Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4735 and Pf1N1B4_4736 are separated by 51 nucleotides Pf1N1B4_4736 and Pf1N1B4_4737 are separated by 1 nucleotides Pf1N1B4_4737 and Pf1N1B4_4738 are separated by 698 nucleotides
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Pf1N1B4_4736 - Fap amyloid fibril minor component, at 5,234,006 to 5,234,578
_4736
Pf1N1B4_4737 - hypothetical protein, at 5,234,580 to 5,234,801
_4737
Pf1N1B4_4738 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 5,235,500 to 5,236,024
_4738
Position (kb)
5234
5235 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.349 kb on - strand, within Pf1N1B4_4735 at 5233.815 kb on + strand, within Pf1N1B4_4735 at 5233.847 kb on - strand at 5233.889 kb on + strand at 5233.890 kb on - strand at 5233.968 kb on - strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.982 kb on - strand at 5233.994 kb on + strand at 5234.021 kb on + strand at 5234.218 kb on + strand, within Pf1N1B4_4736 at 5234.219 kb on - strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.225 kb on - strand, within Pf1N1B4_4736 at 5234.228 kb on + strand, within Pf1N1B4_4736 at 5234.735 kb on + strand at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.234 kb on - strand at 5235.244 kb on + strand at 5235.353 kb on + strand at 5235.353 kb on + strand at 5235.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,233,035 - Pf1N1B4_4735 0.29 +0.4 5,233,035 - Pf1N1B4_4735 0.29 -0.8 5,233,349 - Pf1N1B4_4735 0.53 -2.2 5,233,815 + Pf1N1B4_4735 0.89 -1.6 5,233,847 - -0.6 5,233,889 + -0.6 5,233,890 - -1.3 5,233,968 - -0.1 5,233,971 + -2.8 5,233,971 + +0.4 5,233,971 + +1.6 5,233,982 - -0.7 5,233,994 + +0.1 5,234,021 + -0.8 5,234,218 + Pf1N1B4_4736 0.37 +0.4 5,234,219 - Pf1N1B4_4736 0.37 +0.4 5,234,224 + Pf1N1B4_4736 0.38 +0.8 5,234,224 + Pf1N1B4_4736 0.38 +0.4 5,234,225 - Pf1N1B4_4736 0.38 +0.4 5,234,228 + Pf1N1B4_4736 0.39 +1.9 5,234,735 + -0.6 5,235,233 + -0.8 5,235,233 + -0.3 5,235,233 + +2.4 5,235,234 - +0.4 5,235,244 + +0.4 5,235,353 + -2.2 5,235,353 + -1.6 5,235,439 + -3.8
Or see this region's nucleotide sequence