Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5469 and Pf1N1B4_5470 are separated by 489 nucleotides Pf1N1B4_5470 and Pf1N1B4_5471 are separated by 20 nucleotides
Pf1N1B4_5469 - hypothetical protein, at 220,007 to 220,381
_5469
Pf1N1B4_5470 - hypothetical protein, at 220,871 to 221,125
_5470
Pf1N1B4_5471 - hypothetical protein, at 221,146 to 221,352
_5471
Position (kb) 220
221
222 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.094 kb on + strand, within Pf1N1B4_5469 at 220.095 kb on - strand, within Pf1N1B4_5469 at 220.095 kb on - strand, within Pf1N1B4_5469 at 220.095 kb on - strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.096 kb on + strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.097 kb on - strand, within Pf1N1B4_5469 at 220.217 kb on + strand at 220.217 kb on + strand, within Pf1N1B4_5469 at 220.217 kb on + strand, within Pf1N1B4_5469 at 220.344 kb on + strand at 220.344 kb on + strand at 220.344 kb on + strand at 220.344 kb on + strand at 220.380 kb on - strand at 220.457 kb on + strand at 220.457 kb on + strand at 220.492 kb on + strand at 220.493 kb on - strand at 220.493 kb on - strand at 220.657 kb on + strand at 220.658 kb on - strand at 220.681 kb on + strand at 220.681 kb on + strand at 220.681 kb on + strand at 220.681 kb on + strand at 220.739 kb on + strand at 220.739 kb on + strand at 220.739 kb on + strand at 220.752 kb on + strand at 220.752 kb on + strand at 220.752 kb on + strand at 220.752 kb on + strand at 220.752 kb on + strand at 220.753 kb on - strand at 220.756 kb on + strand at 220.756 kb on + strand at 220.756 kb on + strand at 220.756 kb on + strand at 220.756 kb on + strand at 220.756 kb on + strand at 220.757 kb on - strand at 220.757 kb on - strand at 220.805 kb on + strand at 220.816 kb on - strand at 220.818 kb on + strand at 220.818 kb on + strand at 220.819 kb on - strand at 220.842 kb on + strand at 220.842 kb on + strand at 220.843 kb on - strand at 220.843 kb on - strand at 220.845 kb on + strand at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.986 kb on + strand, within Pf1N1B4_5470 at 220.987 kb on - strand, within Pf1N1B4_5470 at 220.987 kb on - strand, within Pf1N1B4_5470 at 220.987 kb on - strand at 220.991 kb on - strand at 221.139 kb on + strand at 221.139 kb on + strand at 221.332 kb on + strand at 221.332 kb on + strand at 221.332 kb on + strand at 221.332 kb on + strand at 221.333 kb on - strand at 221.333 kb on - strand at 221.333 kb on - strand at 221.333 kb on - strand at 221.371 kb on + strand at 221.371 kb on + strand at 221.372 kb on - strand at 221.417 kb on - strand at 221.538 kb on + strand at 221.538 kb on + strand at 221.648 kb on + strand at 221.682 kb on + strand at 221.780 kb on - strand at 221.780 kb on - strand at 221.780 kb on - strand at 221.834 kb on - strand at 221.835 kb on + strand at 221.835 kb on + strand at 221.835 kb on + strand at 221.835 kb on + strand at 221.915 kb on + strand at 221.915 kb on + strand at 221.941 kb on - strand at 222.062 kb on + strand at 222.062 kb on + strand at 222.063 kb on - strand at 222.063 kb on - strand at 222.063 kb on - strand at 222.063 kb on - strand at 222.073 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 220,094 + Pf1N1B4_5469 0.23 -0.9 220,094 + Pf1N1B4_5469 0.23 -0.9 220,094 + Pf1N1B4_5469 0.23 -0.1 220,094 + Pf1N1B4_5469 0.23 +0.8 220,094 + Pf1N1B4_5469 0.23 -0.8 220,094 + Pf1N1B4_5469 0.23 -0.2 220,094 + Pf1N1B4_5469 0.23 -1.8 220,095 - Pf1N1B4_5469 0.23 -1.8 220,095 - Pf1N1B4_5469 0.23 +0.8 220,095 - Pf1N1B4_5469 0.23 -2.2 220,096 + Pf1N1B4_5469 0.24 -0.7 220,096 + Pf1N1B4_5469 0.24 +0.8 220,096 + Pf1N1B4_5469 0.24 +0.8 220,096 + Pf1N1B4_5469 0.24 -2.0 220,096 + Pf1N1B4_5469 0.24 +0.8 220,096 + Pf1N1B4_5469 0.24 -1.5 220,096 + Pf1N1B4_5469 0.24 -0.8 220,096 + Pf1N1B4_5469 0.24 -1.9 220,096 + Pf1N1B4_5469 0.24 -2.9 220,096 + +0.8 220,096 + Pf1N1B4_5469 0.24 +0.8 220,096 + Pf1N1B4_5469 0.24 -0.5 220,096 + Pf1N1B4_5469 0.24 +2.4 220,096 + Pf1N1B4_5469 0.24 +0.8 220,096 + Pf1N1B4_5469 0.24 -2.2 220,096 + Pf1N1B4_5469 0.24 -2.2 220,096 + Pf1N1B4_5469 0.24 -2.4 220,096 + Pf1N1B4_5469 0.24 +0.7 220,097 - Pf1N1B4_5469 0.24 +0.8 220,097 - Pf1N1B4_5469 0.24 -1.0 220,097 - Pf1N1B4_5469 0.24 +0.8 220,097 - Pf1N1B4_5469 0.24 -1.6 220,097 - Pf1N1B4_5469 0.24 -1.1 220,097 - Pf1N1B4_5469 0.24 -0.7 220,097 - Pf1N1B4_5469 0.24 -1.4 220,217 + -1.0 220,217 + Pf1N1B4_5469 0.56 +0.8 220,217 + Pf1N1B4_5469 0.56 +1.1 220,344 + +2.0 220,344 + +0.8 220,344 + +0.5 220,344 + +1.9 220,380 - -0.2 220,457 + -1.8 220,457 + -1.8 220,492 + -1.0 220,493 - +0.8 220,493 - -2.5 220,657 + -0.4 220,658 - -1.1 220,681 + -0.6 220,681 + +0.5 220,681 + +0.8 220,681 + +0.8 220,739 + +0.6 220,739 + -0.2 220,739 + -3.4 220,752 + -0.4 220,752 + +0.8 220,752 + -0.5 220,752 + -2.4 220,752 + +0.8 220,753 - -1.4 220,756 + +1.1 220,756 + -0.2 220,756 + +0.1 220,756 + -3.6 220,756 + -0.3 220,756 + -1.5 220,757 - -0.8 220,757 - -2.4 220,805 + -1.0 220,816 - +0.8 220,818 + -2.8 220,818 + -0.4 220,819 - -3.3 220,842 + +1.7 220,842 + -0.0 220,843 - +0.8 220,843 - -0.7 220,845 + -0.0 220,986 + Pf1N1B4_5470 0.45 -2.8 220,986 + Pf1N1B4_5470 0.45 -3.3 220,986 + Pf1N1B4_5470 0.45 -2.2 220,986 + Pf1N1B4_5470 0.45 -1.5 220,986 + Pf1N1B4_5470 0.45 +0.8 220,986 + Pf1N1B4_5470 0.45 -4.2 220,986 + Pf1N1B4_5470 0.45 -0.2 220,986 + Pf1N1B4_5470 0.45 -2.2 220,986 + Pf1N1B4_5470 0.45 -0.8 220,987 - Pf1N1B4_5470 0.45 -0.8 220,987 - Pf1N1B4_5470 0.45 +0.8 220,987 - -2.0 220,991 - +0.2 221,139 + -0.4 221,139 + +0.8 221,332 + -1.1 221,332 + +2.5 221,332 + -1.3 221,332 + +0.8 221,333 - -0.1 221,333 - -1.8 221,333 - +0.8 221,333 - -0.3 221,371 + -1.5 221,371 + -0.8 221,372 - -2.3 221,417 - -1.5 221,538 + -3.0 221,538 + -1.1 221,648 + -0.2 221,682 + +2.4 221,780 - +0.8 221,780 - -2.2 221,780 - -0.4 221,834 - -3.4 221,835 + -0.0 221,835 + +0.8 221,835 + -0.8 221,835 + -1.5 221,915 + -0.0 221,915 + +0.0 221,941 - -0.2 222,062 + +0.8 222,062 + +0.8 222,063 - -1.0 222,063 - -2.0 222,063 - -2.9 222,063 - +0.4 222,073 + -0.9
Or see this region's nucleotide sequence