Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4910 and Pf1N1B4_4911 are separated by 95 nucleotides Pf1N1B4_4911 and Pf1N1B4_4912 are separated by 45 nucleotides Pf1N1B4_4912 and Pf1N1B4_4913 are separated by 448 nucleotides
Pf1N1B4_4910 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 5,440,060 to 5,441,352
_4910
Pf1N1B4_4911 - FIG00953249: hypothetical protein, at 5,441,448 to 5,442,104
_4911
Pf1N1B4_4912 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,442,150 to 5,442,638
_4912
Pf1N1B4_4913 - hypothetical protein, at 5,443,087 to 5,443,866
_4913
Position (kb)
5442
5443 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5441.402 kb on - strand at 5441.402 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5441.441 kb on - strand at 5442.045 kb on + strand at 5442.125 kb on - strand at 5442.125 kb on - strand at 5442.161 kb on - strand at 5442.164 kb on - strand at 5442.303 kb on + strand, within Pf1N1B4_4912 at 5442.306 kb on - strand, within Pf1N1B4_4912 at 5442.316 kb on - strand at 5442.362 kb on - strand, within Pf1N1B4_4912 at 5442.443 kb on - strand, within Pf1N1B4_4912 at 5442.565 kb on - strand, within Pf1N1B4_4912 at 5442.963 kb on + strand at 5443.008 kb on + strand at 5443.009 kb on - strand at 5443.009 kb on - strand at 5443.009 kb on - strand at 5443.205 kb on - strand, within Pf1N1B4_4913 at 5443.419 kb on + strand, within Pf1N1B4_4913 at 5443.552 kb on + strand, within Pf1N1B4_4913
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,441,402 - -0.5 5,441,402 - -1.6 5,441,441 - -0.9 5,441,441 - -0.3 5,441,441 - -1.0 5,442,045 + +0.6 5,442,125 - -0.4 5,442,125 - -1.5 5,442,161 - -3.3 5,442,164 - +0.4 5,442,303 + Pf1N1B4_4912 0.31 +0.9 5,442,306 - Pf1N1B4_4912 0.32 -0.2 5,442,316 - +0.4 5,442,362 - Pf1N1B4_4912 0.43 +1.6 5,442,443 - Pf1N1B4_4912 0.60 +0.4 5,442,565 - Pf1N1B4_4912 0.85 +0.4 5,442,963 + +0.3 5,443,008 + -0.1 5,443,009 - +0.4 5,443,009 - -2.4 5,443,009 - +1.2 5,443,205 - Pf1N1B4_4913 0.15 -2.7 5,443,419 + Pf1N1B4_4913 0.43 +0.4 5,443,552 + Pf1N1B4_4913 0.60 +1.7
Or see this region's nucleotide sequence