Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_488

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_487 and Pf1N1B4_488 are separated by 263 nucleotidesPf1N1B4_488 and Pf1N1B4_489 are separated by 91 nucleotides Pf1N1B4_487 - Sialic acid transporter (permease) NanT, at 578,466 to 579,761 _487 Pf1N1B4_488 - Cyn operon transcriptional activator, at 580,025 to 580,972 _488 Pf1N1B4_489 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 581,064 to 581,867 _489 Position (kb) 580 581Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 579.030 kb on - strand, within Pf1N1B4_487at 579.030 kb on - strand, within Pf1N1B4_487at 579.032 kb on + strand, within Pf1N1B4_487at 579.032 kb on + strand, within Pf1N1B4_487at 579.032 kb on + strand, within Pf1N1B4_487at 579.033 kb on - strand, within Pf1N1B4_487at 579.033 kb on - strandat 579.033 kb on - strand, within Pf1N1B4_487at 579.033 kb on - strand, within Pf1N1B4_487at 579.238 kb on + strand, within Pf1N1B4_487at 579.239 kb on - strand, within Pf1N1B4_487at 579.406 kb on + strand, within Pf1N1B4_487at 579.407 kb on - strandat 579.407 kb on - strand, within Pf1N1B4_487at 579.498 kb on + strand, within Pf1N1B4_487at 579.499 kb on - strand, within Pf1N1B4_487at 579.807 kb on - strandat 579.913 kb on + strandat 579.914 kb on - strandat 579.920 kb on - strandat 579.957 kb on - strandat 579.963 kb on + strandat 579.963 kb on + strandat 579.963 kb on + strandat 579.964 kb on - strandat 579.964 kb on - strandat 579.964 kb on - strandat 579.964 kb on - strandat 580.118 kb on - strandat 580.224 kb on - strand, within Pf1N1B4_488at 580.509 kb on - strand, within Pf1N1B4_488at 580.544 kb on + strand, within Pf1N1B4_488at 580.545 kb on - strand, within Pf1N1B4_488at 580.545 kb on - strand, within Pf1N1B4_488at 580.545 kb on - strand, within Pf1N1B4_488at 580.545 kb on - strand, within Pf1N1B4_488at 580.545 kb on - strand, within Pf1N1B4_488at 580.556 kb on + strand, within Pf1N1B4_488at 580.629 kb on + strand, within Pf1N1B4_488at 580.629 kb on + strand, within Pf1N1B4_488at 580.629 kb on + strand, within Pf1N1B4_488at 580.630 kb on - strand, within Pf1N1B4_488at 580.630 kb on - strand, within Pf1N1B4_488at 580.630 kb on - strand, within Pf1N1B4_488at 580.970 kb on + strandat 580.971 kb on - strandat 580.971 kb on - strandat 580.978 kb on - strandat 580.978 kb on - strandat 580.978 kb on - strandat 580.978 kb on - strandat 581.145 kb on + strand, within Pf1N1B4_489at 581.146 kb on - strand, within Pf1N1B4_489at 581.355 kb on + strand, within Pf1N1B4_489at 581.518 kb on - strand, within Pf1N1B4_489at 581.518 kb on - strand, within Pf1N1B4_489at 581.518 kb on - strand, within Pf1N1B4_489at 581.520 kb on - strand, within Pf1N1B4_489at 581.530 kb on + strand, within Pf1N1B4_489at 581.530 kb on + strandat 581.530 kb on + strand, within Pf1N1B4_489at 581.530 kb on + strand, within Pf1N1B4_489at 581.531 kb on - strandat 581.531 kb on - strand, within Pf1N1B4_489at 581.531 kb on - strand, within Pf1N1B4_489at 581.531 kb on - strand, within Pf1N1B4_489at 581.531 kb on - strandat 581.531 kb on - strand, within Pf1N1B4_489at 581.785 kb on + strand, within Pf1N1B4_489at 581.786 kb on - strand, within Pf1N1B4_489at 581.894 kb on - strandat 581.966 kb on + strandat 581.966 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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579,030 - Pf1N1B4_487 0.44 -0.3
579,030 - Pf1N1B4_487 0.44 -0.1
579,032 + Pf1N1B4_487 0.44 +0.2
579,032 + Pf1N1B4_487 0.44 +0.0
579,032 + Pf1N1B4_487 0.44 -0.1
579,033 - Pf1N1B4_487 0.44 +1.0
579,033 - -0.8
579,033 - Pf1N1B4_487 0.44 -0.7
579,033 - Pf1N1B4_487 0.44 -0.3
579,238 + Pf1N1B4_487 0.60 -0.8
579,239 - Pf1N1B4_487 0.60 +1.3
579,406 + Pf1N1B4_487 0.73 +0.7
579,407 - +0.7
579,407 - Pf1N1B4_487 0.73 -3.4
579,498 + Pf1N1B4_487 0.80 -0.8
579,499 - Pf1N1B4_487 0.80 -0.7
579,807 - +1.3
579,913 + -1.3
579,914 - -2.6
579,920 - -1.8
579,957 - -0.6
579,963 + +0.7
579,963 + +0.6
579,963 + -0.4
579,964 - +0.7
579,964 - +0.7
579,964 - +0.4
579,964 - +0.7
580,118 - -1.2
580,224 - Pf1N1B4_488 0.21 +1.6
580,509 - Pf1N1B4_488 0.51 +2.1
580,544 + Pf1N1B4_488 0.55 -1.8
580,545 - Pf1N1B4_488 0.55 +0.7
580,545 - Pf1N1B4_488 0.55 -2.3
580,545 - Pf1N1B4_488 0.55 -0.8
580,545 - Pf1N1B4_488 0.55 +1.6
580,545 - Pf1N1B4_488 0.55 -2.4
580,556 + Pf1N1B4_488 0.56 +0.7
580,629 + Pf1N1B4_488 0.64 -0.2
580,629 + Pf1N1B4_488 0.64 -1.1
580,629 + Pf1N1B4_488 0.64 +0.7
580,630 - Pf1N1B4_488 0.64 -1.6
580,630 - Pf1N1B4_488 0.64 +0.7
580,630 - Pf1N1B4_488 0.64 -0.3
580,970 + +0.7
580,971 - -0.8
580,971 - -0.5
580,978 - -2.7
580,978 - -1.8
580,978 - +0.7
580,978 - +1.1
581,145 + Pf1N1B4_489 0.10 -1.2
581,146 - Pf1N1B4_489 0.10 +0.7
581,355 + Pf1N1B4_489 0.36 +0.7
581,518 - Pf1N1B4_489 0.56 -0.6
581,518 - Pf1N1B4_489 0.56 -1.6
581,518 - Pf1N1B4_489 0.56 -0.9
581,520 - Pf1N1B4_489 0.57 +0.7
581,530 + Pf1N1B4_489 0.58 -0.5
581,530 + +0.1
581,530 + Pf1N1B4_489 0.58 -0.8
581,530 + Pf1N1B4_489 0.58 +0.7
581,531 - +1.7
581,531 - Pf1N1B4_489 0.58 +0.7
581,531 - Pf1N1B4_489 0.58 +0.7
581,531 - Pf1N1B4_489 0.58 -2.1
581,531 - -1.3
581,531 - Pf1N1B4_489 0.58 -0.3
581,785 + Pf1N1B4_489 0.90 +0.7
581,786 - Pf1N1B4_489 0.90 +0.7
581,894 - -0.7
581,966 + -1.8
581,966 + +0.2

Or see this region's nucleotide sequence