Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4817 and Pf1N1B4_4818 are separated by 0 nucleotides Pf1N1B4_4818 and Pf1N1B4_4819 are separated by 80 nucleotides
Pf1N1B4_4817 - Fe2+-dicitrate sensor, membrane component, at 5,321,289 to 5,322,266
_4817
Pf1N1B4_4818 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,322,267 to 5,322,782
_4818
Pf1N1B4_4819 - Multidrug resistance protein B, at 5,322,863 to 5,324,107
_4819
Position (kb) 5322
5323 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5321.274 kb on + strand at 5321.323 kb on - strand at 5321.439 kb on + strand, within Pf1N1B4_4817 at 5321.490 kb on + strand, within Pf1N1B4_4817 at 5321.490 kb on + strand, within Pf1N1B4_4817 at 5321.490 kb on + strand, within Pf1N1B4_4817 at 5321.491 kb on - strand, within Pf1N1B4_4817 at 5321.491 kb on - strand, within Pf1N1B4_4817 at 5321.766 kb on + strand, within Pf1N1B4_4817 at 5321.767 kb on - strand at 5322.361 kb on + strand, within Pf1N1B4_4818 at 5322.361 kb on + strand, within Pf1N1B4_4818 at 5322.396 kb on - strand at 5322.396 kb on - strand, within Pf1N1B4_4818 at 5322.701 kb on - strand, within Pf1N1B4_4818 at 5322.701 kb on - strand, within Pf1N1B4_4818 at 5322.910 kb on - strand at 5322.945 kb on + strand at 5322.946 kb on - strand at 5322.946 kb on - strand at 5322.967 kb on - strand at 5322.967 kb on - strand at 5323.043 kb on - strand, within Pf1N1B4_4819 at 5323.066 kb on - strand at 5323.152 kb on + strand, within Pf1N1B4_4819 at 5323.219 kb on + strand, within Pf1N1B4_4819 at 5323.273 kb on - strand, within Pf1N1B4_4819 at 5323.371 kb on + strand, within Pf1N1B4_4819 at 5323.371 kb on + strand, within Pf1N1B4_4819 at 5323.372 kb on - strand, within Pf1N1B4_4819 at 5323.372 kb on - strand, within Pf1N1B4_4819 at 5323.466 kb on - strand, within Pf1N1B4_4819
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,321,274 + +0.4 5,321,323 - -0.5 5,321,439 + Pf1N1B4_4817 0.15 -0.6 5,321,490 + Pf1N1B4_4817 0.21 +0.6 5,321,490 + Pf1N1B4_4817 0.21 +0.4 5,321,490 + Pf1N1B4_4817 0.21 -0.6 5,321,491 - Pf1N1B4_4817 0.21 +1.0 5,321,491 - Pf1N1B4_4817 0.21 -2.6 5,321,766 + Pf1N1B4_4817 0.49 -1.1 5,321,767 - +0.4 5,322,361 + Pf1N1B4_4818 0.18 -2.6 5,322,361 + Pf1N1B4_4818 0.18 -2.6 5,322,396 - -0.5 5,322,396 - Pf1N1B4_4818 0.25 -3.9 5,322,701 - Pf1N1B4_4818 0.84 +0.4 5,322,701 - Pf1N1B4_4818 0.84 -0.8 5,322,910 - -0.2 5,322,945 + +0.0 5,322,946 - +0.4 5,322,946 - +0.2 5,322,967 - +0.7 5,322,967 - -0.4 5,323,043 - Pf1N1B4_4819 0.14 -0.8 5,323,066 - +0.4 5,323,152 + Pf1N1B4_4819 0.23 -1.9 5,323,219 + Pf1N1B4_4819 0.29 -1.1 5,323,273 - Pf1N1B4_4819 0.33 +1.2 5,323,371 + Pf1N1B4_4819 0.41 -0.0 5,323,371 + Pf1N1B4_4819 0.41 +0.4 5,323,372 - Pf1N1B4_4819 0.41 -0.4 5,323,372 - Pf1N1B4_4819 0.41 +0.1 5,323,466 - Pf1N1B4_4819 0.48 +1.4
Or see this region's nucleotide sequence