Experiment: Cytidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09570 and BWI76_RS09575 are separated by 34 nucleotides BWI76_RS09575 and BWI76_RS09580 overlap by 93 nucleotides BWI76_RS09580 and BWI76_RS09585 are separated by 124 nucleotides
BWI76_RS09570: BWI76_RS09570 - hydroxylamine reductase, at 2,001,748 to 2,003,400
_RS09570
BWI76_RS09575: BWI76_RS09575 - hypothetical protein, at 2,003,435 to 2,003,638
_RS09575
BWI76_RS09580: BWI76_RS09580 - surface protein, at 2,003,546 to 2,004,445
_RS09580
BWI76_RS09585: BWI76_RS09585 - aquaporin, at 2,004,570 to 2,005,265
_RS09585
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2002.592 kb on + strand, within BWI76_RS09570 at 2002.643 kb on + strand, within BWI76_RS09570 at 2002.668 kb on + strand, within BWI76_RS09570 at 2002.859 kb on + strand, within BWI76_RS09570 at 2003.335 kb on - strand at 2003.350 kb on + strand at 2003.373 kb on + strand at 2003.799 kb on - strand, within BWI76_RS09580 at 2003.889 kb on - strand, within BWI76_RS09580 at 2003.908 kb on + strand, within BWI76_RS09580 at 2003.908 kb on + strand, within BWI76_RS09580 at 2003.916 kb on - strand, within BWI76_RS09580 at 2003.916 kb on - strand, within BWI76_RS09580 at 2003.926 kb on - strand, within BWI76_RS09580 at 2003.974 kb on + strand, within BWI76_RS09580 at 2003.995 kb on + strand, within BWI76_RS09580 at 2003.995 kb on + strand, within BWI76_RS09580 at 2004.003 kb on - strand, within BWI76_RS09580 at 2004.003 kb on - strand, within BWI76_RS09580 at 2004.108 kb on + strand, within BWI76_RS09580 at 2004.116 kb on - strand, within BWI76_RS09580 at 2004.116 kb on - strand, within BWI76_RS09580 at 2004.123 kb on - strand, within BWI76_RS09580 at 2004.198 kb on + strand, within BWI76_RS09580 at 2004.198 kb on + strand, within BWI76_RS09580 at 2004.203 kb on + strand, within BWI76_RS09580 at 2004.206 kb on - strand, within BWI76_RS09580 at 2004.206 kb on - strand, within BWI76_RS09580 at 2004.226 kb on + strand, within BWI76_RS09580 at 2004.231 kb on + strand, within BWI76_RS09580 at 2004.307 kb on - strand, within BWI76_RS09580 at 2004.372 kb on + strand at 2004.391 kb on + strand at 2004.401 kb on + strand at 2004.402 kb on + strand at 2004.416 kb on - strand at 2004.507 kb on + strand at 2004.507 kb on + strand at 2004.507 kb on + strand at 2004.507 kb on + strand at 2004.515 kb on - strand at 2004.515 kb on - strand at 2004.515 kb on - strand at 2004.590 kb on + strand at 2004.592 kb on + strand at 2004.598 kb on - strand at 2004.645 kb on - strand, within BWI76_RS09585 at 2004.746 kb on + strand, within BWI76_RS09585 at 2004.840 kb on + strand, within BWI76_RS09585 at 2004.840 kb on + strand, within BWI76_RS09585 at 2004.840 kb on + strand, within BWI76_RS09585 at 2004.848 kb on - strand, within BWI76_RS09585 at 2004.901 kb on + strand, within BWI76_RS09585 at 2004.901 kb on + strand, within BWI76_RS09585 at 2004.936 kb on + strand, within BWI76_RS09585 at 2004.942 kb on - strand, within BWI76_RS09585 at 2004.965 kb on - strand, within BWI76_RS09585 at 2004.965 kb on - strand, within BWI76_RS09585 at 2004.966 kb on + strand, within BWI76_RS09585 at 2004.974 kb on - strand, within BWI76_RS09585 at 2004.979 kb on - strand, within BWI76_RS09585 at 2005.059 kb on + strand, within BWI76_RS09585 at 2005.061 kb on - strand, within BWI76_RS09585 at 2005.066 kb on - strand, within BWI76_RS09585 at 2005.113 kb on + strand, within BWI76_RS09585 at 2005.113 kb on + strand, within BWI76_RS09585 at 2005.113 kb on + strand, within BWI76_RS09585 at 2005.113 kb on + strand, within BWI76_RS09585 at 2005.121 kb on - strand, within BWI76_RS09585 at 2005.143 kb on - strand, within BWI76_RS09585 at 2005.179 kb on + strand, within BWI76_RS09585 at 2005.183 kb on + strand, within BWI76_RS09585 at 2005.211 kb on - strand at 2005.212 kb on + strand at 2005.247 kb on - strand at 2005.247 kb on - strand at 2005.350 kb on - strand at 2005.360 kb on - strand at 2005.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (N) remove 2,002,592 + BWI76_RS09570 0.51 +0.4 2,002,643 + BWI76_RS09570 0.54 -1.2 2,002,668 + BWI76_RS09570 0.56 -0.3 2,002,859 + BWI76_RS09570 0.67 -0.3 2,003,335 - -0.7 2,003,350 + +0.6 2,003,373 + +0.9 2,003,799 - BWI76_RS09580 0.28 -1.1 2,003,889 - BWI76_RS09580 0.38 -0.5 2,003,908 + BWI76_RS09580 0.40 -1.7 2,003,908 + BWI76_RS09580 0.40 -1.2 2,003,916 - BWI76_RS09580 0.41 -2.2 2,003,916 - BWI76_RS09580 0.41 -1.6 2,003,926 - BWI76_RS09580 0.42 -2.1 2,003,974 + BWI76_RS09580 0.48 -2.3 2,003,995 + BWI76_RS09580 0.50 -1.4 2,003,995 + BWI76_RS09580 0.50 -2.8 2,004,003 - BWI76_RS09580 0.51 -1.7 2,004,003 - BWI76_RS09580 0.51 -1.2 2,004,108 + BWI76_RS09580 0.62 -1.1 2,004,116 - BWI76_RS09580 0.63 +0.1 2,004,116 - BWI76_RS09580 0.63 -1.6 2,004,123 - BWI76_RS09580 0.64 -2.7 2,004,198 + BWI76_RS09580 0.72 -2.6 2,004,198 + BWI76_RS09580 0.72 +0.3 2,004,203 + BWI76_RS09580 0.73 -1.7 2,004,206 - BWI76_RS09580 0.73 -1.8 2,004,206 - BWI76_RS09580 0.73 -1.3 2,004,226 + BWI76_RS09580 0.76 -2.1 2,004,231 + BWI76_RS09580 0.76 -1.9 2,004,307 - BWI76_RS09580 0.85 -3.3 2,004,372 + -1.5 2,004,391 + -1.5 2,004,401 + -1.2 2,004,402 + -1.5 2,004,416 - -2.0 2,004,507 + -0.6 2,004,507 + +0.2 2,004,507 + +0.4 2,004,507 + +0.7 2,004,515 - +1.1 2,004,515 - +0.1 2,004,515 - -0.2 2,004,590 + +0.2 2,004,592 + +0.6 2,004,598 - +0.3 2,004,645 - BWI76_RS09585 0.11 -0.7 2,004,746 + BWI76_RS09585 0.25 +0.3 2,004,840 + BWI76_RS09585 0.39 -0.1 2,004,840 + BWI76_RS09585 0.39 +0.2 2,004,840 + BWI76_RS09585 0.39 -0.0 2,004,848 - BWI76_RS09585 0.40 -0.0 2,004,901 + BWI76_RS09585 0.48 +1.2 2,004,901 + BWI76_RS09585 0.48 +0.3 2,004,936 + BWI76_RS09585 0.53 -0.9 2,004,942 - BWI76_RS09585 0.53 -0.2 2,004,965 - BWI76_RS09585 0.57 +1.0 2,004,965 - BWI76_RS09585 0.57 +0.7 2,004,966 + BWI76_RS09585 0.57 +0.8 2,004,974 - BWI76_RS09585 0.58 +0.0 2,004,979 - BWI76_RS09585 0.59 +0.1 2,005,059 + BWI76_RS09585 0.70 -1.2 2,005,061 - BWI76_RS09585 0.71 -0.1 2,005,066 - BWI76_RS09585 0.71 +0.3 2,005,113 + BWI76_RS09585 0.78 -0.8 2,005,113 + BWI76_RS09585 0.78 -0.3 2,005,113 + BWI76_RS09585 0.78 +0.5 2,005,113 + BWI76_RS09585 0.78 -2.1 2,005,121 - BWI76_RS09585 0.79 +0.8 2,005,143 - BWI76_RS09585 0.82 +0.9 2,005,179 + BWI76_RS09585 0.88 +0.3 2,005,183 + BWI76_RS09585 0.88 +1.2 2,005,211 - +0.0 2,005,212 + +1.6 2,005,247 - -0.9 2,005,247 - -0.1 2,005,350 - -0.6 2,005,360 - +0.5 2,005,371 + -0.0
Or see this region's nucleotide sequence