Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5606 and Pf1N1B4_5607 are separated by 557 nucleotides Pf1N1B4_5607 and Pf1N1B4_5608 are separated by 565 nucleotides
Pf1N1B4_5606 - Alpha-ketoglutarate permease, at 30,868 to 32,253
_5606
Pf1N1B4_5607 - C4-dicarboxylate transport protein, at 32,811 to 34,115
_5607
Pf1N1B4_5608 - Sulfopyruvate decarboxylase - alpha subunit (EC 4.1.1.79), at 34,681 to 35,220
_5608
Position (kb)
32
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1 at 31.942 kb on + strand, within Pf1N1B4_5606 at 32.006 kb on + strand, within Pf1N1B4_5606 at 32.006 kb on + strand, within Pf1N1B4_5606 at 32.007 kb on - strand, within Pf1N1B4_5606 at 32.056 kb on + strand, within Pf1N1B4_5606 at 32.057 kb on - strand, within Pf1N1B4_5606 at 32.107 kb on + strand, within Pf1N1B4_5606 at 32.107 kb on + strand at 32.108 kb on - strand, within Pf1N1B4_5606 at 32.108 kb on - strand at 32.176 kb on + strand at 32.177 kb on - strand at 32.259 kb on - strand at 32.345 kb on - strand at 32.529 kb on - strand at 32.729 kb on + strand at 32.730 kb on - strand at 33.123 kb on - strand, within Pf1N1B4_5607 at 33.182 kb on - strand, within Pf1N1B4_5607 at 33.182 kb on - strand, within Pf1N1B4_5607 at 33.212 kb on - strand, within Pf1N1B4_5607 at 33.464 kb on - strand, within Pf1N1B4_5607 at 33.967 kb on - strand, within Pf1N1B4_5607 at 33.967 kb on - strand at 33.995 kb on - strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.328 kb on + strand at 34.329 kb on - strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.332 kb on + strand at 34.333 kb on - strand at 34.333 kb on - strand at 34.352 kb on + strand at 34.354 kb on + strand at 34.354 kb on + strand at 34.355 kb on - strand at 34.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 31,942 + Pf1N1B4_5606 0.77 +0.4 32,006 + Pf1N1B4_5606 0.82 +0.6 32,006 + Pf1N1B4_5606 0.82 -0.7 32,007 - Pf1N1B4_5606 0.82 -1.5 32,056 + Pf1N1B4_5606 0.86 +0.3 32,057 - Pf1N1B4_5606 0.86 -0.2 32,107 + Pf1N1B4_5606 0.89 -0.1 32,107 + +0.4 32,108 - Pf1N1B4_5606 0.89 +0.4 32,108 - -2.1 32,176 + +0.9 32,177 - +1.0 32,259 - -2.1 32,345 - +0.4 32,529 - -0.3 32,729 + -0.5 32,730 - +0.7 33,123 - Pf1N1B4_5607 0.24 -0.1 33,182 - Pf1N1B4_5607 0.28 +0.4 33,182 - Pf1N1B4_5607 0.28 +0.1 33,212 - Pf1N1B4_5607 0.31 -1.6 33,464 - Pf1N1B4_5607 0.50 +0.1 33,967 - Pf1N1B4_5607 0.89 +0.2 33,967 - -1.3 33,995 - +0.7 34,328 + +0.4 34,328 + -0.6 34,328 + -2.1 34,328 + -1.3 34,329 - -1.3 34,332 + -2.4 34,332 + -0.7 34,332 + -2.4 34,332 + +0.4 34,332 + +0.6 34,332 + -1.3 34,332 + -1.4 34,332 + +1.1 34,332 + -2.1 34,332 + -1.1 34,332 + -1.9 34,332 + -0.6 34,333 - +0.4 34,333 - -0.9 34,352 + +0.4 34,354 + -1.1 34,354 + +0.4 34,355 - +1.4 34,403 - -0.2
Or see this region's nucleotide sequence