Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5415

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5414 and Pf1N1B4_5415 are separated by 3 nucleotidesPf1N1B4_5415 and Pf1N1B4_5416 are separated by 95 nucleotidesPf1N1B4_5416 and Pf1N1B4_5417 are separated by 11 nucleotides Pf1N1B4_5414 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 152,475 to 155,366 _5414 Pf1N1B4_5415 - hypothetical protein, at 155,370 to 155,732 _5415 Pf1N1B4_5416 - hypothetical protein, at 155,828 to 156,166 _5416 Pf1N1B4_5417 - hypothetical protein, at 156,178 to 157,722 _5417 Position (kb) 155 156Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 154.370 kb on - strandat 154.492 kb on + strand, within Pf1N1B4_5414at 154.917 kb on + strand, within Pf1N1B4_5414at 155.050 kb on + strand, within Pf1N1B4_5414at 155.158 kb on + strandat 155.343 kb on + strandat 155.343 kb on + strandat 155.344 kb on - strandat 155.344 kb on - strandat 155.344 kb on - strandat 155.414 kb on - strand, within Pf1N1B4_5415at 155.432 kb on + strandat 155.449 kb on + strand, within Pf1N1B4_5415at 155.511 kb on + strand, within Pf1N1B4_5415at 155.524 kb on + strand, within Pf1N1B4_5415at 155.524 kb on + strand, within Pf1N1B4_5415at 155.524 kb on + strand, within Pf1N1B4_5415at 155.524 kb on + strand, within Pf1N1B4_5415at 155.524 kb on + strand, within Pf1N1B4_5415at 155.549 kb on + strand, within Pf1N1B4_5415at 155.549 kb on + strand, within Pf1N1B4_5415at 155.628 kb on + strand, within Pf1N1B4_5415at 155.628 kb on + strand, within Pf1N1B4_5415at 155.629 kb on - strand, within Pf1N1B4_5415at 155.804 kb on + strandat 155.805 kb on - strandat 155.805 kb on - strandat 155.805 kb on - strandat 155.825 kb on + strandat 155.825 kb on + strandat 155.826 kb on - strandat 155.826 kb on - strandat 155.829 kb on + strandat 155.830 kb on - strandat 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.862 kb on + strand, within Pf1N1B4_5416at 155.863 kb on - strandat 155.930 kb on + strand, within Pf1N1B4_5416at 155.931 kb on - strand, within Pf1N1B4_5416at 155.958 kb on + strand, within Pf1N1B4_5416at 155.958 kb on + strand, within Pf1N1B4_5416at 155.958 kb on + strand, within Pf1N1B4_5416at 155.958 kb on + strand, within Pf1N1B4_5416at 155.959 kb on - strand, within Pf1N1B4_5416at 155.959 kb on - strand, within Pf1N1B4_5416at 156.051 kb on + strand, within Pf1N1B4_5416at 156.051 kb on + strand, within Pf1N1B4_5416at 156.051 kb on + strand, within Pf1N1B4_5416at 156.064 kb on + strand, within Pf1N1B4_5416at 156.064 kb on + strand, within Pf1N1B4_5416at 156.064 kb on + strand, within Pf1N1B4_5416at 156.065 kb on - strand, within Pf1N1B4_5416at 156.065 kb on - strandat 156.065 kb on - strand, within Pf1N1B4_5416at 156.065 kb on - strand, within Pf1N1B4_5416at 156.065 kb on - strand, within Pf1N1B4_5416at 156.148 kb on + strandat 156.180 kb on - strandat 156.367 kb on + strand, within Pf1N1B4_5417at 156.367 kb on + strandat 156.367 kb on + strand, within Pf1N1B4_5417at 156.368 kb on - strand, within Pf1N1B4_5417at 156.393 kb on + strand, within Pf1N1B4_5417at 156.394 kb on - strand, within Pf1N1B4_5417at 156.405 kb on + strandat 156.476 kb on + strand, within Pf1N1B4_5417at 156.476 kb on + strand, within Pf1N1B4_5417at 156.545 kb on + strand, within Pf1N1B4_5417at 156.545 kb on + strand, within Pf1N1B4_5417at 156.545 kb on + strand, within Pf1N1B4_5417at 156.546 kb on - strand, within Pf1N1B4_5417at 156.581 kb on + strand, within Pf1N1B4_5417at 156.581 kb on + strand, within Pf1N1B4_5417at 156.581 kb on + strand, within Pf1N1B4_5417at 156.582 kb on - strand, within Pf1N1B4_5417

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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154,370 - -1.8
154,492 + Pf1N1B4_5414 0.70 -0.2
154,917 + Pf1N1B4_5414 0.84 +0.8
155,050 + Pf1N1B4_5414 0.89 -0.8
155,158 + -0.3
155,343 + -0.9
155,343 + +0.0
155,344 - -1.1
155,344 - +0.8
155,344 - +0.6
155,414 - Pf1N1B4_5415 0.12 -0.2
155,432 + -0.8
155,449 + Pf1N1B4_5415 0.22 -1.8
155,511 + Pf1N1B4_5415 0.39 -0.2
155,524 + Pf1N1B4_5415 0.42 +0.3
155,524 + Pf1N1B4_5415 0.42 +0.8
155,524 + Pf1N1B4_5415 0.42 +0.8
155,524 + Pf1N1B4_5415 0.42 +1.0
155,524 + Pf1N1B4_5415 0.42 +0.8
155,549 + Pf1N1B4_5415 0.49 +0.8
155,549 + Pf1N1B4_5415 0.49 -1.2
155,628 + Pf1N1B4_5415 0.71 -0.2
155,628 + Pf1N1B4_5415 0.71 -1.5
155,629 - Pf1N1B4_5415 0.71 -1.0
155,804 + -1.4
155,805 - -0.2
155,805 - +0.8
155,805 - -0.8
155,825 + +0.8
155,825 + -1.0
155,826 - -3.4
155,826 - -0.8
155,829 + -1.9
155,830 - +0.8
155,862 + Pf1N1B4_5416 0.10 -0.5
155,862 + Pf1N1B4_5416 0.10 +0.4
155,862 + Pf1N1B4_5416 0.10 -1.2
155,862 + Pf1N1B4_5416 0.10 +0.8
155,862 + Pf1N1B4_5416 0.10 -1.0
155,862 + Pf1N1B4_5416 0.10 -0.1
155,862 + Pf1N1B4_5416 0.10 -0.1
155,863 - +0.8
155,930 + Pf1N1B4_5416 0.30 -0.2
155,931 - Pf1N1B4_5416 0.30 -0.3
155,958 + Pf1N1B4_5416 0.38 -0.9
155,958 + Pf1N1B4_5416 0.38 +0.6
155,958 + Pf1N1B4_5416 0.38 -0.2
155,958 + Pf1N1B4_5416 0.38 -3.0
155,959 - Pf1N1B4_5416 0.39 -0.9
155,959 - Pf1N1B4_5416 0.39 +0.8
156,051 + Pf1N1B4_5416 0.66 +0.2
156,051 + Pf1N1B4_5416 0.66 +2.8
156,051 + Pf1N1B4_5416 0.66 -0.6
156,064 + Pf1N1B4_5416 0.70 -1.4
156,064 + Pf1N1B4_5416 0.70 -3.1
156,064 + Pf1N1B4_5416 0.70 +0.8
156,065 - Pf1N1B4_5416 0.70 -1.5
156,065 - +0.8
156,065 - Pf1N1B4_5416 0.70 -0.8
156,065 - Pf1N1B4_5416 0.70 +1.8
156,065 - Pf1N1B4_5416 0.70 +0.2
156,148 + +0.8
156,180 - -3.1
156,367 + Pf1N1B4_5417 0.12 -2.5
156,367 + +0.8
156,367 + Pf1N1B4_5417 0.12 -0.9
156,368 - Pf1N1B4_5417 0.12 -1.2
156,393 + Pf1N1B4_5417 0.14 -1.2
156,394 - Pf1N1B4_5417 0.14 -1.8
156,405 + -0.5
156,476 + Pf1N1B4_5417 0.19 +0.8
156,476 + Pf1N1B4_5417 0.19 +0.8
156,545 + Pf1N1B4_5417 0.24 -0.2
156,545 + Pf1N1B4_5417 0.24 +0.4
156,545 + Pf1N1B4_5417 0.24 -0.0
156,546 - Pf1N1B4_5417 0.24 -1.1
156,581 + Pf1N1B4_5417 0.26 +0.8
156,581 + Pf1N1B4_5417 0.26 -0.3
156,581 + Pf1N1B4_5417 0.26 +1.3
156,582 - Pf1N1B4_5417 0.26 -0.3

Or see this region's nucleotide sequence