Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4172 and Pf1N1B4_4173 are separated by 55 nucleotides Pf1N1B4_4173 and Pf1N1B4_4174 are separated by 55 nucleotides Pf1N1B4_4174 and Pf1N1B4_4175 overlap by 15 nucleotides Pf1N1B4_4175 and Pf1N1B4_4176 overlap by 11 nucleotides
Pf1N1B4_4172 - hypothetical protein, at 4,622,770 to 4,622,904
_4172
Pf1N1B4_4173 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 4,622,960 to 4,623,523
_4173
Pf1N1B4_4174 - hypothetical protein, at 4,623,579 to 4,623,761
_4174
Pf1N1B4_4175 - Universal stress protein family 5, at 4,623,747 to 4,624,667
_4175
Pf1N1B4_4176 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,624,657 to 4,625,640
_4176
Position (kb) 4623
4624
4625 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.843 kb on + strand, within Pf1N1B4_4172 at 4622.967 kb on + strand at 4622.967 kb on + strand at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.261 kb on + strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.385 kb on - strand, within Pf1N1B4_4173 at 4623.667 kb on - strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.792 kb on + strand at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.869 kb on - strand, within Pf1N1B4_4175 at 4623.869 kb on - strand at 4624.306 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.330 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.543 kb on + strand at 4624.544 kb on - strand, within Pf1N1B4_4175 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.276 kb on + strand, within Pf1N1B4_4176 at 4625.555 kb on - strand at 4625.643 kb on + strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 4,622,837 + Pf1N1B4_4172 0.50 -0.3 4,622,837 + Pf1N1B4_4172 0.50 -1.3 4,622,838 - Pf1N1B4_4172 0.50 -0.6 4,622,838 - Pf1N1B4_4172 0.50 +0.2 4,622,843 + Pf1N1B4_4172 0.54 -0.4 4,622,967 + -0.9 4,622,967 + -2.6 4,623,075 + Pf1N1B4_4173 0.20 -0.4 4,623,075 + Pf1N1B4_4173 0.20 -0.9 4,623,076 - Pf1N1B4_4173 0.21 -0.7 4,623,076 - Pf1N1B4_4173 0.21 +0.6 4,623,261 + Pf1N1B4_4173 0.53 -1.4 4,623,262 - Pf1N1B4_4173 0.54 +0.1 4,623,262 - Pf1N1B4_4173 0.54 -0.9 4,623,262 - Pf1N1B4_4173 0.54 +0.3 4,623,262 - Pf1N1B4_4173 0.54 +2.2 4,623,262 - Pf1N1B4_4173 0.54 -2.0 4,623,262 - Pf1N1B4_4173 0.54 +2.1 4,623,262 - Pf1N1B4_4173 0.54 -0.5 4,623,385 - Pf1N1B4_4173 0.75 +0.1 4,623,667 - Pf1N1B4_4174 0.48 -1.1 4,623,709 + Pf1N1B4_4174 0.71 +0.6 4,623,709 + Pf1N1B4_4174 0.71 -1.8 4,623,792 + +0.8 4,623,868 + Pf1N1B4_4175 0.13 -0.4 4,623,868 + Pf1N1B4_4175 0.13 -1.7 4,623,868 + Pf1N1B4_4175 0.13 -0.8 4,623,868 + Pf1N1B4_4175 0.13 -2.4 4,623,869 - Pf1N1B4_4175 0.13 -0.6 4,623,869 - +0.4 4,624,306 + +0.6 4,624,329 + Pf1N1B4_4175 0.63 -2.4 4,624,329 + +0.6 4,624,329 + Pf1N1B4_4175 0.63 +0.6 4,624,329 + Pf1N1B4_4175 0.63 +0.6 4,624,330 - Pf1N1B4_4175 0.63 -1.5 4,624,525 - Pf1N1B4_4175 0.84 -0.4 4,624,525 - Pf1N1B4_4175 0.84 +0.6 4,624,543 + -2.6 4,624,544 - Pf1N1B4_4175 0.87 -2.3 4,624,764 + Pf1N1B4_4176 0.11 -0.7 4,624,764 + Pf1N1B4_4176 0.11 +0.6 4,625,036 + +0.6 4,625,036 + Pf1N1B4_4176 0.39 -0.6 4,625,036 + Pf1N1B4_4176 0.39 -3.7 4,625,076 - Pf1N1B4_4176 0.43 -0.1 4,625,076 - Pf1N1B4_4176 0.43 -1.4 4,625,276 + Pf1N1B4_4176 0.63 +0.6 4,625,555 - -0.6 4,625,643 + -2.4 4,625,644 - +1.2 4,625,644 - -3.3 4,625,644 - -1.0 4,625,644 - -1.6
Or see this region's nucleotide sequence