Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4668

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4667 and Pf1N1B4_4668 overlap by 4 nucleotidesPf1N1B4_4668 and Pf1N1B4_4669 overlap by 10 nucleotides Pf1N1B4_4667 - Acriflavin resistance plasma membrane protein, at 5,146,584 to 5,148,125 _4667 Pf1N1B4_4668 - Heavy metal RND efflux outer membrane protein, CzcC family, at 5,148,122 to 5,148,688 _4668 Pf1N1B4_4669 - Heavy metal RND efflux outer membrane protein, CzcC family, at 5,148,679 to 5,149,602 _4669 Position (kb) 5148 5149Strain fitness (log2 ratio) -3 -2 -1 0 1at 5147.248 kb on - strand, within Pf1N1B4_4667at 5147.619 kb on + strandat 5147.620 kb on - strand, within Pf1N1B4_4667at 5147.620 kb on - strand, within Pf1N1B4_4667at 5147.715 kb on + strand, within Pf1N1B4_4667at 5147.716 kb on - strand, within Pf1N1B4_4667at 5147.716 kb on - strand, within Pf1N1B4_4667at 5148.025 kb on - strandat 5148.239 kb on + strand, within Pf1N1B4_4668at 5148.272 kb on + strand, within Pf1N1B4_4668at 5148.273 kb on - strand, within Pf1N1B4_4668at 5148.273 kb on - strand, within Pf1N1B4_4668at 5148.273 kb on - strand, within Pf1N1B4_4668at 5148.345 kb on - strand, within Pf1N1B4_4668at 5148.570 kb on - strand, within Pf1N1B4_4668at 5148.715 kb on + strandat 5148.716 kb on - strandat 5148.716 kb on - strandat 5148.929 kb on + strand, within Pf1N1B4_4669at 5149.105 kb on + strand, within Pf1N1B4_4669at 5149.143 kb on + strand, within Pf1N1B4_4669at 5149.287 kb on + strandat 5149.334 kb on - strand, within Pf1N1B4_4669at 5149.334 kb on - strand, within Pf1N1B4_4669

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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5,147,248 - Pf1N1B4_4667 0.43 +0.3
5,147,619 + +0.4
5,147,620 - Pf1N1B4_4667 0.67 -0.7
5,147,620 - Pf1N1B4_4667 0.67 +0.4
5,147,715 + Pf1N1B4_4667 0.73 -1.0
5,147,716 - Pf1N1B4_4667 0.73 -2.4
5,147,716 - Pf1N1B4_4667 0.73 +0.2
5,148,025 - -1.2
5,148,239 + Pf1N1B4_4668 0.21 +0.4
5,148,272 + Pf1N1B4_4668 0.26 -2.9
5,148,273 - Pf1N1B4_4668 0.27 -2.6
5,148,273 - Pf1N1B4_4668 0.27 +0.4
5,148,273 - Pf1N1B4_4668 0.27 -2.7
5,148,345 - Pf1N1B4_4668 0.39 +0.7
5,148,570 - Pf1N1B4_4668 0.79 -1.2
5,148,715 + -1.9
5,148,716 - -0.3
5,148,716 - -0.4
5,148,929 + Pf1N1B4_4669 0.27 -0.2
5,149,105 + Pf1N1B4_4669 0.46 -1.9
5,149,143 + Pf1N1B4_4669 0.50 -2.1
5,149,287 + +0.4
5,149,334 - Pf1N1B4_4669 0.71 +0.4
5,149,334 - Pf1N1B4_4669 0.71 -1.5

Or see this region's nucleotide sequence