Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_400 and Pf1N1B4_401 are separated by 215 nucleotides Pf1N1B4_401 and Pf1N1B4_402 are separated by 220 nucleotides Pf1N1B4_402 and Pf1N1B4_403 are separated by 462 nucleotides Pf1N1B4_403 and Pf1N1B4_404 are separated by 112 nucleotides
Pf1N1B4_400 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 469,384 to 470,376
_400
Pf1N1B4_401 - GCN5-related N-acetyltransferase (EC 2.3.1.57), at 470,592 to 471,074
_401
Pf1N1B4_402 - metal-dependent phosphohydrolase, at 471,295 to 471,951
_402
Pf1N1B4_403 - FIG00965925: hypothetical protein, at 472,414 to 472,668
_403
Pf1N1B4_404 - ParE toxin protein, at 472,781 to 473,161
_404
Position (kb) 471
472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.325 kb on - strand at 470.332 kb on + strand at 470.332 kb on + strand at 470.333 kb on - strand at 470.418 kb on + strand at 470.419 kb on - strand at 470.420 kb on + strand at 470.421 kb on - strand at 470.436 kb on + strand at 470.447 kb on - strand at 470.448 kb on + strand at 470.450 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.558 kb on + strand at 470.559 kb on - strand at 470.559 kb on - strand at 470.680 kb on + strand, within Pf1N1B4_401 at 470.681 kb on - strand, within Pf1N1B4_401 at 470.681 kb on - strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.831 kb on - strand, within Pf1N1B4_401 at 471.107 kb on - strand at 471.107 kb on - strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.161 kb on + strand at 471.161 kb on + strand at 471.181 kb on + strand at 471.181 kb on + strand at 471.181 kb on + strand at 471.182 kb on - strand at 471.217 kb on - strand at 471.363 kb on + strand, within Pf1N1B4_402 at 471.364 kb on - strand, within Pf1N1B4_402 at 471.564 kb on - strand, within Pf1N1B4_402 at 471.591 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.755 kb on - strand at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.790 kb on + strand, within Pf1N1B4_402 at 471.949 kb on + strand at 471.949 kb on + strand at 472.025 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.261 kb on - strand at 472.261 kb on - strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.386 kb on - strand at 472.386 kb on - strand at 472.410 kb on - strand at 472.425 kb on + strand at 472.460 kb on + strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.667 kb on - strand at 472.674 kb on + strand at 472.762 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.851 kb on - strand, within Pf1N1B4_404
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 470,324 + -1.9 470,324 + +1.5 470,324 + -0.3 470,324 + -0.6 470,325 - -0.3 470,332 + -3.6 470,332 + -1.0 470,333 - +0.7 470,418 + -0.6 470,419 - +1.2 470,420 + +0.5 470,421 - -1.7 470,436 + -1.3 470,447 - -2.1 470,448 + -0.9 470,450 + -2.1 470,523 + -1.0 470,523 + -0.5 470,523 + -0.6 470,523 + -1.3 470,523 + -2.8 470,558 + -0.6 470,559 - +0.2 470,559 - +0.2 470,680 + Pf1N1B4_401 0.18 +0.7 470,681 - Pf1N1B4_401 0.18 +2.0 470,681 - Pf1N1B4_401 0.18 -1.4 470,815 + Pf1N1B4_401 0.46 +0.2 470,815 + Pf1N1B4_401 0.46 -0.7 470,815 + Pf1N1B4_401 0.46 -3.2 470,815 + Pf1N1B4_401 0.46 +0.7 470,815 + Pf1N1B4_401 0.46 -0.1 470,816 - Pf1N1B4_401 0.46 -1.0 470,816 - Pf1N1B4_401 0.46 -0.8 470,816 - Pf1N1B4_401 0.46 -3.1 470,816 - Pf1N1B4_401 0.46 -0.4 470,816 - Pf1N1B4_401 0.46 -0.9 470,816 - Pf1N1B4_401 0.46 +0.7 470,831 - Pf1N1B4_401 0.49 +0.7 471,107 - +0.6 471,107 - -0.9 471,109 + -0.5 471,109 + -1.6 471,109 + -1.7 471,109 + -1.3 471,109 + +0.7 471,109 + +0.0 471,109 + -3.4 471,109 + -1.6 471,109 + -1.0 471,109 + +0.7 471,110 - -0.3 471,110 - -2.1 471,110 - -2.1 471,110 - -1.3 471,155 + +0.3 471,155 + -2.6 471,155 + +0.7 471,155 + +0.7 471,155 + -2.0 471,155 + -1.1 471,156 - -2.6 471,156 - -1.5 471,156 - +0.9 471,156 - -1.6 471,161 + +0.7 471,161 + -1.3 471,181 + -3.0 471,181 + +0.7 471,181 + +0.4 471,182 - -0.3 471,217 - -0.1 471,363 + Pf1N1B4_402 0.10 +0.7 471,364 - Pf1N1B4_402 0.11 +0.7 471,564 - Pf1N1B4_402 0.41 -0.7 471,591 + Pf1N1B4_402 0.45 +1.5 471,724 + +0.7 471,724 + Pf1N1B4_402 0.65 -0.5 471,724 + Pf1N1B4_402 0.65 -1.5 471,724 + Pf1N1B4_402 0.65 -3.6 471,724 + Pf1N1B4_402 0.65 +0.7 471,725 - Pf1N1B4_402 0.65 -2.4 471,725 - Pf1N1B4_402 0.65 -2.2 471,725 - Pf1N1B4_402 0.65 -1.2 471,725 - Pf1N1B4_402 0.65 -0.4 471,725 - Pf1N1B4_402 0.65 -2.3 471,754 + Pf1N1B4_402 0.70 -3.1 471,754 + Pf1N1B4_402 0.70 +0.7 471,754 + Pf1N1B4_402 0.70 -1.6 471,755 - -2.7 471,755 - Pf1N1B4_402 0.70 +0.7 471,755 - Pf1N1B4_402 0.70 -0.3 471,755 - Pf1N1B4_402 0.70 +0.7 471,790 + Pf1N1B4_402 0.75 -0.1 471,949 + -2.7 471,949 + -0.6 472,025 + -0.9 472,260 + -0.4 472,260 + -0.6 472,260 + -0.9 472,260 + +0.1 472,261 - -0.3 472,261 - -0.9 472,385 + -2.9 472,385 + -2.1 472,385 + -1.4 472,385 + -3.0 472,386 - -2.7 472,386 - -1.9 472,410 - +0.0 472,425 + -3.4 472,460 + Pf1N1B4_403 0.18 +1.1 472,461 - Pf1N1B4_403 0.18 -0.5 472,461 - Pf1N1B4_403 0.18 -0.5 472,461 - Pf1N1B4_403 0.18 -1.3 472,667 - -0.7 472,674 + +0.7 472,762 + +1.2 472,805 + +0.7 472,805 + -1.9 472,805 + -0.4 472,806 - +0.7 472,806 - +0.2 472,806 - +2.0 472,850 + Pf1N1B4_404 0.18 +0.7 472,850 + Pf1N1B4_404 0.18 -1.3 472,850 + Pf1N1B4_404 0.18 -0.9 472,851 - Pf1N1B4_404 0.18 +0.7
Or see this region's nucleotide sequence