Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4710 and Pf1N1B4_4711 are separated by 68 nucleotides Pf1N1B4_4711 and Pf1N1B4_4712 are separated by 3 nucleotides Pf1N1B4_4712 and Pf1N1B4_4713 are separated by 55 nucleotides
Pf1N1B4_4710 - Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine, at 5,206,906 to 5,207,829
_4710
Pf1N1B4_4711 - hypothetical protein, at 5,207,898 to 5,209,475
_4711
Pf1N1B4_4712 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,209,479 to 5,209,952
_4712
Pf1N1B4_4713 - Xanthine permease, at 5,210,008 to 5,211,483
_4713
Position (kb) 5207
5208
5209
5210 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5207.024 kb on + strand, within Pf1N1B4_4710 at 5207.025 kb on - strand, within Pf1N1B4_4710 at 5207.025 kb on - strand, within Pf1N1B4_4710 at 5207.141 kb on + strand, within Pf1N1B4_4710 at 5207.240 kb on + strand, within Pf1N1B4_4710 at 5207.240 kb on + strand, within Pf1N1B4_4710 at 5207.240 kb on + strand, within Pf1N1B4_4710 at 5207.241 kb on - strand, within Pf1N1B4_4710 at 5207.242 kb on + strand at 5207.257 kb on + strand, within Pf1N1B4_4710 at 5207.437 kb on + strand at 5207.691 kb on - strand, within Pf1N1B4_4710 at 5207.735 kb on - strand at 5207.774 kb on + strand at 5207.775 kb on - strand at 5207.775 kb on - strand at 5207.775 kb on - strand at 5207.827 kb on + strand at 5207.946 kb on - strand at 5207.946 kb on - strand at 5207.952 kb on + strand at 5207.953 kb on - strand at 5207.953 kb on - strand at 5207.953 kb on - strand at 5207.953 kb on - strand at 5208.132 kb on + strand, within Pf1N1B4_4711 at 5208.132 kb on + strand, within Pf1N1B4_4711 at 5208.132 kb on + strand, within Pf1N1B4_4711 at 5208.133 kb on - strand, within Pf1N1B4_4711 at 5208.204 kb on + strand, within Pf1N1B4_4711 at 5208.204 kb on + strand, within Pf1N1B4_4711 at 5208.205 kb on - strand, within Pf1N1B4_4711 at 5208.205 kb on - strand, within Pf1N1B4_4711 at 5208.205 kb on - strand at 5208.307 kb on - strand, within Pf1N1B4_4711 at 5208.570 kb on + strand, within Pf1N1B4_4711 at 5208.580 kb on + strand, within Pf1N1B4_4711 at 5208.893 kb on + strand, within Pf1N1B4_4711 at 5208.894 kb on - strand, within Pf1N1B4_4711 at 5209.186 kb on - strand at 5209.281 kb on + strand, within Pf1N1B4_4711 at 5209.411 kb on + strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.412 kb on - strand at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.579 kb on + strand, within Pf1N1B4_4712 at 5209.580 kb on - strand, within Pf1N1B4_4712 at 5209.765 kb on + strand at 5210.021 kb on - strand at 5210.154 kb on + strand at 5210.155 kb on - strand at 5210.155 kb on - strand at 5210.236 kb on + strand, within Pf1N1B4_4713
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 5,207,024 + Pf1N1B4_4710 0.13 +0.5 5,207,025 - Pf1N1B4_4710 0.13 -0.0 5,207,025 - Pf1N1B4_4710 0.13 -1.6 5,207,141 + Pf1N1B4_4710 0.25 -0.6 5,207,240 + Pf1N1B4_4710 0.36 -0.8 5,207,240 + Pf1N1B4_4710 0.36 -1.1 5,207,240 + Pf1N1B4_4710 0.36 +1.0 5,207,241 - Pf1N1B4_4710 0.36 +1.2 5,207,242 + -0.6 5,207,257 + Pf1N1B4_4710 0.38 -0.9 5,207,437 + +0.4 5,207,691 - Pf1N1B4_4710 0.85 -0.2 5,207,735 - +0.4 5,207,774 + +1.0 5,207,775 - -1.9 5,207,775 - -1.2 5,207,775 - -1.4 5,207,827 + -0.3 5,207,946 - -1.2 5,207,946 - -0.6 5,207,952 + +0.2 5,207,953 - -1.9 5,207,953 - -0.6 5,207,953 - -1.2 5,207,953 - +0.4 5,208,132 + Pf1N1B4_4711 0.15 -1.7 5,208,132 + Pf1N1B4_4711 0.15 +2.3 5,208,132 + Pf1N1B4_4711 0.15 +0.2 5,208,133 - Pf1N1B4_4711 0.15 -0.5 5,208,204 + Pf1N1B4_4711 0.19 +0.7 5,208,204 + Pf1N1B4_4711 0.19 +0.4 5,208,205 - Pf1N1B4_4711 0.19 -0.8 5,208,205 - Pf1N1B4_4711 0.19 -0.7 5,208,205 - -2.4 5,208,307 - Pf1N1B4_4711 0.26 +1.3 5,208,570 + Pf1N1B4_4711 0.43 +0.3 5,208,580 + Pf1N1B4_4711 0.43 +0.2 5,208,893 + Pf1N1B4_4711 0.63 -0.2 5,208,894 - Pf1N1B4_4711 0.63 -1.9 5,209,186 - +0.4 5,209,281 + Pf1N1B4_4711 0.88 +0.4 5,209,411 + +0.4 5,209,412 - +0.4 5,209,412 - -1.5 5,209,412 - -1.0 5,209,412 - +0.4 5,209,412 - +0.6 5,209,412 - -0.7 5,209,412 - +0.4 5,209,579 + Pf1N1B4_4712 0.21 +1.0 5,209,579 + Pf1N1B4_4712 0.21 +0.4 5,209,579 + Pf1N1B4_4712 0.21 +0.4 5,209,580 - Pf1N1B4_4712 0.21 +0.4 5,209,765 + -1.2 5,210,021 - -1.6 5,210,154 + -0.7 5,210,155 - +1.4 5,210,155 - -0.3 5,210,236 + Pf1N1B4_4713 0.15 -0.8
Or see this region's nucleotide sequence