Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3966 and Pf1N1B4_3967 are separated by 215 nucleotides Pf1N1B4_3967 and Pf1N1B4_3968 are separated by 228 nucleotides
Pf1N1B4_3966 - serine/threonine protein kinase, at 4,406,504 to 4,407,805
_3966
Pf1N1B4_3967 - AFG1-like ATPase, at 4,408,021 to 4,409,151
_3967
Pf1N1B4_3968 - Spermidine/putrescine-binding periplasmic protein, at 4,409,380 to 4,410,423
_3968
Position (kb)
4408
4409
4410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4407.102 kb on + strand, within Pf1N1B4_3966 at 4407.267 kb on + strand, within Pf1N1B4_3966 at 4407.268 kb on - strand, within Pf1N1B4_3966 at 4407.268 kb on - strand, within Pf1N1B4_3966 at 4408.151 kb on + strand, within Pf1N1B4_3967 at 4408.151 kb on + strand, within Pf1N1B4_3967 at 4408.151 kb on + strand, within Pf1N1B4_3967 at 4408.151 kb on + strand, within Pf1N1B4_3967 at 4408.152 kb on - strand at 4408.152 kb on - strand at 4408.262 kb on + strand, within Pf1N1B4_3967 at 4408.263 kb on - strand, within Pf1N1B4_3967 at 4408.436 kb on + strand, within Pf1N1B4_3967 at 4408.895 kb on + strand at 4408.895 kb on + strand, within Pf1N1B4_3967 at 4408.895 kb on + strand, within Pf1N1B4_3967 at 4408.896 kb on - strand, within Pf1N1B4_3967 at 4408.896 kb on - strand, within Pf1N1B4_3967 at 4408.997 kb on + strand, within Pf1N1B4_3967 at 4409.027 kb on + strand, within Pf1N1B4_3967 at 4409.027 kb on + strand, within Pf1N1B4_3967 at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.119 kb on - strand at 4409.173 kb on + strand at 4409.201 kb on - strand at 4409.201 kb on - strand at 4409.201 kb on - strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.248 kb on - strand at 4409.515 kb on - strand, within Pf1N1B4_3968 at 4409.685 kb on - strand, within Pf1N1B4_3968 at 4409.798 kb on + strand, within Pf1N1B4_3968 at 4409.799 kb on - strand, within Pf1N1B4_3968 at 4410.023 kb on + strand, within Pf1N1B4_3968 at 4410.023 kb on + strand, within Pf1N1B4_3968 at 4410.024 kb on - strand, within Pf1N1B4_3968 at 4410.150 kb on - strand, within Pf1N1B4_3968
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 4,407,102 + Pf1N1B4_3966 0.46 +0.4 4,407,267 + Pf1N1B4_3966 0.59 +0.7 4,407,268 - Pf1N1B4_3966 0.59 +0.7 4,407,268 - Pf1N1B4_3966 0.59 +1.0 4,408,151 + Pf1N1B4_3967 0.11 -1.7 4,408,151 + Pf1N1B4_3967 0.11 +0.7 4,408,151 + Pf1N1B4_3967 0.11 -0.2 4,408,151 + Pf1N1B4_3967 0.11 -0.6 4,408,152 - +0.7 4,408,152 - -0.6 4,408,262 + Pf1N1B4_3967 0.21 -0.3 4,408,263 - Pf1N1B4_3967 0.21 +0.7 4,408,436 + Pf1N1B4_3967 0.37 +0.8 4,408,895 + +0.2 4,408,895 + Pf1N1B4_3967 0.77 +0.7 4,408,895 + Pf1N1B4_3967 0.77 +0.7 4,408,896 - Pf1N1B4_3967 0.77 -2.9 4,408,896 - Pf1N1B4_3967 0.77 +1.0 4,408,997 + Pf1N1B4_3967 0.86 +2.2 4,409,027 + Pf1N1B4_3967 0.89 +1.5 4,409,027 + Pf1N1B4_3967 0.89 -1.8 4,409,118 + -0.9 4,409,118 + +0.4 4,409,118 + -0.7 4,409,118 + -0.2 4,409,118 + +1.7 4,409,118 + +2.2 4,409,119 - -2.1 4,409,173 + -0.8 4,409,201 - -1.0 4,409,201 - -0.5 4,409,201 - +0.7 4,409,247 + -3.5 4,409,247 + -0.2 4,409,247 + -3.0 4,409,247 + -0.8 4,409,247 + -1.1 4,409,247 + +0.7 4,409,248 - -2.8 4,409,515 - Pf1N1B4_3968 0.13 +0.7 4,409,685 - Pf1N1B4_3968 0.29 -1.5 4,409,798 + Pf1N1B4_3968 0.40 -0.2 4,409,799 - Pf1N1B4_3968 0.40 +0.5 4,410,023 + Pf1N1B4_3968 0.62 -0.3 4,410,023 + Pf1N1B4_3968 0.62 +0.7 4,410,024 - Pf1N1B4_3968 0.62 +0.2 4,410,150 - Pf1N1B4_3968 0.74 +0.7
Or see this region's nucleotide sequence