Experiment: 4C survival; 192 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25680 and AO353_25685 are separated by 9 nucleotides AO353_25685 and AO353_25690 are separated by 12 nucleotides AO353_25690 and AO353_25695 are separated by 16 nucleotides
AO353_25680: AO353_25680 - 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (from data) , at 5,662,861 to 5,663,988
_25680
AO353_25685: AO353_25685 - acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (from data) , at 5,663,998 to 5,665,191
_25685
AO353_25690: AO353_25690 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 5,665,204 to 5,665,965
_25690
AO353_25695: AO353_25695 - AMP-binding protein, at 5,665,982 to 5,667,646
_25695
Position (kb)
5663
5664
5665
5666 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5663.093 kb on + strand, within AO353_25680 at 5663.093 kb on + strand, within AO353_25680 at 5663.301 kb on - strand, within AO353_25680 at 5663.417 kb on + strand, within AO353_25680 at 5663.417 kb on + strand, within AO353_25680 at 5663.523 kb on + strand, within AO353_25680 at 5663.524 kb on - strand, within AO353_25680 at 5663.552 kb on + strand, within AO353_25680 at 5663.552 kb on + strand, within AO353_25680 at 5663.553 kb on - strand at 5663.553 kb on - strand, within AO353_25680 at 5663.790 kb on - strand, within AO353_25680 at 5663.790 kb on - strand, within AO353_25680 at 5663.799 kb on - strand, within AO353_25680 at 5663.966 kb on - strand at 5663.966 kb on - strand at 5663.983 kb on + strand at 5663.999 kb on + strand at 5663.999 kb on + strand at 5663.999 kb on + strand at 5663.999 kb on + strand at 5663.999 kb on + strand at 5664.000 kb on - strand at 5664.002 kb on + strand at 5664.003 kb on - strand at 5664.562 kb on + strand, within AO353_25685 at 5664.562 kb on + strand, within AO353_25685 at 5664.562 kb on + strand, within AO353_25685 at 5664.563 kb on - strand, within AO353_25685 at 5664.563 kb on - strand, within AO353_25685 at 5664.725 kb on + strand, within AO353_25685 at 5664.726 kb on - strand, within AO353_25685 at 5664.726 kb on - strand, within AO353_25685 at 5664.726 kb on - strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.812 kb on + strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5664.813 kb on - strand, within AO353_25685 at 5665.036 kb on + strand, within AO353_25685 at 5665.036 kb on + strand, within AO353_25685 at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.198 kb on + strand at 5665.199 kb on - strand at 5665.199 kb on - strand at 5665.205 kb on + strand at 5665.205 kb on + strand at 5665.206 kb on - strand at 5665.206 kb on - strand at 5665.275 kb on + strand at 5665.275 kb on + strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5665.276 kb on - strand at 5666.045 kb on - strand at 5666.045 kb on - strand at 5666.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 192 hrs remove 5,663,093 + AO353_25680 0.21 -0.2 5,663,093 + AO353_25680 0.21 +0.0 5,663,301 - AO353_25680 0.39 +0.6 5,663,417 + AO353_25680 0.49 -0.4 5,663,417 + AO353_25680 0.49 -0.4 5,663,523 + AO353_25680 0.59 +0.1 5,663,524 - AO353_25680 0.59 -0.4 5,663,552 + AO353_25680 0.61 +1.1 5,663,552 + AO353_25680 0.61 +0.2 5,663,553 - +0.1 5,663,553 - AO353_25680 0.61 +0.7 5,663,790 - AO353_25680 0.82 -1.2 5,663,790 - AO353_25680 0.82 -1.5 5,663,799 - AO353_25680 0.83 +1.0 5,663,966 - +1.1 5,663,966 - +1.1 5,663,983 + -0.4 5,663,999 + -0.1 5,663,999 + -0.1 5,663,999 + -2.7 5,663,999 + -0.5 5,663,999 + +0.3 5,664,000 - -0.8 5,664,002 + -1.0 5,664,003 - +0.6 5,664,562 + AO353_25685 0.47 -0.4 5,664,562 + AO353_25685 0.47 +0.5 5,664,562 + AO353_25685 0.47 +0.0 5,664,563 - AO353_25685 0.47 -0.4 5,664,563 - AO353_25685 0.47 -1.3 5,664,725 + AO353_25685 0.61 -2.3 5,664,726 - AO353_25685 0.61 +1.1 5,664,726 - AO353_25685 0.61 +0.1 5,664,726 - AO353_25685 0.61 -0.4 5,664,812 + AO353_25685 0.68 -1.7 5,664,812 + AO353_25685 0.68 -2.4 5,664,812 + AO353_25685 0.68 -0.9 5,664,812 + AO353_25685 0.68 -1.1 5,664,812 + AO353_25685 0.68 +1.1 5,664,812 + AO353_25685 0.68 +0.6 5,664,813 - AO353_25685 0.68 +1.1 5,664,813 - AO353_25685 0.68 -0.5 5,664,813 - AO353_25685 0.68 +1.7 5,664,813 - AO353_25685 0.68 +0.3 5,664,813 - AO353_25685 0.68 -0.1 5,664,813 - AO353_25685 0.68 +0.6 5,664,813 - AO353_25685 0.68 +2.5 5,664,813 - AO353_25685 0.68 -0.7 5,664,813 - AO353_25685 0.68 +2.0 5,664,813 - AO353_25685 0.68 +2.1 5,664,813 - AO353_25685 0.68 +0.3 5,664,813 - AO353_25685 0.68 +0.2 5,664,813 - AO353_25685 0.68 -0.3 5,664,813 - AO353_25685 0.68 +0.9 5,665,036 + AO353_25685 0.87 -0.0 5,665,036 + AO353_25685 0.87 -1.5 5,665,198 + +0.7 5,665,198 + +0.3 5,665,198 + +0.3 5,665,198 + +0.6 5,665,198 + -0.2 5,665,198 + -0.2 5,665,198 + +0.2 5,665,198 + +0.5 5,665,198 + -0.5 5,665,199 - -1.9 5,665,199 - +3.0 5,665,205 + +0.1 5,665,205 + +0.6 5,665,206 - -0.9 5,665,206 - +1.1 5,665,275 + -0.4 5,665,275 + +0.9 5,665,276 - -2.0 5,665,276 - -0.4 5,665,276 - +0.1 5,665,276 - -0.6 5,665,276 - +1.1 5,665,276 - +0.1 5,665,276 - -2.3 5,665,276 - +0.3 5,665,276 - -0.7 5,666,045 - -2.3 5,666,045 - -1.0 5,666,045 - -2.6
Or see this region's nucleotide sequence