Experiment: H2 N-free with 2g/L casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01330 and MMP_RS01335 are separated by 37 nucleotides MMP_RS01335 and MMP_RS01340 are separated by 57 nucleotides MMP_RS01340 and MMP_RS01345 are separated by 7 nucleotides MMP_RS01345 and MMP_RS01350 overlap by 1 nucleotides
MMP_RS01330: MMP_RS01330 - DHH family phosphoesterase, at 248,751 to 249,767
_RS01330
MMP_RS01335: MMP_RS01335 - prefoldin subunit beta, at 249,805 to 250,146
_RS01335
MMP_RS01340: MMP_RS01340 - KEOPS complex Pcc1-like subunit, at 250,204 to 250,455
_RS01340
MMP_RS01345: MMP_RS01345 - rRNA maturation protein, at 250,463 to 250,957
_RS01345
MMP_RS01350: MMP_RS01350 - DNA-directed RNA polymerase subunit P, at 250,957 to 251,097
_RS01350
Position (kb) 249
250
251 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 248.973 kb on - strand, within MMP_RS01330 at 248.987 kb on - strand, within MMP_RS01330 at 249.032 kb on - strand, within MMP_RS01330 at 249.033 kb on + strand, within MMP_RS01330 at 249.036 kb on - strand, within MMP_RS01330 at 249.036 kb on - strand, within MMP_RS01330 at 249.090 kb on + strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.094 kb on - strand, within MMP_RS01330 at 249.095 kb on + strand, within MMP_RS01330 at 249.095 kb on + strand, within MMP_RS01330 at 249.098 kb on - strand, within MMP_RS01330 at 249.126 kb on + strand, within MMP_RS01330 at 249.181 kb on - strand, within MMP_RS01330 at 249.201 kb on - strand, within MMP_RS01330 at 249.201 kb on - strand, within MMP_RS01330 at 249.202 kb on + strand, within MMP_RS01330 at 249.202 kb on + strand, within MMP_RS01330 at 249.203 kb on - strand, within MMP_RS01330 at 249.203 kb on - strand, within MMP_RS01330 at 249.225 kb on - strand, within MMP_RS01330 at 249.230 kb on - strand, within MMP_RS01330 at 249.231 kb on + strand, within MMP_RS01330 at 249.267 kb on + strand, within MMP_RS01330 at 249.267 kb on + strand, within MMP_RS01330 at 249.271 kb on - strand, within MMP_RS01330 at 249.437 kb on - strand, within MMP_RS01330 at 249.449 kb on - strand, within MMP_RS01330 at 249.450 kb on + strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.534 kb on - strand, within MMP_RS01330 at 249.555 kb on - strand, within MMP_RS01330 at 249.588 kb on - strand, within MMP_RS01330 at 249.604 kb on + strand, within MMP_RS01330 at 249.714 kb on - strand at 249.790 kb on - strand at 249.806 kb on - strand at 249.854 kb on - strand, within MMP_RS01335 at 249.891 kb on - strand, within MMP_RS01335 at 250.181 kb on + strand at 250.192 kb on + strand at 250.202 kb on - strand at 250.206 kb on + strand at 250.226 kb on - strand at 250.226 kb on - strand at 250.227 kb on + strand at 250.260 kb on + strand at 250.265 kb on - strand at 250.458 kb on - strand at 250.603 kb on + strand at 250.606 kb on - strand at 250.811 kb on - strand at 250.811 kb on - strand at 250.812 kb on + strand at 250.818 kb on + strand at 250.977 kb on - strand at 250.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos remove 248,973 - MMP_RS01330 0.22 -1.0 248,987 - MMP_RS01330 0.23 -0.7 249,032 - MMP_RS01330 0.28 -0.2 249,033 + MMP_RS01330 0.28 -0.9 249,036 - MMP_RS01330 0.28 -1.0 249,036 - MMP_RS01330 0.28 -0.7 249,090 + MMP_RS01330 0.33 -0.9 249,094 - MMP_RS01330 0.34 -0.3 249,094 - MMP_RS01330 0.34 +0.4 249,094 - MMP_RS01330 0.34 -0.2 249,095 + MMP_RS01330 0.34 -1.8 249,095 + MMP_RS01330 0.34 +0.5 249,098 - MMP_RS01330 0.34 -0.5 249,126 + MMP_RS01330 0.37 -1.5 249,181 - MMP_RS01330 0.42 -0.4 249,201 - MMP_RS01330 0.44 -0.7 249,201 - MMP_RS01330 0.44 -0.3 249,202 + MMP_RS01330 0.44 -2.6 249,202 + MMP_RS01330 0.44 -1.3 249,203 - MMP_RS01330 0.44 +1.7 249,203 - MMP_RS01330 0.44 -0.4 249,225 - MMP_RS01330 0.47 -0.3 249,230 - MMP_RS01330 0.47 -1.1 249,231 + MMP_RS01330 0.47 -1.0 249,267 + MMP_RS01330 0.51 -0.8 249,267 + MMP_RS01330 0.51 -0.6 249,271 - MMP_RS01330 0.51 +0.1 249,437 - MMP_RS01330 0.67 -1.4 249,449 - MMP_RS01330 0.69 -2.1 249,450 + MMP_RS01330 0.69 +0.2 249,534 - MMP_RS01330 0.77 -0.3 249,534 - MMP_RS01330 0.77 -0.2 249,534 - MMP_RS01330 0.77 -0.2 249,555 - MMP_RS01330 0.79 -0.8 249,588 - MMP_RS01330 0.82 -0.5 249,604 + MMP_RS01330 0.84 -0.4 249,714 - -0.8 249,790 - -0.5 249,806 - -0.2 249,854 - MMP_RS01335 0.14 -0.8 249,891 - MMP_RS01335 0.25 -1.6 250,181 + -0.3 250,192 + -1.5 250,202 - -0.3 250,206 + -0.2 250,226 - -3.0 250,226 - -3.9 250,227 + -2.6 250,260 + -2.0 250,265 - -2.3 250,458 - -2.0 250,603 + -2.9 250,606 - -1.3 250,811 - -0.4 250,811 - -1.3 250,812 + +0.6 250,818 + -2.3 250,977 - -3.4 250,993 - -0.4
Or see this region's nucleotide sequence