Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5366 and Pf1N1B4_5367 are separated by 291 nucleotides Pf1N1B4_5367 and Pf1N1B4_5368 are separated by 16 nucleotides Pf1N1B4_5368 and Pf1N1B4_5369 are separated by 470 nucleotides
Pf1N1B4_5366 - hypothetical protein, at 99,603 to 99,803
_5366
Pf1N1B4_5367 - hypothetical protein, at 100,095 to 100,583
_5367
Pf1N1B4_5368 - hypothetical protein, at 100,600 to 100,914
_5368
Pf1N1B4_5369 - hypothetical protein, at 101,385 to 101,894
_5369
Position (kb)
100
101 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 99.746 kb on + strand, within Pf1N1B4_5366 at 99.895 kb on + strand at 99.896 kb on - strand at 99.896 kb on - strand at 99.896 kb on - strand at 99.896 kb on - strand at 100.062 kb on + strand at 100.077 kb on + strand at 100.077 kb on + strand at 100.077 kb on + strand at 100.174 kb on + strand, within Pf1N1B4_5367 at 100.249 kb on + strand, within Pf1N1B4_5367 at 100.273 kb on + strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.314 kb on - strand, within Pf1N1B4_5367 at 100.468 kb on + strand, within Pf1N1B4_5367 at 100.468 kb on + strand, within Pf1N1B4_5367 at 100.534 kb on + strand, within Pf1N1B4_5367 at 100.534 kb on + strand, within Pf1N1B4_5367 at 100.534 kb on + strand, within Pf1N1B4_5367 at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.587 kb on + strand at 100.588 kb on - strand at 100.588 kb on - strand at 100.591 kb on + strand at 100.602 kb on - strand at 100.718 kb on + strand, within Pf1N1B4_5368 at 100.718 kb on + strand, within Pf1N1B4_5368 at 100.718 kb on + strand, within Pf1N1B4_5368 at 100.719 kb on - strand, within Pf1N1B4_5368 at 100.755 kb on - strand, within Pf1N1B4_5368 at 100.788 kb on + strand, within Pf1N1B4_5368 at 100.788 kb on + strand, within Pf1N1B4_5368 at 100.789 kb on - strand, within Pf1N1B4_5368 at 100.789 kb on - strand, within Pf1N1B4_5368 at 100.790 kb on + strand, within Pf1N1B4_5368 at 100.791 kb on - strand, within Pf1N1B4_5368 at 100.832 kb on + strand, within Pf1N1B4_5368 at 100.832 kb on + strand, within Pf1N1B4_5368 at 100.832 kb on + strand at 100.832 kb on + strand, within Pf1N1B4_5368 at 100.832 kb on + strand, within Pf1N1B4_5368 at 100.832 kb on + strand, within Pf1N1B4_5368 at 100.833 kb on - strand, within Pf1N1B4_5368 at 100.833 kb on - strand, within Pf1N1B4_5368 at 100.916 kb on + strand at 100.916 kb on + strand at 100.916 kb on + strand at 100.916 kb on + strand at 100.917 kb on - strand at 100.917 kb on - strand at 100.917 kb on - strand at 101.118 kb on + strand at 101.118 kb on + strand at 101.118 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.152 kb on - strand at 101.152 kb on - strand at 101.437 kb on + strand, within Pf1N1B4_5369 at 101.503 kb on + strand, within Pf1N1B4_5369 at 101.504 kb on - strand, within Pf1N1B4_5369 at 101.504 kb on - strand, within Pf1N1B4_5369 at 101.504 kb on - strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.506 kb on + strand, within Pf1N1B4_5369 at 101.507 kb on - strand, within Pf1N1B4_5369 at 101.507 kb on - strand, within Pf1N1B4_5369 at 101.507 kb on - strand, within Pf1N1B4_5369 at 101.508 kb on + strand, within Pf1N1B4_5369 at 101.509 kb on - strand at 101.509 kb on - strand, within Pf1N1B4_5369 at 101.509 kb on - strand, within Pf1N1B4_5369 at 101.542 kb on + strand, within Pf1N1B4_5369 at 101.542 kb on + strand at 101.542 kb on + strand, within Pf1N1B4_5369 at 101.543 kb on - strand, within Pf1N1B4_5369 at 101.543 kb on - strand, within Pf1N1B4_5369 at 101.595 kb on + strand, within Pf1N1B4_5369 at 101.595 kb on + strand, within Pf1N1B4_5369 at 101.595 kb on + strand, within Pf1N1B4_5369 at 101.595 kb on + strand, within Pf1N1B4_5369 at 101.596 kb on - strand, within Pf1N1B4_5369 at 101.596 kb on - strand, within Pf1N1B4_5369 at 101.596 kb on - strand, within Pf1N1B4_5369 at 101.596 kb on - strand, within Pf1N1B4_5369 at 101.598 kb on + strand, within Pf1N1B4_5369 at 101.598 kb on + strand, within Pf1N1B4_5369 at 101.598 kb on + strand, within Pf1N1B4_5369 at 101.599 kb on - strand, within Pf1N1B4_5369 at 101.802 kb on + strand, within Pf1N1B4_5369 at 101.802 kb on + strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.803 kb on - strand, within Pf1N1B4_5369 at 101.857 kb on + strand at 101.857 kb on + strand at 101.858 kb on - strand at 101.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 99,746 + Pf1N1B4_5366 0.71 -3.2 99,895 + -1.6 99,896 - -1.2 99,896 - -1.3 99,896 - -1.8 99,896 - +0.8 100,062 + -0.7 100,077 + -0.4 100,077 + -1.2 100,077 + -0.7 100,174 + Pf1N1B4_5367 0.16 +0.8 100,249 + Pf1N1B4_5367 0.31 -2.1 100,273 + Pf1N1B4_5367 0.36 -1.0 100,274 - Pf1N1B4_5367 0.37 -1.4 100,274 - Pf1N1B4_5367 0.37 -0.4 100,274 - Pf1N1B4_5367 0.37 -2.5 100,313 + Pf1N1B4_5367 0.45 -2.2 100,313 + Pf1N1B4_5367 0.45 -1.0 100,313 + Pf1N1B4_5367 0.45 -0.8 100,313 + Pf1N1B4_5367 0.45 -2.0 100,313 + Pf1N1B4_5367 0.45 +0.8 100,314 - Pf1N1B4_5367 0.45 +0.8 100,468 + Pf1N1B4_5367 0.76 -1.2 100,468 + Pf1N1B4_5367 0.76 +0.8 100,534 + Pf1N1B4_5367 0.90 -1.8 100,534 + Pf1N1B4_5367 0.90 +0.8 100,534 + Pf1N1B4_5367 0.90 +0.7 100,587 + -0.9 100,587 + -2.3 100,587 + -0.7 100,587 + +0.8 100,587 + -3.0 100,587 + -2.6 100,587 + -0.3 100,587 + +0.7 100,587 + +2.8 100,587 + -1.0 100,588 - -0.0 100,588 - -2.8 100,591 + -1.8 100,602 - -0.4 100,718 + Pf1N1B4_5368 0.37 +0.5 100,718 + Pf1N1B4_5368 0.37 +0.9 100,718 + Pf1N1B4_5368 0.37 -1.5 100,719 - Pf1N1B4_5368 0.38 +0.1 100,755 - Pf1N1B4_5368 0.49 +0.8 100,788 + Pf1N1B4_5368 0.60 +0.9 100,788 + Pf1N1B4_5368 0.60 -2.0 100,789 - Pf1N1B4_5368 0.60 +0.8 100,789 - Pf1N1B4_5368 0.60 -0.7 100,790 + Pf1N1B4_5368 0.60 -0.4 100,791 - Pf1N1B4_5368 0.61 -0.3 100,832 + Pf1N1B4_5368 0.74 -2.3 100,832 + Pf1N1B4_5368 0.74 +1.8 100,832 + +1.1 100,832 + Pf1N1B4_5368 0.74 -2.0 100,832 + Pf1N1B4_5368 0.74 +0.2 100,832 + Pf1N1B4_5368 0.74 +0.8 100,833 - Pf1N1B4_5368 0.74 -0.0 100,833 - Pf1N1B4_5368 0.74 -4.0 100,916 + -0.1 100,916 + +0.8 100,916 + -0.3 100,916 + +0.2 100,917 - +0.8 100,917 - +0.8 100,917 - +0.8 101,118 + -0.2 101,118 + +0.8 101,118 + +0.8 101,149 + +0.6 101,149 + +1.0 101,149 + -2.8 101,149 + +0.8 101,149 + -2.8 101,149 + -2.6 101,149 + +0.8 101,149 + -1.6 101,152 - -2.0 101,152 - +0.8 101,437 + Pf1N1B4_5369 0.10 -2.3 101,503 + Pf1N1B4_5369 0.23 -1.3 101,504 - Pf1N1B4_5369 0.23 -0.0 101,504 - Pf1N1B4_5369 0.23 -2.0 101,504 - Pf1N1B4_5369 0.23 -1.7 101,506 + Pf1N1B4_5369 0.24 -1.3 101,506 + Pf1N1B4_5369 0.24 +0.8 101,506 + Pf1N1B4_5369 0.24 -0.8 101,506 + +1.8 101,506 + Pf1N1B4_5369 0.24 -1.8 101,506 + Pf1N1B4_5369 0.24 +0.8 101,506 + Pf1N1B4_5369 0.24 -0.6 101,506 + Pf1N1B4_5369 0.24 +0.2 101,506 + Pf1N1B4_5369 0.24 +1.4 101,506 + Pf1N1B4_5369 0.24 -0.1 101,507 - Pf1N1B4_5369 0.24 +0.8 101,507 - Pf1N1B4_5369 0.24 +0.8 101,507 - Pf1N1B4_5369 0.24 +0.1 101,508 + Pf1N1B4_5369 0.24 +2.0 101,509 - -1.1 101,509 - Pf1N1B4_5369 0.24 -2.1 101,509 - Pf1N1B4_5369 0.24 +0.8 101,542 + Pf1N1B4_5369 0.31 +0.8 101,542 + +0.8 101,542 + Pf1N1B4_5369 0.31 -3.2 101,543 - Pf1N1B4_5369 0.31 -0.7 101,543 - Pf1N1B4_5369 0.31 -1.5 101,595 + Pf1N1B4_5369 0.41 -1.0 101,595 + Pf1N1B4_5369 0.41 -2.0 101,595 + Pf1N1B4_5369 0.41 +0.8 101,595 + Pf1N1B4_5369 0.41 -0.7 101,596 - Pf1N1B4_5369 0.41 -0.0 101,596 - Pf1N1B4_5369 0.41 -0.9 101,596 - Pf1N1B4_5369 0.41 +0.8 101,596 - Pf1N1B4_5369 0.41 -3.2 101,598 + Pf1N1B4_5369 0.42 -1.3 101,598 + Pf1N1B4_5369 0.42 -0.2 101,598 + Pf1N1B4_5369 0.42 -3.3 101,599 - Pf1N1B4_5369 0.42 +2.8 101,802 + Pf1N1B4_5369 0.82 -0.2 101,802 + Pf1N1B4_5369 0.82 -2.2 101,803 - Pf1N1B4_5369 0.82 +0.8 101,803 - Pf1N1B4_5369 0.82 -2.2 101,803 - Pf1N1B4_5369 0.82 -0.5 101,803 - Pf1N1B4_5369 0.82 +0.8 101,803 - Pf1N1B4_5369 0.82 -2.6 101,803 - Pf1N1B4_5369 0.82 +0.2 101,803 - Pf1N1B4_5369 0.82 -1.2 101,857 + -2.3 101,857 + +0.2 101,858 - +0.6 101,858 - -0.5
Or see this region's nucleotide sequence