Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4126 and Pf1N1B4_4127 are separated by 3 nucleotides Pf1N1B4_4127 and Pf1N1B4_4128 are separated by 802 nucleotides
Pf1N1B4_4126 - Dihydroflavonol-4-reductase (EC 1.1.1.219), at 4,566,012 to 4,567,079
_4126
Pf1N1B4_4127 - hypothetical protein, at 4,567,083 to 4,567,502
_4127
Pf1N1B4_4128 - hypothetical protein, at 4,568,305 to 4,568,514
_4128
Position (kb)
4567
4568 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4566.195 kb on + strand at 4566.253 kb on + strand at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.253 kb on + strand, within Pf1N1B4_4126 at 4566.254 kb on - strand at 4566.254 kb on - strand at 4566.254 kb on - strand, within Pf1N1B4_4126 at 4566.281 kb on - strand, within Pf1N1B4_4126 at 4566.282 kb on + strand, within Pf1N1B4_4126 at 4566.297 kb on + strand, within Pf1N1B4_4126 at 4566.297 kb on + strand, within Pf1N1B4_4126 at 4566.297 kb on + strand, within Pf1N1B4_4126 at 4566.297 kb on + strand, within Pf1N1B4_4126 at 4566.327 kb on + strand, within Pf1N1B4_4126 at 4566.327 kb on + strand, within Pf1N1B4_4126 at 4566.328 kb on - strand, within Pf1N1B4_4126 at 4566.365 kb on + strand, within Pf1N1B4_4126 at 4566.365 kb on + strand, within Pf1N1B4_4126 at 4566.365 kb on + strand, within Pf1N1B4_4126 at 4566.366 kb on - strand, within Pf1N1B4_4126 at 4566.639 kb on - strand, within Pf1N1B4_4126 at 4566.646 kb on + strand at 4566.647 kb on - strand at 4566.647 kb on - strand, within Pf1N1B4_4126 at 4566.647 kb on - strand, within Pf1N1B4_4126 at 4566.732 kb on - strand at 4566.761 kb on - strand at 4566.761 kb on - strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.835 kb on + strand, within Pf1N1B4_4126 at 4566.836 kb on - strand, within Pf1N1B4_4126 at 4567.079 kb on + strand at 4567.079 kb on + strand at 4567.079 kb on + strand at 4567.079 kb on + strand at 4567.080 kb on - strand at 4567.080 kb on - strand at 4567.147 kb on - strand, within Pf1N1B4_4127 at 4567.183 kb on + strand, within Pf1N1B4_4127 at 4567.183 kb on + strand, within Pf1N1B4_4127 at 4567.184 kb on - strand, within Pf1N1B4_4127 at 4567.184 kb on - strand, within Pf1N1B4_4127 at 4567.184 kb on - strand, within Pf1N1B4_4127 at 4567.402 kb on + strand, within Pf1N1B4_4127 at 4567.403 kb on - strand at 4567.496 kb on + strand at 4567.497 kb on - strand at 4567.502 kb on + strand at 4567.502 kb on + strand at 4567.502 kb on + strand at 4567.502 kb on + strand at 4567.502 kb on + strand at 4567.502 kb on + strand at 4567.503 kb on - strand at 4567.503 kb on - strand at 4567.532 kb on + strand at 4567.533 kb on - strand at 4567.533 kb on - strand at 4567.536 kb on + strand at 4567.536 kb on + strand at 4567.536 kb on + strand at 4567.536 kb on + strand at 4567.536 kb on + strand at 4567.536 kb on + strand at 4567.537 kb on - strand at 4567.538 kb on + strand at 4567.538 kb on + strand at 4567.538 kb on + strand at 4567.538 kb on + strand at 4567.538 kb on + strand at 4567.539 kb on - strand at 4567.539 kb on - strand at 4567.577 kb on + strand at 4567.577 kb on + strand at 4567.610 kb on + strand at 4567.610 kb on + strand at 4567.610 kb on + strand at 4567.610 kb on + strand at 4567.720 kb on - strand at 4567.720 kb on - strand at 4567.898 kb on + strand at 4568.099 kb on + strand at 4568.206 kb on - strand at 4568.390 kb on - strand at 4568.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 4,566,195 + +0.6 4,566,253 + +1.6 4,566,253 + Pf1N1B4_4126 0.23 -2.1 4,566,253 + +0.6 4,566,253 + Pf1N1B4_4126 0.23 -1.0 4,566,253 + Pf1N1B4_4126 0.23 -0.4 4,566,253 + Pf1N1B4_4126 0.23 -0.4 4,566,253 + Pf1N1B4_4126 0.23 -1.7 4,566,253 + Pf1N1B4_4126 0.23 -1.0 4,566,253 + Pf1N1B4_4126 0.23 +0.6 4,566,254 - -0.4 4,566,254 - -2.4 4,566,254 - Pf1N1B4_4126 0.23 -0.8 4,566,281 - Pf1N1B4_4126 0.25 -1.0 4,566,282 + Pf1N1B4_4126 0.25 +0.6 4,566,297 + Pf1N1B4_4126 0.27 -2.0 4,566,297 + Pf1N1B4_4126 0.27 -2.7 4,566,297 + Pf1N1B4_4126 0.27 -2.6 4,566,297 + Pf1N1B4_4126 0.27 -0.4 4,566,327 + Pf1N1B4_4126 0.29 -1.0 4,566,327 + Pf1N1B4_4126 0.29 -1.1 4,566,328 - Pf1N1B4_4126 0.30 -0.8 4,566,365 + Pf1N1B4_4126 0.33 +0.2 4,566,365 + Pf1N1B4_4126 0.33 -0.0 4,566,365 + Pf1N1B4_4126 0.33 -0.5 4,566,366 - Pf1N1B4_4126 0.33 -1.0 4,566,639 - Pf1N1B4_4126 0.59 +0.3 4,566,646 + -0.1 4,566,647 - +0.6 4,566,647 - Pf1N1B4_4126 0.59 -3.2 4,566,647 - Pf1N1B4_4126 0.59 -2.6 4,566,732 - -1.7 4,566,761 - -1.7 4,566,761 - Pf1N1B4_4126 0.70 -0.2 4,566,835 + Pf1N1B4_4126 0.77 -2.7 4,566,835 + Pf1N1B4_4126 0.77 -0.3 4,566,835 + Pf1N1B4_4126 0.77 -1.4 4,566,835 + Pf1N1B4_4126 0.77 +0.6 4,566,835 + Pf1N1B4_4126 0.77 +0.6 4,566,835 + Pf1N1B4_4126 0.77 -1.0 4,566,836 - Pf1N1B4_4126 0.77 -1.3 4,567,079 + +0.8 4,567,079 + +0.6 4,567,079 + -1.3 4,567,079 + -0.9 4,567,080 - -0.4 4,567,080 - -1.0 4,567,147 - Pf1N1B4_4127 0.15 -1.1 4,567,183 + Pf1N1B4_4127 0.24 +1.2 4,567,183 + Pf1N1B4_4127 0.24 -2.7 4,567,184 - Pf1N1B4_4127 0.24 -0.7 4,567,184 - Pf1N1B4_4127 0.24 -2.7 4,567,184 - Pf1N1B4_4127 0.24 -2.0 4,567,402 + Pf1N1B4_4127 0.76 +0.6 4,567,403 - -2.0 4,567,496 + +0.6 4,567,497 - -0.4 4,567,502 + -0.2 4,567,502 + -0.0 4,567,502 + -3.0 4,567,502 + +0.1 4,567,502 + -2.4 4,567,502 + -0.1 4,567,503 - -1.7 4,567,503 - -3.4 4,567,532 + -1.4 4,567,533 - -1.7 4,567,533 - -0.4 4,567,536 + +0.6 4,567,536 + -2.7 4,567,536 + -1.4 4,567,536 + +0.6 4,567,536 + -0.4 4,567,536 + -1.4 4,567,537 - -2.4 4,567,538 + -2.4 4,567,538 + -3.1 4,567,538 + -0.3 4,567,538 + +0.1 4,567,538 + +1.0 4,567,539 - -2.0 4,567,539 - -2.0 4,567,577 + +0.6 4,567,577 + -1.0 4,567,610 + +0.6 4,567,610 + +0.7 4,567,610 + +0.6 4,567,610 + +0.4 4,567,720 - +0.6 4,567,720 - -0.7 4,567,898 + +0.6 4,568,099 + -0.4 4,568,206 - +0.6 4,568,390 - +0.6 4,568,502 + +0.6
Or see this region's nucleotide sequence