Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5122 and Pf1N1B4_5123 are separated by 420 nucleotides Pf1N1B4_5123 and Pf1N1B4_5124 are separated by 249 nucleotides
Pf1N1B4_5122 - S-layer protein, at 5,688,151 to 5,691,807
_5122
Pf1N1B4_5123 - T1SS secreted agglutinin RTX, at 5,692,228 to 5,700,051
_5123
Pf1N1B4_5124 - two component transcriptional regulator, LuxR family, at 5,700,301 to 5,700,918
_5124
Position (kb) 5692
5693
5694
5695
5696
5697
5698
5699
5700
5701 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5691.486 kb on + strand at 5691.543 kb on + strand at 5691.661 kb on + strand at 5691.661 kb on + strand at 5691.661 kb on + strand at 5691.662 kb on - strand at 5691.670 kb on + strand at 5691.670 kb on + strand at 5691.755 kb on + strand at 5691.813 kb on + strand at 5691.813 kb on + strand at 5692.106 kb on + strand at 5692.169 kb on + strand at 5692.489 kb on - strand at 5692.489 kb on - strand at 5692.589 kb on - strand at 5693.111 kb on - strand, within Pf1N1B4_5123 at 5693.252 kb on - strand, within Pf1N1B4_5123 at 5693.320 kb on + strand, within Pf1N1B4_5123 at 5693.320 kb on + strand, within Pf1N1B4_5123 at 5693.492 kb on - strand, within Pf1N1B4_5123 at 5693.834 kb on - strand, within Pf1N1B4_5123 at 5698.120 kb on + strand at 5698.575 kb on + strand, within Pf1N1B4_5123 at 5698.638 kb on + strand, within Pf1N1B4_5123 at 5698.639 kb on - strand, within Pf1N1B4_5123 at 5698.642 kb on + strand, within Pf1N1B4_5123 at 5698.643 kb on - strand, within Pf1N1B4_5123 at 5698.643 kb on - strand, within Pf1N1B4_5123 at 5698.643 kb on - strand, within Pf1N1B4_5123 at 5698.721 kb on - strand, within Pf1N1B4_5123 at 5698.921 kb on - strand, within Pf1N1B4_5123 at 5699.278 kb on - strand at 5699.362 kb on + strand at 5699.386 kb on + strand at 5699.386 kb on + strand at 5699.935 kb on + strand at 5699.935 kb on + strand at 5700.368 kb on + strand, within Pf1N1B4_5124 at 5700.410 kb on + strand at 5700.476 kb on + strand, within Pf1N1B4_5124 at 5700.476 kb on + strand, within Pf1N1B4_5124 at 5700.477 kb on - strand, within Pf1N1B4_5124 at 5700.477 kb on - strand, within Pf1N1B4_5124 at 5700.526 kb on + strand, within Pf1N1B4_5124 at 5700.574 kb on + strand, within Pf1N1B4_5124 at 5700.575 kb on - strand at 5700.576 kb on + strand, within Pf1N1B4_5124 at 5700.577 kb on - strand at 5700.890 kb on + strand at 5700.891 kb on - strand at 5700.994 kb on + strand at 5700.995 kb on - strand at 5701.045 kb on + strand at 5701.046 kb on - strand at 5701.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,691,486 + -1.4 5,691,543 + +0.4 5,691,661 + -1.0 5,691,661 + -0.9 5,691,661 + +0.4 5,691,662 - -0.6 5,691,670 + +0.8 5,691,670 + -0.4 5,691,755 + +0.4 5,691,813 + +0.0 5,691,813 + -0.9 5,692,106 + -0.8 5,692,169 + -0.8 5,692,489 - -0.6 5,692,489 - -1.6 5,692,589 - +0.4 5,693,111 - Pf1N1B4_5123 0.11 +0.4 5,693,252 - Pf1N1B4_5123 0.13 +0.9 5,693,320 + Pf1N1B4_5123 0.14 +0.4 5,693,320 + Pf1N1B4_5123 0.14 -0.1 5,693,492 - Pf1N1B4_5123 0.16 -0.2 5,693,834 - Pf1N1B4_5123 0.21 +0.4 5,698,120 + +0.4 5,698,575 + Pf1N1B4_5123 0.81 +0.4 5,698,638 + Pf1N1B4_5123 0.82 +1.1 5,698,639 - Pf1N1B4_5123 0.82 +0.2 5,698,642 + Pf1N1B4_5123 0.82 -0.6 5,698,643 - Pf1N1B4_5123 0.82 -4.4 5,698,643 - Pf1N1B4_5123 0.82 +3.8 5,698,643 - Pf1N1B4_5123 0.82 -2.4 5,698,721 - Pf1N1B4_5123 0.83 +1.0 5,698,921 - Pf1N1B4_5123 0.86 -0.0 5,699,278 - -0.4 5,699,362 + -0.7 5,699,386 + -0.0 5,699,386 + +0.4 5,699,935 + -1.8 5,699,935 + +0.4 5,700,368 + Pf1N1B4_5124 0.11 -0.9 5,700,410 + -0.3 5,700,476 + Pf1N1B4_5124 0.28 -0.6 5,700,476 + Pf1N1B4_5124 0.28 -2.8 5,700,477 - Pf1N1B4_5124 0.28 -0.2 5,700,477 - Pf1N1B4_5124 0.28 +1.1 5,700,526 + Pf1N1B4_5124 0.36 +0.4 5,700,574 + Pf1N1B4_5124 0.44 +0.4 5,700,575 - -3.2 5,700,576 + Pf1N1B4_5124 0.44 -0.4 5,700,577 - +0.4 5,700,890 + -1.4 5,700,891 - -2.2 5,700,994 + -0.9 5,700,995 - +0.6 5,701,045 + -2.2 5,701,046 - +0.4 5,701,048 - -3.6
Or see this region's nucleotide sequence