 -0.8 377,647 - Rru_A0309 0.66 +0.1 377,693 - Rru_A0309 0.73 +0.1 377,693 - Rru_A0309 0.73 -1.7
Or see this region's nucleotide sequence