Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4506

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4505 and Pf1N1B4_4506 are separated by 238 nucleotidesPf1N1B4_4506 and Pf1N1B4_4507 overlap by 17 nucleotides Pf1N1B4_4505 - Coenzyme PQQ synthesis protein E, at 4,966,850 to 4,968,487 _4505 Pf1N1B4_4506 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 4,968,726 to 4,969,889 _4506 Pf1N1B4_4507 - Sensory box histidine kinase/response regulator, at 4,969,873 to 4,971,558 _4507 Position (kb) 4968 4969 4970Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4967.732 kb on - strand, within Pf1N1B4_4505at 4968.066 kb on - strand, within Pf1N1B4_4505at 4968.695 kb on - strandat 4968.753 kb on - strandat 4968.810 kb on + strandat 4968.930 kb on + strand, within Pf1N1B4_4506at 4968.930 kb on + strand, within Pf1N1B4_4506at 4968.931 kb on - strandat 4969.615 kb on - strand, within Pf1N1B4_4506at 4969.615 kb on - strand, within Pf1N1B4_4506at 4969.615 kb on - strand, within Pf1N1B4_4506at 4969.916 kb on + strandat 4970.488 kb on + strand, within Pf1N1B4_4507at 4970.489 kb on - strand, within Pf1N1B4_4507at 4970.489 kb on - strand, within Pf1N1B4_4507at 4970.489 kb on - strand, within Pf1N1B4_4507

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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4,967,732 - Pf1N1B4_4505 0.54 +0.5
4,968,066 - Pf1N1B4_4505 0.74 -1.5
4,968,695 - +0.5
4,968,753 - -0.7
4,968,810 + +0.1
4,968,930 + Pf1N1B4_4506 0.18 -1.6
4,968,930 + Pf1N1B4_4506 0.18 -1.8
4,968,931 - -3.6
4,969,615 - Pf1N1B4_4506 0.76 -0.5
4,969,615 - Pf1N1B4_4506 0.76 -3.9
4,969,615 - Pf1N1B4_4506 0.76 -0.7
4,969,916 + +1.5
4,970,488 + Pf1N1B4_4507 0.36 -0.3
4,970,489 - Pf1N1B4_4507 0.37 +0.8
4,970,489 - Pf1N1B4_4507 0.37 -1.8
4,970,489 - Pf1N1B4_4507 0.37 -2.7

Or see this region's nucleotide sequence