Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4867 and Pf1N1B4_4868 are separated by 2 nucleotides Pf1N1B4_4868 and Pf1N1B4_4869 are separated by 83 nucleotides
Pf1N1B4_4867 - FIG00464907: hypothetical protein, at 5,384,694 to 5,385,713
_4867
Pf1N1B4_4868 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 5,385,716 to 5,388,721
_4868
Pf1N1B4_4869 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,388,805 to 5,391,045
_4869
Position (kb) 5385
5386
5387
5388
5389 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5385.195 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.258 kb on - strand, within Pf1N1B4_4867 at 5385.258 kb on - strand, within Pf1N1B4_4867 at 5385.381 kb on + strand, within Pf1N1B4_4867 at 5385.381 kb on + strand, within Pf1N1B4_4867 at 5385.382 kb on - strand, within Pf1N1B4_4867 at 5385.485 kb on - strand, within Pf1N1B4_4867 at 5385.498 kb on + strand, within Pf1N1B4_4867 at 5385.690 kb on + strand at 5385.690 kb on + strand at 5385.691 kb on - strand at 5385.691 kb on - strand at 5385.691 kb on - strand at 5385.691 kb on - strand at 5385.724 kb on + strand at 5386.109 kb on - strand, within Pf1N1B4_4868 at 5386.240 kb on - strand, within Pf1N1B4_4868 at 5386.240 kb on - strand, within Pf1N1B4_4868 at 5386.475 kb on + strand, within Pf1N1B4_4868 at 5386.475 kb on + strand at 5386.476 kb on - strand at 5386.476 kb on - strand, within Pf1N1B4_4868 at 5386.488 kb on - strand, within Pf1N1B4_4868 at 5386.632 kb on + strand, within Pf1N1B4_4868 at 5386.633 kb on - strand, within Pf1N1B4_4868 at 5387.019 kb on - strand, within Pf1N1B4_4868 at 5387.212 kb on - strand, within Pf1N1B4_4868 at 5387.343 kb on - strand, within Pf1N1B4_4868 at 5387.345 kb on - strand at 5387.420 kb on + strand, within Pf1N1B4_4868 at 5387.642 kb on - strand, within Pf1N1B4_4868 at 5387.701 kb on + strand at 5387.701 kb on + strand, within Pf1N1B4_4868 at 5387.701 kb on + strand, within Pf1N1B4_4868 at 5387.795 kb on + strand, within Pf1N1B4_4868 at 5387.834 kb on + strand, within Pf1N1B4_4868 at 5387.851 kb on + strand, within Pf1N1B4_4868 at 5387.854 kb on + strand, within Pf1N1B4_4868 at 5387.854 kb on + strand, within Pf1N1B4_4868 at 5387.855 kb on - strand at 5387.855 kb on - strand at 5388.139 kb on + strand at 5388.139 kb on + strand at 5388.233 kb on + strand at 5388.233 kb on + strand, within Pf1N1B4_4868 at 5388.234 kb on - strand, within Pf1N1B4_4868 at 5388.234 kb on - strand, within Pf1N1B4_4868 at 5388.615 kb on + strand at 5388.688 kb on - strand at 5388.993 kb on + strand at 5389.076 kb on - strand, within Pf1N1B4_4869 at 5389.181 kb on + strand, within Pf1N1B4_4869 at 5389.182 kb on - strand, within Pf1N1B4_4869 at 5389.332 kb on - strand, within Pf1N1B4_4869
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,385,195 + Pf1N1B4_4867 0.49 +0.2 5,385,257 + Pf1N1B4_4867 0.55 -1.2 5,385,257 + Pf1N1B4_4867 0.55 -1.0 5,385,257 + +0.4 5,385,257 + Pf1N1B4_4867 0.55 -0.9 5,385,258 - Pf1N1B4_4867 0.55 -1.6 5,385,258 - Pf1N1B4_4867 0.55 +0.1 5,385,381 + Pf1N1B4_4867 0.67 +0.4 5,385,381 + Pf1N1B4_4867 0.67 -0.4 5,385,382 - Pf1N1B4_4867 0.67 -2.3 5,385,485 - Pf1N1B4_4867 0.78 -0.1 5,385,498 + Pf1N1B4_4867 0.79 +0.4 5,385,690 + +0.4 5,385,690 + +0.2 5,385,691 - -3.5 5,385,691 - -0.8 5,385,691 - -0.8 5,385,691 - -0.4 5,385,724 + -1.8 5,386,109 - Pf1N1B4_4868 0.13 -0.6 5,386,240 - Pf1N1B4_4868 0.17 -3.4 5,386,240 - Pf1N1B4_4868 0.17 -0.9 5,386,475 + Pf1N1B4_4868 0.25 -1.4 5,386,475 + -0.8 5,386,476 - -0.9 5,386,476 - Pf1N1B4_4868 0.25 -2.6 5,386,488 - Pf1N1B4_4868 0.26 +0.4 5,386,632 + Pf1N1B4_4868 0.30 +0.4 5,386,633 - Pf1N1B4_4868 0.31 -1.1 5,387,019 - Pf1N1B4_4868 0.43 -0.2 5,387,212 - Pf1N1B4_4868 0.50 -1.9 5,387,343 - Pf1N1B4_4868 0.54 -2.0 5,387,345 - +2.1 5,387,420 + Pf1N1B4_4868 0.57 -2.4 5,387,642 - Pf1N1B4_4868 0.64 -4.3 5,387,701 + +0.1 5,387,701 + Pf1N1B4_4868 0.66 +0.4 5,387,701 + Pf1N1B4_4868 0.66 +0.4 5,387,795 + Pf1N1B4_4868 0.69 +2.3 5,387,834 + Pf1N1B4_4868 0.70 -0.4 5,387,851 + Pf1N1B4_4868 0.71 -2.6 5,387,854 + Pf1N1B4_4868 0.71 -4.4 5,387,854 + Pf1N1B4_4868 0.71 -1.1 5,387,855 - -0.6 5,387,855 - +0.4 5,388,139 + +0.4 5,388,139 + -1.1 5,388,233 + +0.4 5,388,233 + Pf1N1B4_4868 0.84 -2.1 5,388,234 - Pf1N1B4_4868 0.84 -0.2 5,388,234 - Pf1N1B4_4868 0.84 -2.4 5,388,615 + -0.4 5,388,688 - +0.1 5,388,993 + +0.2 5,389,076 - Pf1N1B4_4869 0.12 -3.9 5,389,181 + Pf1N1B4_4869 0.17 +0.4 5,389,182 - Pf1N1B4_4869 0.17 -1.9 5,389,332 - Pf1N1B4_4869 0.24 +0.4
Or see this region's nucleotide sequence