Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5912 and Pf1N1B4_5913 are separated by 151 nucleotides Pf1N1B4_5913 and Pf1N1B4_5914 are separated by 210 nucleotides Pf1N1B4_5914 and Pf1N1B4_5915 are separated by 225 nucleotides Pf1N1B4_5915 and Pf1N1B4_5916 are separated by 33 nucleotides
Pf1N1B4_5912 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 355,246 to 355,614
_5912
Pf1N1B4_5913 - hypothetical protein, at 355,766 to 355,930
_5913
Pf1N1B4_5914 - Transcriptional regulator, LysR family, at 356,141 to 357,064
_5914
Pf1N1B4_5915 - Transcriptional regulator, AraC family, at 357,290 to 357,757
_5915
Pf1N1B4_5916 - Similar to non-heme chloroperoxidase, sll5080 homolog, at 357,791 to 358,615
_5916
Position (kb) 356
357
358 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 355.341 kb on + strand, within Pf1N1B4_5912 at 355.342 kb on - strand, within Pf1N1B4_5912 at 355.342 kb on - strand, within Pf1N1B4_5912 at 355.427 kb on - strand, within Pf1N1B4_5912 at 355.427 kb on - strand, within Pf1N1B4_5912 at 355.460 kb on + strand at 355.565 kb on + strand, within Pf1N1B4_5912 at 355.565 kb on + strand, within Pf1N1B4_5912 at 355.631 kb on - strand at 355.631 kb on - strand at 355.668 kb on - strand at 355.668 kb on - strand at 355.706 kb on + strand at 355.706 kb on + strand at 355.706 kb on + strand at 355.707 kb on - strand at 355.707 kb on - strand at 355.707 kb on - strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.781 kb on + strand at 355.782 kb on - strand at 355.803 kb on + strand, within Pf1N1B4_5913 at 355.805 kb on + strand, within Pf1N1B4_5913 at 355.806 kb on - strand at 355.928 kb on + strand at 355.929 kb on - strand at 355.929 kb on - strand at 355.929 kb on - strand at 355.943 kb on - strand at 355.943 kb on - strand at 355.958 kb on + strand at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.322 kb on + strand, within Pf1N1B4_5914 at 356.598 kb on + strand, within Pf1N1B4_5914 at 356.652 kb on + strand, within Pf1N1B4_5914 at 356.652 kb on + strand, within Pf1N1B4_5914 at 356.652 kb on + strand, within Pf1N1B4_5914 at 356.653 kb on - strand, within Pf1N1B4_5914 at 357.065 kb on - strand at 357.065 kb on - strand at 357.153 kb on - strand at 357.163 kb on - strand at 357.629 kb on - strand at 357.747 kb on + strand at 357.747 kb on + strand at 357.748 kb on - strand at 357.755 kb on + strand at 357.871 kb on - strand at 357.998 kb on - strand at 358.033 kb on - strand, within Pf1N1B4_5916
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 355,341 + Pf1N1B4_5912 0.26 +0.0 355,342 - Pf1N1B4_5912 0.26 -2.2 355,342 - Pf1N1B4_5912 0.26 -1.5 355,427 - Pf1N1B4_5912 0.49 +0.5 355,427 - Pf1N1B4_5912 0.49 +0.5 355,460 + +0.5 355,565 + Pf1N1B4_5912 0.86 +0.5 355,565 + Pf1N1B4_5912 0.86 -0.6 355,631 - +0.3 355,631 - -0.7 355,668 - -0.6 355,668 - -1.0 355,706 + +2.1 355,706 + -1.9 355,706 + -0.5 355,707 - -2.8 355,707 - -1.7 355,707 - -0.6 355,708 + +0.5 355,708 + -2.7 355,708 + -2.7 355,708 + -0.2 355,781 + +1.0 355,782 - -0.1 355,803 + Pf1N1B4_5913 0.22 +0.8 355,805 + Pf1N1B4_5913 0.24 -1.1 355,806 - -1.1 355,928 + -3.1 355,929 - -2.9 355,929 - -1.8 355,929 - +0.5 355,943 - +0.5 355,943 - -1.3 355,958 + -2.3 356,293 + Pf1N1B4_5914 0.16 -0.5 356,293 + Pf1N1B4_5914 0.16 -1.2 356,293 + Pf1N1B4_5914 0.16 +0.5 356,293 + Pf1N1B4_5914 0.16 -2.7 356,293 + Pf1N1B4_5914 0.16 -2.1 356,322 + Pf1N1B4_5914 0.20 +0.1 356,598 + Pf1N1B4_5914 0.49 -1.4 356,652 + Pf1N1B4_5914 0.55 -1.7 356,652 + Pf1N1B4_5914 0.55 -3.2 356,652 + Pf1N1B4_5914 0.55 -1.4 356,653 - Pf1N1B4_5914 0.55 -2.8 357,065 - +1.7 357,065 - +0.4 357,153 - +0.3 357,163 - -2.3 357,629 - -1.1 357,747 + +0.5 357,747 + -4.4 357,748 - +0.1 357,755 + +0.6 357,871 - -0.9 357,998 - -1.1 358,033 - Pf1N1B4_5916 0.29 -2.3
Or see this region's nucleotide sequence