Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_731 and Pf1N1B4_732 are separated by 55 nucleotides Pf1N1B4_732 and Pf1N1B4_733 are separated by 108 nucleotides Pf1N1B4_733 and Pf1N1B4_734 are separated by 129 nucleotides Pf1N1B4_734 and Pf1N1B4_735 overlap by 4 nucleotides
Pf1N1B4_731 - Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like, at 833,953 to 835,047
_731
Pf1N1B4_732 - hypothetical protein, at 835,103 to 835,306
_732
Pf1N1B4_733 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 835,415 to 837,466
_733
Pf1N1B4_734 - Electron transport complex protein RnfA, at 837,596 to 838,165
_734
Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169
_735
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 834.903 kb on - strand, within Pf1N1B4_731 at 834.903 kb on - strand, within Pf1N1B4_731 at 834.903 kb on - strand, within Pf1N1B4_731 at 835.090 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.099 kb on + strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.100 kb on - strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.106 kb on + strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.107 kb on - strand at 835.189 kb on + strand, within Pf1N1B4_732 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.030 kb on + strand, within Pf1N1B4_733 at 837.465 kb on - strand at 837.465 kb on - strand at 837.558 kb on + strand at 837.758 kb on + strand, within Pf1N1B4_734 at 837.768 kb on - strand, within Pf1N1B4_734 at 837.777 kb on + strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.778 kb on - strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.780 kb on + strand, within Pf1N1B4_734 at 837.781 kb on - strand, within Pf1N1B4_734 at 837.984 kb on + strand, within Pf1N1B4_734 at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.233 kb on + strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.234 kb on - strand at 838.241 kb on - strand at 838.241 kb on - strand at 838.384 kb on + strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735 at 838.385 kb on - strand, within Pf1N1B4_735
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 834,903 - Pf1N1B4_731 0.87 +0.4 834,903 - Pf1N1B4_731 0.87 +0.2 834,903 - Pf1N1B4_731 0.87 -2.3 835,090 + -0.1 835,099 + +0.7 835,099 + -1.1 835,099 + +0.7 835,099 + -1.4 835,099 + -2.6 835,099 + -3.3 835,099 + +1.7 835,099 + +0.7 835,099 + -2.3 835,100 - -1.0 835,100 - -2.4 835,100 - -0.5 835,100 - -1.1 835,100 - +0.7 835,100 - +0.7 835,100 - +0.7 835,100 - +0.7 835,106 + +0.7 835,106 + -0.3 835,106 + +0.7 835,106 + -3.1 835,106 + -1.6 835,107 - -4.4 835,107 - -0.3 835,107 - +0.7 835,107 - -2.6 835,107 - +0.7 835,107 - -1.3 835,107 - -1.8 835,189 + Pf1N1B4_732 0.42 +0.3 837,030 + Pf1N1B4_733 0.79 +0.9 837,030 + Pf1N1B4_733 0.79 -1.3 837,030 + Pf1N1B4_733 0.79 +0.7 837,465 - +0.7 837,465 - -0.6 837,558 + -1.3 837,758 + Pf1N1B4_734 0.28 +0.7 837,768 - Pf1N1B4_734 0.30 -1.6 837,777 + Pf1N1B4_734 0.32 -0.5 837,778 - Pf1N1B4_734 0.32 -1.0 837,778 - Pf1N1B4_734 0.32 +0.7 837,780 + Pf1N1B4_734 0.32 -0.3 837,780 + Pf1N1B4_734 0.32 +0.7 837,781 - Pf1N1B4_734 0.32 -1.9 837,984 + Pf1N1B4_734 0.68 -0.2 838,233 + +0.7 838,233 + +0.7 838,233 + +0.7 838,233 + +0.7 838,234 - +0.7 838,234 - +0.5 838,234 - +0.7 838,234 - +0.7 838,234 - -3.0 838,234 - +0.7 838,234 - -2.8 838,234 - +0.7 838,241 - +0.7 838,241 - -1.1 838,384 + Pf1N1B4_735 0.22 -1.5 838,385 - Pf1N1B4_735 0.22 -0.4 838,385 - Pf1N1B4_735 0.22 +0.7
Or see this region's nucleotide sequence