Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5269

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5268 and Pf1N1B4_5269 are separated by 330 nucleotidesPf1N1B4_5269 and Pf1N1B4_5270 are separated by 604 nucleotides Pf1N1B4_5268 - hypothetical protein, at 25,619 to 26,140 _5268 Pf1N1B4_5269 - hypothetical protein, at 26,471 to 26,764 _5269 Pf1N1B4_5270 - hypothetical protein, at 27,369 to 28,193 _5270 Position (kb) 26 27Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 25.476 kb on + strandat 25.575 kb on + strandat 25.841 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.850 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.979 kb on + strand, within Pf1N1B4_5268at 25.989 kb on + strand, within Pf1N1B4_5268at 25.990 kb on - strand, within Pf1N1B4_5268at 25.990 kb on - strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.054 kb on + strand, within Pf1N1B4_5268at 26.055 kb on - strand, within Pf1N1B4_5268at 26.058 kb on + strandat 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.058 kb on + strand, within Pf1N1B4_5268at 26.059 kb on - strand, within Pf1N1B4_5268at 26.059 kb on - strand, within Pf1N1B4_5268at 26.128 kb on - strandat 26.128 kb on - strandat 26.166 kb on + strandat 26.166 kb on + strandat 26.167 kb on - strandat 26.189 kb on + strandat 26.189 kb on + strandat 26.190 kb on - strandat 26.190 kb on - strandat 26.227 kb on - strandat 26.348 kb on + strandat 26.348 kb on + strandat 26.382 kb on - strandat 26.385 kb on + strandat 26.386 kb on - strandat 26.521 kb on + strand, within Pf1N1B4_5269at 26.521 kb on + strand, within Pf1N1B4_5269at 26.521 kb on + strand, within Pf1N1B4_5269at 26.522 kb on - strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.660 kb on + strand, within Pf1N1B4_5269at 26.661 kb on - strand, within Pf1N1B4_5269at 26.837 kb on - strandat 26.980 kb on - strandat 26.980 kb on - strandat 26.980 kb on - strandat 27.045 kb on + strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.046 kb on - strandat 27.145 kb on + strandat 27.146 kb on - strandat 27.181 kb on + strandat 27.182 kb on - strandat 27.190 kb on - strandat 27.190 kb on - strandat 27.190 kb on - strandat 27.238 kb on - strandat 27.269 kb on + strandat 27.294 kb on - strandat 27.332 kb on - strandat 27.365 kb on + strandat 27.366 kb on - strandat 27.433 kb on - strandat 27.493 kb on + strand, within Pf1N1B4_5270at 27.500 kb on + strandat 27.594 kb on + strand, within Pf1N1B4_5270at 27.595 kb on - strandat 27.613 kb on - strand, within Pf1N1B4_5270at 27.637 kb on - strand, within Pf1N1B4_5270at 27.672 kb on + strand, within Pf1N1B4_5270at 27.693 kb on + strandat 27.694 kb on - strand, within Pf1N1B4_5270at 27.694 kb on - strand, within Pf1N1B4_5270at 27.746 kb on + strand, within Pf1N1B4_5270at 27.746 kb on + strand, within Pf1N1B4_5270at 27.747 kb on - strand, within Pf1N1B4_5270at 27.759 kb on - strand, within Pf1N1B4_5270at 27.759 kb on - strand, within Pf1N1B4_5270

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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25,476 + +0.8
25,575 + -1.2
25,841 + Pf1N1B4_5268 0.43 +0.8
25,850 + Pf1N1B4_5268 0.44 -1.2
25,850 + Pf1N1B4_5268 0.44 -0.4
25,850 + Pf1N1B4_5268 0.44 +0.8
25,850 + Pf1N1B4_5268 0.44 -0.9
25,979 + Pf1N1B4_5268 0.69 +0.8
25,979 + Pf1N1B4_5268 0.69 -0.9
25,979 + Pf1N1B4_5268 0.69 -0.5
25,989 + Pf1N1B4_5268 0.71 -2.2
25,990 - Pf1N1B4_5268 0.71 +0.2
25,990 - Pf1N1B4_5268 0.71 +2.1
26,054 + Pf1N1B4_5268 0.83 +0.8
26,054 + Pf1N1B4_5268 0.83 -0.3
26,054 + Pf1N1B4_5268 0.83 +0.4
26,055 - Pf1N1B4_5268 0.84 +0.3
26,058 + +0.8
26,058 + Pf1N1B4_5268 0.84 +0.8
26,058 + Pf1N1B4_5268 0.84 -0.7
26,058 + Pf1N1B4_5268 0.84 +0.8
26,058 + Pf1N1B4_5268 0.84 +0.8
26,058 + Pf1N1B4_5268 0.84 -0.6
26,059 - Pf1N1B4_5268 0.84 +0.1
26,059 - Pf1N1B4_5268 0.84 -0.2
26,128 - +0.3
26,128 - -1.3
26,166 + -1.5
26,166 + -3.0
26,167 - -0.0
26,189 + -0.0
26,189 + +0.1
26,190 - -3.0
26,190 - -0.8
26,227 - +0.8
26,348 + +2.0
26,348 + +0.8
26,382 - -1.9
26,385 + -1.3
26,386 - -0.9
26,521 + Pf1N1B4_5269 0.17 +2.2
26,521 + Pf1N1B4_5269 0.17 -0.2
26,521 + Pf1N1B4_5269 0.17 +0.8
26,522 - Pf1N1B4_5269 0.17 -0.4
26,660 + Pf1N1B4_5269 0.64 +0.8
26,660 + Pf1N1B4_5269 0.64 -1.2
26,660 + Pf1N1B4_5269 0.64 +0.6
26,661 - Pf1N1B4_5269 0.65 -0.2
26,837 - -0.8
26,980 - +0.8
26,980 - +0.8
26,980 - +0.6
27,045 + +0.1
27,046 - +0.8
27,046 - +0.8
27,046 - +0.8
27,046 - -1.0
27,046 - -0.4
27,145 + -0.5
27,146 - -3.0
27,181 + -2.2
27,182 - +0.2
27,190 - -2.2
27,190 - -0.4
27,190 - -0.7
27,238 - +0.8
27,269 + -1.7
27,294 - -2.0
27,332 - +0.8
27,365 + -2.3
27,366 - -0.3
27,433 - +0.8
27,493 + Pf1N1B4_5270 0.15 -1.6
27,500 + +0.8
27,594 + Pf1N1B4_5270 0.27 -0.6
27,595 - +1.1
27,613 - Pf1N1B4_5270 0.30 +0.8
27,637 - Pf1N1B4_5270 0.32 +1.6
27,672 + Pf1N1B4_5270 0.37 -0.0
27,693 + +0.1
27,694 - Pf1N1B4_5270 0.39 +0.8
27,694 - Pf1N1B4_5270 0.39 +0.6
27,746 + Pf1N1B4_5270 0.46 -1.8
27,746 + Pf1N1B4_5270 0.46 -1.5
27,747 - Pf1N1B4_5270 0.46 +0.8
27,759 - Pf1N1B4_5270 0.47 -2.0
27,759 - Pf1N1B4_5270 0.47 +0.2

Or see this region's nucleotide sequence