Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5022 and Pf1N1B4_5023 overlap by 11 nucleotides Pf1N1B4_5023 and Pf1N1B4_5024 are separated by 60 nucleotides Pf1N1B4_5024 and Pf1N1B4_5025 overlap by 37 nucleotides Pf1N1B4_5025 and Pf1N1B4_5026 are separated by 53 nucleotides
Pf1N1B4_5022 - hypothetical protein, at 5,570,004 to 5,570,714
_5022
Pf1N1B4_5023 - Alkylated DNA repair protein AlkB, at 5,570,704 to 5,571,384
_5023
Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545
_5024
Pf1N1B4_5025 - Probable transcription regulator, at 5,572,509 to 5,572,997
_5025
Pf1N1B4_5026 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,573,051 to 5,574,034
_5026
Position (kb) 5571
5572
5573 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5570.800 kb on + strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.907 kb on + strand, within Pf1N1B4_5023 at 5570.908 kb on - strand at 5570.908 kb on - strand, within Pf1N1B4_5023 at 5570.974 kb on + strand, within Pf1N1B4_5023 at 5571.108 kb on + strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.418 kb on - strand at 5571.418 kb on - strand at 5571.437 kb on + strand at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.589 kb on + strand, within Pf1N1B4_5024 at 5571.590 kb on - strand at 5571.590 kb on - strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.946 kb on + strand, within Pf1N1B4_5024 at 5571.947 kb on - strand, within Pf1N1B4_5024 at 5571.979 kb on + strand, within Pf1N1B4_5024 at 5571.979 kb on + strand, within Pf1N1B4_5024 at 5571.980 kb on - strand, within Pf1N1B4_5024 at 5572.280 kb on + strand, within Pf1N1B4_5024 at 5572.351 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.362 kb on - strand, within Pf1N1B4_5024 at 5572.582 kb on + strand, within Pf1N1B4_5025 at 5572.583 kb on - strand, within Pf1N1B4_5025 at 5572.637 kb on - strand, within Pf1N1B4_5025 at 5572.637 kb on - strand, within Pf1N1B4_5025 at 5572.705 kb on + strand, within Pf1N1B4_5025 at 5572.804 kb on + strand at 5572.805 kb on - strand, within Pf1N1B4_5025 at 5572.805 kb on - strand, within Pf1N1B4_5025 at 5572.814 kb on - strand, within Pf1N1B4_5025 at 5572.822 kb on + strand, within Pf1N1B4_5025 at 5572.892 kb on + strand, within Pf1N1B4_5025 at 5572.893 kb on - strand, within Pf1N1B4_5025 at 5572.910 kb on + strand, within Pf1N1B4_5025 at 5572.989 kb on + strand at 5572.989 kb on + strand at 5572.989 kb on + strand at 5572.990 kb on - strand at 5572.990 kb on - strand at 5572.990 kb on - strand at 5573.018 kb on + strand at 5573.492 kb on - strand, within Pf1N1B4_5026
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,570,800 + Pf1N1B4_5023 0.14 -1.9 5,570,801 - Pf1N1B4_5023 0.14 -0.6 5,570,801 - Pf1N1B4_5023 0.14 -1.6 5,570,801 - Pf1N1B4_5023 0.14 -2.2 5,570,907 + Pf1N1B4_5023 0.30 +0.1 5,570,908 - +0.4 5,570,908 - Pf1N1B4_5023 0.30 -0.4 5,570,974 + Pf1N1B4_5023 0.40 -1.3 5,571,108 + Pf1N1B4_5023 0.59 +0.4 5,571,109 - Pf1N1B4_5023 0.59 -1.3 5,571,109 - +0.4 5,571,109 - Pf1N1B4_5023 0.59 +0.4 5,571,109 - Pf1N1B4_5023 0.59 +0.4 5,571,418 - -0.2 5,571,418 - -1.9 5,571,437 + +0.4 5,571,566 - Pf1N1B4_5024 0.11 +0.2 5,571,566 - Pf1N1B4_5024 0.11 -2.2 5,571,566 - Pf1N1B4_5024 0.11 +1.1 5,571,566 - Pf1N1B4_5024 0.11 -1.5 5,571,589 + Pf1N1B4_5024 0.13 -1.2 5,571,590 - +0.4 5,571,590 - Pf1N1B4_5024 0.13 +0.4 5,571,946 + Pf1N1B4_5024 0.46 -1.6 5,571,946 + Pf1N1B4_5024 0.46 -2.3 5,571,946 + Pf1N1B4_5024 0.46 -1.6 5,571,946 + Pf1N1B4_5024 0.46 -1.4 5,571,946 + Pf1N1B4_5024 0.46 -3.9 5,571,947 - Pf1N1B4_5024 0.46 -0.5 5,571,979 + Pf1N1B4_5024 0.49 -2.2 5,571,979 + Pf1N1B4_5024 0.49 +0.4 5,571,980 - Pf1N1B4_5024 0.49 -1.5 5,572,280 + Pf1N1B4_5024 0.76 -1.6 5,572,351 + Pf1N1B4_5024 0.82 -1.9 5,572,361 + Pf1N1B4_5024 0.83 +0.2 5,572,361 + Pf1N1B4_5024 0.83 -2.0 5,572,361 + Pf1N1B4_5024 0.83 -0.2 5,572,362 - Pf1N1B4_5024 0.83 +0.1 5,572,582 + Pf1N1B4_5025 0.15 +0.4 5,572,583 - Pf1N1B4_5025 0.15 -0.9 5,572,637 - Pf1N1B4_5025 0.26 -0.6 5,572,637 - Pf1N1B4_5025 0.26 +0.1 5,572,705 + Pf1N1B4_5025 0.40 +0.0 5,572,804 + -2.2 5,572,805 - Pf1N1B4_5025 0.61 -1.9 5,572,805 - Pf1N1B4_5025 0.61 -0.5 5,572,814 - Pf1N1B4_5025 0.62 -3.3 5,572,822 + Pf1N1B4_5025 0.64 -1.8 5,572,892 + Pf1N1B4_5025 0.78 +0.5 5,572,893 - Pf1N1B4_5025 0.79 -0.2 5,572,910 + Pf1N1B4_5025 0.82 -3.1 5,572,989 + -0.4 5,572,989 + -3.1 5,572,989 + -1.8 5,572,990 - -0.5 5,572,990 - -0.3 5,572,990 - +0.4 5,573,018 + +0.4 5,573,492 - Pf1N1B4_5026 0.45 +0.4
Or see this region's nucleotide sequence