Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5263

Experiment: N2E2wt with N1B4m denitrifying repB

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf1N1B4_5262 and Pf1N1B4_5263 are separated by 491 nucleotidesPf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotidesPf1N1B4_5264 and Pf1N1B4_5265 are separated by 299 nucleotidesPf1N1B4_5265 and Pf1N1B4_5266 are separated by 21 nucleotides Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034 _5262 Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723 _5263 Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868 _5264 Pf1N1B4_5265 - hypothetical protein, at 23,168 to 23,296 _5265 Pf1N1B4_5266 - hypothetical protein, at 23,318 to 23,638 _5266 Position (kb) 22 23Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 21.589 kb on + strandat 21.724 kb on + strand, within Pf1N1B4_5262at 21.725 kb on - strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.904 kb on + strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.905 kb on - strand, within Pf1N1B4_5262at 21.941 kb on + strand, within Pf1N1B4_5262at 21.941 kb on + strand, within Pf1N1B4_5262at 21.943 kb on + strand, within Pf1N1B4_5262at 21.944 kb on - strand, within Pf1N1B4_5262at 21.944 kb on - strand, within Pf1N1B4_5262at 21.961 kb on + strand, within Pf1N1B4_5262at 22.078 kb on - strandat 22.104 kb on + strandat 22.105 kb on - strandat 22.105 kb on - strandat 22.159 kb on + strandat 22.159 kb on + strandat 22.160 kb on - strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.162 kb on + strandat 22.527 kb on + strandat 22.528 kb on - strandat 22.541 kb on + strandat 22.541 kb on + strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.542 kb on - strandat 22.604 kb on + strand, within Pf1N1B4_5263at 22.604 kb on + strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.605 kb on - strand, within Pf1N1B4_5263at 22.670 kb on + strand, within Pf1N1B4_5263at 22.671 kb on - strand, within Pf1N1B4_5263at 22.671 kb on - strandat 22.671 kb on - strandat 22.671 kb on - strand, within Pf1N1B4_5263at 22.718 kb on - strandat 22.718 kb on - strandat 22.797 kb on + strand, within Pf1N1B4_5264at 22.798 kb on - strandat 22.878 kb on + strandat 22.879 kb on - strandat 22.879 kb on - strandat 22.905 kb on - strandat 22.906 kb on + strandat 22.907 kb on - strandat 22.907 kb on - strandat 22.938 kb on - strandat 22.958 kb on - strandat 22.974 kb on - strandat 23.042 kb on - strandat 23.042 kb on - strandat 23.042 kb on - strandat 23.042 kb on - strandat 23.042 kb on - strandat 23.077 kb on - strandat 23.077 kb on - strandat 23.211 kb on - strand, within Pf1N1B4_5265at 23.211 kb on - strand, within Pf1N1B4_5265at 23.211 kb on - strand, within Pf1N1B4_5265at 23.251 kb on + strand, within Pf1N1B4_5265at 23.252 kb on - strand, within Pf1N1B4_5265at 23.252 kb on - strand, within Pf1N1B4_5265at 23.252 kb on - strand, within Pf1N1B4_5265at 23.252 kb on - strand, within Pf1N1B4_5265at 23.252 kb on - strand, within Pf1N1B4_5265at 23.312 kb on + strandat 23.312 kb on + strandat 23.313 kb on - strandat 23.313 kb on - strandat 23.398 kb on + strand, within Pf1N1B4_5266at 23.399 kb on - strand, within Pf1N1B4_5266at 23.533 kb on - strand, within Pf1N1B4_5266at 23.533 kb on - strand, within Pf1N1B4_5266at 23.686 kb on + strandat 23.687 kb on - strandat 23.693 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
remove
21,589 + +0.8
21,724 + Pf1N1B4_5262 0.36 -0.1
21,725 - Pf1N1B4_5262 0.37 -1.2
21,904 + Pf1N1B4_5262 0.73 -0.8
21,904 + Pf1N1B4_5262 0.73 +0.8
21,904 + Pf1N1B4_5262 0.73 +0.8
21,905 - Pf1N1B4_5262 0.73 +0.8
21,905 - Pf1N1B4_5262 0.73 +0.8
21,905 - Pf1N1B4_5262 0.73 -1.0
21,905 - Pf1N1B4_5262 0.73 -2.8
21,941 + Pf1N1B4_5262 0.81 -0.4
21,941 + Pf1N1B4_5262 0.81 +0.7
21,943 + Pf1N1B4_5262 0.81 -2.2
21,944 - Pf1N1B4_5262 0.81 -0.2
21,944 - Pf1N1B4_5262 0.81 +0.8
21,961 + Pf1N1B4_5262 0.85 -1.8
22,078 - -0.5
22,104 + -0.2
22,105 - -0.0
22,105 - -0.6
22,159 + -1.6
22,159 + -1.8
22,160 - +0.4
22,162 + +0.8
22,162 + -0.1
22,162 + -0.6
22,162 + +1.8
22,162 + -0.2
22,527 + -2.3
22,528 - +0.8
22,541 + -3.3
22,541 + +0.8
22,542 - -0.2
22,542 - +3.6
22,542 - -0.2
22,542 - -0.3
22,604 + Pf1N1B4_5263 0.39 +0.8
22,604 + Pf1N1B4_5263 0.39 -1.5
22,605 - Pf1N1B4_5263 0.40 -2.0
22,605 - Pf1N1B4_5263 0.40 -1.2
22,605 - Pf1N1B4_5263 0.40 -0.6
22,605 - Pf1N1B4_5263 0.40 -1.0
22,605 - Pf1N1B4_5263 0.40 -2.2
22,605 - Pf1N1B4_5263 0.40 -2.4
22,605 - Pf1N1B4_5263 0.40 -0.8
22,605 - Pf1N1B4_5263 0.40 +0.8
22,605 - Pf1N1B4_5263 0.40 +0.8
22,605 - Pf1N1B4_5263 0.40 +0.8
22,670 + Pf1N1B4_5263 0.73 -1.6
22,671 - Pf1N1B4_5263 0.73 -2.0
22,671 - +0.8
22,671 - -1.5
22,671 - Pf1N1B4_5263 0.73 -1.8
22,718 - +0.0
22,718 - -0.3
22,797 + Pf1N1B4_5264 0.47 -1.0
22,798 - -2.2
22,878 + -2.8
22,879 - -1.8
22,879 - +0.8
22,905 - -2.8
22,906 + -2.2
22,907 - -1.0
22,907 - -2.3
22,938 - -0.4
22,958 - +1.1
22,974 - -0.5
23,042 - -0.8
23,042 - -0.3
23,042 - +0.8
23,042 - +0.8
23,042 - -2.3
23,077 - +0.8
23,077 - +2.7
23,211 - Pf1N1B4_5265 0.33 -3.7
23,211 - Pf1N1B4_5265 0.33 -3.7
23,211 - Pf1N1B4_5265 0.33 +0.1
23,251 + Pf1N1B4_5265 0.64 +0.8
23,252 - Pf1N1B4_5265 0.65 +0.8
23,252 - Pf1N1B4_5265 0.65 +0.2
23,252 - Pf1N1B4_5265 0.65 +0.2
23,252 - Pf1N1B4_5265 0.65 -1.2
23,252 - Pf1N1B4_5265 0.65 -0.6
23,312 + -1.2
23,312 + +0.8
23,313 - +0.2
23,313 - +0.8
23,398 + Pf1N1B4_5266 0.25 -3.3
23,399 - Pf1N1B4_5266 0.25 +0.5
23,533 - Pf1N1B4_5266 0.67 +0.8
23,533 - Pf1N1B4_5266 0.67 -0.6
23,686 + +0.8
23,687 - +0.8
23,693 - -0.6

Or see this region's nucleotide sequence